Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,897,819	G→A	V296V (GTG→GTA)	sdaA →	L‑serine deaminase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,897,819	0	G	A	100.0%	10.0 / NA	5	V296V (GTG→GTA)	sdaA	L‑serine deaminase I
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (2/3);  total (2/3)

GCCGGATGCTGGTTTCCAGCGATAAACTGTCTAACGATCCGATGAATGTCATTGACTGGGTAAACATGTTTGCGCTGGCAGTTAACGAAGAAAACGCCGCCGGTGGTCGTGTGGTAACTGCGCCAACCAACGGTGCCTGCGGTATCGTTCCGGCAGTGCTGGCTTACTATGACCACTTTATTGAATCGGTCAGCCCGGACATCTATACCCGTTACTTTATGGCAGCGGGCGCGATTGGTGCATTGTATAAAATGAACGCCTCTATTTCCGGTGCGGAAGTTGGTTGCCAGGGCGAAGTGG  >  NC_000913/1897662‑1897961                                                                                                                                                              |                                                                                                                                               gcCGGATGCTGGTTTCCAGCGATAAACTGTCTAACGATCCGATGAATGTCATTGACTGGGTAAACATGTTTGCGCTGGCAGTTAACGAAGAAAACGCCGCCGGTGGTCGTGTGGTAACTGCGCCAACCAACGGTGCCTGCGGTATCGTTCCGGCAGTACTGGCTTACTATGACCACTTTATTGAATCGGTCAGCCCGGACATCTATACCCGTTACttt                                                                                    >  1:50660/1‑218 (MQ=255)                          nctntcgnanntnncGANGAAGGGCATGGACGGGGTAAANNTGTTTGCGCNGGCAGTTAACGAAGAAAACGCCGCCGGTGGTCGTGTGGTAACTGCGCCAACCANCGGTGCTTGNNGTNTTGTGNCNNNANNNCTNGCTGACTATGACCACTTTANTGNNTCGGTCNGCCCGGACATCTATACCCGTTACttt                                                                                    <  2:50660/179‑1 (MQ=255)                                            gAATGTCATTGACTGGGTAAACATGTTTGCGCTGGCAGTTAACGAAGAAAACGCCGCCGGTGGTCGTGTGGTAACTGCGCCAACCAACGGTGCCTGCGGTATCGTTCCGGCAGTACTGGCTTACTATGACCACTTTATTGAATCGGTCAGCCCGGACATCTATACCCGTTACTTTATGGCAGCGGGCGCGATTGGTGCATTGTATAAAATGAACGCCTCTATTTCCGGTGCGGAAGTTGGTTGCCAGGNc         <  1:170828/250‑1 (MQ=255)                                                                                 gTTAACGAAGAAAACGCCGCCGGTGGTCGTGTGGTAACTGCGCCAACCAACGGTGCCTGCGGTAGCGTTCCGGCAGTACTGGCTTAc                                                                                                                                       <  1:75423/87‑1 (MQ=255)                                                                                                                         cgcCAACCAACGGTGCCTGCGGTATCGATCCGGCAGTACTGGCTTACTATGACCACTTTCTTGAATCGGTCTGCCCGGACATCtat                                                                                                >  1:123483/1‑86 (MQ=255)                                                                                                                                     gTGCCTGCGGTATCGTTCCGGCAGTACTGGCTTACTATGACCACTTTATTGAATCGGTCAGCCCGGACATCTATACCCGTTACTTTATGGCAGCGGGCGCGATTGGTGCATTGTATAAAATGAACGCCTCTATTTCCGGTGCGGAAGTTGGTTGCCAGGGCGAAGTgn  <  1:38329/168‑2 (MQ=255)                                                                                                                                                              |                                                                                                                                               GCCGGATGCTGGTTTCCAGCGATAAACTGTCTAACGATCCGATGAATGTCATTGACTGGGTAAACATGTTTGCGCTGGCAGTTAACGAAGAAAACGCCGCCGGTGGTCGTGTGGTAACTGCGCCAACCAACGGTGCCTGCGGTATCGTTCCGGCAGTGCTGGCTTACTATGACCACTTTATTGAATCGGTCAGCCCGGACATCTATACCCGTTACTTTATGGCAGCGGGCGCGATTGGTGCATTGTATAAAATGAACGCCTCTATTTCCGGTGCGGAAGTTGGTTGCCAGGGCGAAGTGG  >  NC_000913/1897662‑1897961

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑