Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,882,236 G→A V109I (GTT→ATT)  dmlA → D‑malate/3‑isopropylmalate dehydrogenase (decarboxylating)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,882,2360GA100.0% 18.7 / NA 9V109I (GTT→ATT) dmlAD‑malate/3‑isopropylmalate dehydrogenase (decarboxylating)
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (4/5);  total (4/5)

CGCTGAGCGCTGGGGCTTCGCCTTGAGTTTTGAGCAAATGGAGTGGGCGAGCTGCGAGTATTACAGCCATCACGGTAAAATGATGCCGGACGACTGGCATGAGCAACTTAGCCGTTTCGACGCCATCTATTTTGGTGCCGTCGGCTGGCCGGATACCGTTCCGGACCATATTTCGTTGTGGGGTTCGCTGCTGAAATTTCGTCGTGAATTCGACCAGTACGTCAACCTGCGCCCGGTTCGTCTCTTTCCTGGCGTTCCCTGCCCGCTGGCGGGAAAACAGCCTGGCGACATCGATTTTTACGTGGTCAGGGAAAACACCGAAGGCGAATATTCCTCGCTCGGCGGTAGAGTGAATGAAGGTACAGAGCATGAAGTCGTCATTCAGGAATCGGTATTTACCCGCCGTGGTGTCGATCGCATTTTGCGTTATGCCTTCGAACTTGCGCAAAGCCGT  >  NC_000913/1882001‑1882454
                                                                                                                                                                                                                                           |                                                                                                                                                                                                                          
cGCTGAGCGCTGGGGCTTCGCCTTGAGTTTTGAGCAAATGGAGTGGGCGAGCTGCGAATATTACAGCCATCACGGTAAAATGATGCCGGACGACTGGCATGAGCAACTTAGCCGTTGCGACGCCATCTATTTTGGTGCCGTCGGCTGGCCGGATACCGTTCCGTACCATATTGCGTTGTGGGGTTCGCTGCTGAAATTTCGTCGTGAATTCGACCAGTACGTCATCCTGCGCCCGATTCGTCTCTTTCCTg                                                                                                                                                                                                             <  1:186371/251‑1 (MQ=255)
                                                               naGCCATCACGGTAAAATGATGCCGGACGACTGGCATGAGCAACTTAGCCGTTTCGACGCCATCTATTTTGGTGCCGTCGGCTGGCCGGATACCGTTCCGGACCATATTTCGTTGTGGGGTTCGCTGCTGAAATTTCGTCGTGAATTCGACCAGTACGTCAACCTGCGCCCGATTCGTCTCTTTCCTGGCGTTCCCTGCCCGCTGGCGGGAAAACAGCCTGGCGACATCGATTTTTACGTGGTCAGGGaa                                                                                                                                               >  1:57144/2‑250 (MQ=255)
                                                                        cGGTAAAATGATGCCGGACGACTGGCATGAGCAACTTAGCCGTTTCGACGCCATCTATTTTGGTGCCGTCGGCTGGCCGGATACCGTTCCGGACCATATTTCGTTGTGGGGTTCGCTGCTGAAATTTCGTCGTGAATTCGACCAGTACGTCAACCTGCGCCCGATTCGTCTCTTTCCTGGCg                                                                                                                                                                                                          >  1:74325/1‑182 (MQ=255)
                                                                                                                                      gTGCCGTCGGCTGGCCGGATACCGTTCCGGACCATATTTCGTTGTGGGGTTCGCTGCTGAAATTTCGTCGTGAATTCGACCAGTACGTCAACCTGCGCCCGATTCGTCTCTTTCCTGGCGTTCCCTGCCCGCTGGCGGGAAAACAGCCTGGCGACATCGATTTTTACGTGGTCAGGGAAAACACCGAAGGCGAATATTCCTCGCTCGGCGGTAGAGTGAATGAAGGTACAGAGCATGAAGTCGTCATTCAg                                                                       <  1:59671/251‑1 (MQ=255)
                                                                                                                                                                               ttgtggggtgcgttcgtgAAATTTCTTCGTGAATCCGACCAGTCCGCCACCCTGCGCCCGATTCGTCTCTTTCCTGGCGTTCCCTGCCCGCTGGCGGGAAAACAGCCGGGCGACATCTATTTTTCCGTGGTCAGGAAAAACCCCAAAGGCAAATATCCCTCGCTCGGGGGTAGAGTGAATGAAGGTCCAGAGCATGAAGTCTTCATTCAGGAATCGGTATTTACCCGCCGTGGTGTCGATCGCAtttn                                 <  1:130091/232‑2 (MQ=255)
                                                                                                                                                                                           ctgctgAAATTTCGTCGTGAATTCGACCAGTACGTCAACCTGCGCCCGATTCGTCTCTTTCCTGGCGTTCCCTGCCCGCTGGCGGGAAAACAGACTGGCGACATCGATTTTTCCGTGGTCCGGGAAAACACCGAAGGCGAATATTCCTTGCTCGGCGGTAGAGTGAATGAAGGTACAGAGCATGa                                                                                    >  1:217088/1‑185 (MQ=255)
                                                                                                                                                                                             gctgAAATTTCGTCGTGAATTCGACCAGTACGTCAACCTGCGCCCGATTCGTCTCTTTCCTGGCGTTCCCTGCCCGCTGGCGGGAAAACAGCCTGGCGACATCGATTTTTACGTGGTCAGGGAAAACACCGAAGGCGAATATTCCTCGCTCGGCGGTAGAGTGAATGAAGGTACAGAGCATGAAGTCGTCATTCAg                                                                       <  1:32830/196‑1 (MQ=255)
                                                                                                                                                                                                           gtGAATTCGACCAGTACGTCAACCTGCGCCCGATTCGTCTCTTTCCTGGCGTTCCCTGCCCGCTGGCGGGAAAACAGc                                                                                                                                                                               <  1:16726/78‑1 (MQ=255)
                                                                                                                                                                                                           gggAATTCGACCAGTACGTCAACCTGCGCCCGATTCGAATCTTTCCTGGCGTTCCCTGCCCGCTGGCGGTAACACAGCCTGGCGACATCGATTTTTCCGTGGCCAGGGAAAACACCGCAGGCGAATCTTCCTCGCTCGGAGGTAGAGTGAATGAAGGGACAGAGCATGAAGTCGTCATTCAGGAATCGGTATTTACCCGCCGTGGGGTCGATGGCAGTTTGAGCTTTGCCTTCGAAATTGCGGCAAGCCgg  >  1:96718/3‑250 (MQ=255)
                                                                                                                                                                                                                                           |                                                                                                                                                                                                                          
CGCTGAGCGCTGGGGCTTCGCCTTGAGTTTTGAGCAAATGGAGTGGGCGAGCTGCGAGTATTACAGCCATCACGGTAAAATGATGCCGGACGACTGGCATGAGCAACTTAGCCGTTTCGACGCCATCTATTTTGGTGCCGTCGGCTGGCCGGATACCGTTCCGGACCATATTTCGTTGTGGGGTTCGCTGCTGAAATTTCGTCGTGAATTCGACCAGTACGTCAACCTGCGCCCGGTTCGTCTCTTTCCTGGCGTTCCCTGCCCGCTGGCGGGAAAACAGCCTGGCGACATCGATTTTTACGTGGTCAGGGAAAACACCGAAGGCGAATATTCCTCGCTCGGCGGTAGAGTGAATGAAGGTACAGAGCATGAAGTCGTCATTCAGGAATCGGTATTTACCCGCCGTGGTGTCGATCGCATTTTGCGTTATGCCTTCGAACTTGCGCAAAGCCGT  >  NC_000913/1882001‑1882454

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑