Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,893,700	G→A	A112T (GCT→ACT)	yoaB →	RutC family protein YoaB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,893,700	0	G	A	88.9%	13.4 / ‑5.0	9	A112T (GCT→ACT)	yoaB	RutC family protein YoaB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (4/4);  minor base C (1/0);  total (5/4)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.79e-01

ACACGCTGGCACAGATTGACGCCGTGCTGGAAAAACAGGGCAGCAATAAATCGAGCATTCTGGATGCCACCATTTTCCTGGCCGATAAAAACGACTTCGCGGCGATGAATAAAGCGTGGGATGCTTGGGTTGTCGCGGGTCATGCGCCGGTGCGCTGCACGGTACAAGCGGGTTTGATGAACCCGAAGTATAAAGTTGAAATTAAGATTGTGGCTGCGGTGTAAGCTTTATCGAAGCAAAATAAGTCAGACGATAATTTATCGATAATACTGGTCGGTTTTACATAAATCGACCAGAGAATAAGATATTACTGTTGATGCTTCTGCTAATTCATTATTTATATTTATAATTTCAATTTTATCTATAAAACTAACTATATAAATAAACATAAACATGCCGTTGTGTTCGCCCTTTGATTAGCAACTCTGGTTTTCTTTCTTAG  >  NC_000913/1893488‑1893929                                                                                                                                                                                                                     |                                                                                                                                                                                                                                      acacGCTGGCACAGATTGACGCCGTGCTGGTAAAACAGGGCAGCAATAAATCGAGCATTCTGGATGCCTCCATTTTCCTGGCCGAGAAAAACGACTTCGCGGCGATGAATAAAGCGTGGGATGCTTGGGTTGTCGCGGGTCATGCGCCGGTGCGCTGATCGGTACAAGCGGGTTTGATTAAGCCGAAGTATAAAGTGGAAATTAAGATTGTGACTGCGGTGTAAGCTTTATCGAAGCAAAATAAGTCAGAc                                                                                                                                                                                                 <  1:109470/251‑1 (MQ=255) acacGCTGGCACAGATTGACGCCGTGCTGGAAAATCAGGGCAGCAATAAATCGAGCATTCTGGATGCCACCATTTTCCTGGCCGATAAAAACGACTTCGCGGCGATGAATAAAGCGTGGGATGCTTGGGTTGTCGCGGTTCATGCGCCGGTGCGCTGCACGGTACAAGCGGGTTGGATGAACCCGAAGTATAAAGTTGAAAGTAAGATTGTGACTGCGGTGTAAGCTTTATCGAAGCAAAATAAGTCAGAc                                                                                                                                                                                                 <  1:119560/251‑1 (MQ=255)                                                                                                           gAATAAAGCGTGGGATGCTTGGGTTGTCGCGGGTCATGCGCCGGTGCGCGGCACGGTACAAGCGGGTTTGATGAACCCGAAGTATAAAGTTGAAATTAAGATTGTGACTGCGGTGTAAGCTTTATCGAAGCCAAATAAGTCa                                                                                                                                                                                                    >  1:27497/1‑142 (MQ=255)                                                                                                             aTAAAGCGTGGGATGCTTGGGTTGTCGCGGGTCATGCGCCGGTGCGCTGCACGGTACAAGCGGGTTTGATGAACCCGAAGTATAAAGTTGAAATTAAGATTGTGACTGCGGTGTAAGCTTTATCGAAGCAAAATAAGTCAGACGATAATTTATCGATAATACTGGTCGGTTTTACATAAATCGACCAGAGAATAAGATATTACTGTTGATGCTTCTGCTAATTCATTATTTATATTTATAATTTCAAtttt                                                                                     >  1:13081/1‑251 (MQ=255)                                                                                                                                            tCATGCGCCGGTGCGCTGCACGGGACAAGCGGGTTTGATGAACCCGAAGTATAAAGTTGAAATTAAGCCTGTGCCGGGGGTGTAAGCTTTATCGAAGCAAAATAAGTCAGACGATAATTTATCGATAATACTGGTCGGTTTTACATAATACGACCAGAGACTAAGATATTACTGTTGATGCTTCTGCTAATTCATTATTTATATTTATAATTTCAATTTTATCTATAAAACTAACTATATAAataaacat                                                       >  1:215792/1‑250 (MQ=255)                                                                                                                                                                         cGGGTTTGATGAACCCGAAGTCTAAAGTTGAAATTAAGATTGTGACTGCGGTGTAAGCTTTATCGAAGCAAAATAAGTCAGACGATAATTTATCGATAATACTGGTCGGTTTTACATAAATCGACCAGAGAATAAGATATTACTGTTGATGCTTCTGCTAATTCATTATTTATATTTATAATTTCAATTTTATCTATAAaactaac                                                                      >  1:67189/1‑206 (MQ=255)                                                                                                                                                                                    aacacGAAGTATAAAGTTGAAAGTAAGATGGTGACGGCGGTGTAAGCTTTATCGAAGCAAAATAAGTCAGACGATAATTTATCGATAATATTGGTCGTTTTTACATAAATCGACCAGAGAATAAGATATTACTGGTGATGCTTCTGCTAATGCATTATTTATATTTATAATTTCAATTTTACCTATAAAACTAACGAAATAACTAAACAGAACCATGCCGTTGTGTTCGCCCTGTGATTAGTAACTCTGGt              <  1:145339/247‑1 (MQ=255)                                                                                                                                                                                       ccGAAGTATAAAGTTGAAATTAAGATTGTGACTGCGGTGTAAGCTTTATCGAAGCAAAATAAGTCAGACGATACTTTATCGATAATACTGGTCGGTTTTACATAAATCGACCAGAGAATAAGATATTACTGTTGATGCTTCTGCTAATCCATCATTTATATTTATAATTTCAATTTTATCTATAAAACTAACTATATAAATAAACATAAACATGCCGTTGTGTTCGCCCTTTGATTAGCAACTCTGGtttt           >  1:217817/1‑251 (MQ=255)                                                                                                                                                                                                aaaGTTGAAATTAAGATTGTGACTGCGGTGTAAGCTTTATCGAAGCAAAATAAGTCAGACGATAATTTATCGATAATACTGGTCGGTTTTACATAAATCGACCAGAGAATAAGATATTACTGTTGATGCTTCTGCTAATTCATTATTTATATTTATAATTTCAATTTTATCTATAAAACTAACTATATAAATAAACATAAACATGCCGTTGTGTTCGCCCTTTGATTAGCAACTCTGGTTTTCTTTCTTAg  <  1:61693/251‑1 (MQ=255)                                                                                                                                                                                                                     |                                                                                                                                                                                                                                      ACACGCTGGCACAGATTGACGCCGTGCTGGAAAAACAGGGCAGCAATAAATCGAGCATTCTGGATGCCACCATTTTCCTGGCCGATAAAAACGACTTCGCGGCGATGAATAAAGCGTGGGATGCTTGGGTTGTCGCGGGTCATGCGCCGGTGCGCTGCACGGTACAAGCGGGTTTGATGAACCCGAAGTATAAAGTTGAAATTAAGATTGTGGCTGCGGTGTAAGCTTTATCGAAGCAAAATAAGTCAGACGATAATTTATCGATAATACTGGTCGGTTTTACATAAATCGACCAGAGAATAAGATATTACTGTTGATGCTTCTGCTAATTCATTATTTATATTTATAATTTCAATTTTATCTATAAAACTAACTATATAAATAAACATAAACATGCCGTTGTGTTCGCCCTTTGATTAGCAACTCTGGTTTTCTTTCTTAG  >  NC_000913/1893488‑1893929

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑