Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,874,106	G→A	S26F (TCC→TTC)	yoaI ←	protein YoaI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,874,106	0	G	A	100.0%	18.7 / NA	9	S26F (TCC→TTC)	yoaI	protein YoaI
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (4/5);  total (4/5)

GTCTTCGGCGCGATCCCCCCTTTCAGCTTCCATCCAAAACTCAAACTGGTTGCCGACCCTCTACTCTTCGAACGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAACTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACA‑GCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCCCCGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAGTTCGCCTGATTTTTTATGCAAAAAACGTAAAGATTTTCTACTTCCTTCCTGCAGCAAGCGTAAAGTAAGCAGGCTTATTATTTT  >  NC_000913/1873889‑1874334                                                                                                                                                                                                                          |                                                                                                                                                                                                                                      gtCTTCGTCGCGATCCCCCCTTTCAGCTTCCATCCAAAACTCAAACTGGTTGCCGACCCTCTACTCTTCGAACGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGAACTGGTCAAGGTCCGCCGCACTGCGTAACTAGCCTGTCCGTTCGATAAGAAGAACGAACAAAACCAGTACAACA‑Gc                                                                                                                                                                                                                   <  1:195897/237‑1 (MQ=255)           cgATACCCCCTTTCAGCTTCAATACAAAACTCAAACTGGTGGCCGACCCTCTACTCTTCGAACGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGAACTGGTTAATTTCCGCCGAACTGAGTAACTAGCCGGTCCGTCCGATAAGAAGAATGAACAAAAACAGTACAACA‑GCAATGGAAAAAAACGATGACGTGa                                                                                                                                                                                            >  1:27453/1‑250 (MQ=255)                cccccTTCCAGCTCCCATCAAAAACTCAAACGGGTTGCCGACCCGCTGCTTTTCGAACGATTTGATGAAAGCGCTTTCAAGGCGGGCATGCTGGAAAAATCCGGTATGTTGAAAACCGGGGATTATTTGCTGATTGCGCAACCAGAATGGGTCATTGCCCGCCGCACTGCGTAAGTAGCCGGTCCGTTCGATAAGAAGAACGAACAAAACCa                                                                                                                                                                                                                              <  1:120516/212‑1 (MQ=255)                                                                                       cGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAACTAGCCGGTCCGTTCGATAAGAAGAACGAACAAAACCAGTACAACA‑GCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATTCCCCCGGGAGATAGCTGTTTTCACGTTATTGTTAGCg                                                                                                               <  1:164790/251‑1 (MQ=255)                                                                                             cAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAACTAGCCGGTCCGTTCGATAAGAAGAACGAACAAAACCAGTACAACA‑GCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCCCCGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGcac                                                                                                          <  1:185497/250‑1 (MQ=255)                                                                                                                                                 cgcattgcgcaACCAGAACTGGTCACTTTCCGCCGCACTGCGTAACTAGCCGGTCCGTTCGATACGAAGAACGAACAAAACAAGT‑CAAAACGCCATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACACACATTTGATCGTTCCTAGCATGCCCCCGAGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAGTTCGCCTGATTTTTTATGCAAAAAACGTaa                                                        >  1:112602/1‑248 (MQ=255)                                                                                                                                                                     ggTCAATTTCCGCCGCACTGCGTAACTAGCCGGTCCGTTCGATAAGAAGAACGAACAAAACCAGTACAACA‑GCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCCCCGGGAGATAGc                                                                                                                                     <  1:48916/151‑1 (MQ=255)                                                                                                                                                                     ggTCAATTTCCGCCGCACTGCGTAACNAGCCGGNNCGNTCGATAAGAAGAACGAACANAANNNGNNNANCA‑GCANTGNNAAAAAACGANGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCCCCGNGAGATAGc                                                                                                                                     >  2:48916/1‑151 (MQ=255)                                                                                                                                                                                                    ngTCCGTTCGATAAGAAGAACGAACAAAACCAGTACAAAA‑GCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCCCCGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAGTTCGCCTGATTTTTTATGCAAAAAACGTAAAGATTTTCTACTTCCTTCCTGCAGCAAGCGTAAAGTAAGCAGGCTTATTAtttt  >  1:39508/2‑251 (MQ=255)                                                                                                                                                                                                                     gaaCGNNAANNNCGNNTACAACA‑GCAATGGCAAAAAACGNTGACGTGATAATCAGTGTCTCCAAAAACANTNGNTCGANNATANCATGCACCCGNGAGATAGCTGTTTTCACGTTATTGTTAGCGTGc                                                                                                            >  2:160468/1‑128 (MQ=255)                                                                                                                                                                                                                          |                                                                                                                                                                                                                                      GTCTTCGGCGCGATCCCCCCTTTCAGCTTCCATCCAAAACTCAAACTGGTTGCCGACCCTCTACTCTTCGAACGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAACTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACA‑GCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCCCCGGGAGATAGCTGTTTTCACGTTATTGTTAGCGTGCACAAATGGCAGTTTGATGACAGTTCGCCTGATTTTTTATGCAAAAAACGTAAAGATTTTCTACTTCCTTCCTGCAGCAAGCGTAAAGTAAGCAGGCTTATTATTTT  >  NC_000913/1873889‑1874334

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑