Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,753,721	C→T	G37D (GGC→GAC)	ydhV ←	putative oxidoreductase YdhV

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,753,721	0	C	T	100.0%	30.1 / NA	12	G37D (GGC→GAC)	ydhV	putative oxidoreductase YdhV
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (6/6);  total (6/6)

GCAAAAAAGCCACCCATATGGGCATCGACGACTAAATTTCCTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGCACCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGGTTTCGTGCCTGGCGGTACTTCGTCATACATAATTTTGTAGCCGAAGCCCATGCCACCGACAAAACTTTTAAACTTACTGGAATCTTCGAGGGTAATATTTCCTGTCGTGAGATTGACTCTTAATATATTACCTGTCCAACCGTTAGCCATGATTTTTTCCTTTGCAAGATTCACACAGTAATATCTTTCCACTCGATAATTTTTAACGCCCCTGTTGGGCAGGCGTTTGCGCATTCACCGCATAACACACATTTCGAGGATTTTTTACTTTCGGTATTTACGGTGGC  >  NC_000913/1753532‑1753967                                                                                                                                                                                              |                                                                                                                                                                                                                                                       gCAAAAAAGCCACCCATATGGGCATCGACGACTAAATTTCATTTGGTAAACGTAGAAAGTGAGGTGCTCTTTACGCGCGAACTACAGGGGGCACCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGGTTTCGTGCCTGGCGGTACTTCGTCATACATAATTTTGTAGTCGAAGCCCATGCCACCGACAAAACTTTTAAACTTACTGGAATCTTCGAGGGTAATATTTcc                                                                                                                                                                                           >  1:201371/1‑251 (MQ=255) gCAAAAAAGACGCCCATAGGGGCATCGACGAATAAATTTCCTTTTGGAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGCACCAGATCCAGGTAATGGGCCGGGAGCAAAGACTAATTTATTCGCTTCATCGAAAGGGTTCGTGCCTGGCGGGACTTCGTCATACATAATTTTGTAGTCGAAGCCCATTCCACCGACAAAACTGTTAAACTTACTGGAATCTTCGAGGGTAATATTTcc                                                                                                                                                                                           <  1:190176/251‑1 (MQ=255)            ccccATATGGGCATCGAAGACTAAATTTCCTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGCACCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAACGGTTTCGTGCCTGGCGGTACTTCGTCATACATAATTTTGTAGTCGAAGCCCATGCCACCGACAAAACTTTTAAACTTACTGGAATCTTCGAGGGTAATATTTCCTGTCGTg                                                                                                                                                                                    >  1:62175/2‑247 (MQ=255)                    gggCATCGACGACTAAATTTCCTTTGGTAAAAGTAGACAGTGAGGTGATATTTACGCGAGAACTACAGGGGGCACCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGGTTTCGTGCCTGGCGGTACTTCGTCATACATAATTTTGTAGTCGAAGCCCATGCCACCGACAAAACTTT‑AAACTTACTGGAATCTTCGAGGGTAATATTTCCTGTCGTGAGATTGACTCtt                                                                                                                                                                        >  1:165350/1‑250 (MQ=255)                     ggCATCGACGACTAAATTTCCTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACGACAGGGGGCACCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGGTTTCGTGCCTGGCGGTACTTCGTCATACATAATTTTTTAGTCGAAGCCCATGCCACCGACAAAACTTTTAAACTTACTGGAATCTTCGAGGGTAATATTTc                                                                                                                                                                                            <  1:147018/230‑1 (MQ=255)                                        ccTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGCACCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGGTTTCGTGCCTGGCGGTACTTCGTCATACATAATTTTGTAGTCGAAGCCCATGCCACCGACAAAACTTTTAAACTTACTGGAATCTTCGAGGGTAATATTTCCTGTCGTGAGATTGACTCTTAATATATTACCTGTCCAAcc                                                                                                                                                    >  1:156407/1‑251 (MQ=255)                                              gTAAAAGTAGAAAGTGAGGTGATCTTTACGCGAGAACTACAGGGGGCACCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGGTTTCGTGACTGGCGGTAATTCGTCATACATAATTTTGTAGTCGAAg                                                                                                                                                                                                                                                   >  1:122996/1‑150 (MQ=255)                                                                         aCGCGAGAACTACAGGGGGCACCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGGTTTCGTGCCTGGCGGTACTTCGTCATACATAATTTTGTAGTCGAAGCCCATGCCACCGACAAAACTTTTAAACTTACTGGAATCTTCGAGGGTAATATTTCGTGTCGTGAGATTTACTCTTAATATATTACCGGTCCAACCGTTAGCCATGATTTTTTCCTTTGCAAGATTcac                                                                                                                   <  1:121145/251‑1 (MQ=255)                                                                                         gggCACCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGATTCATCGAAAGGTTTCGTGCCTGGCGGTACTTCGTCATACATAATTTTGTAGTCGAAGCCCATGCCACCGACCAAACTTTTAAACTTACTGGAATCTTCGAGGGTAATATTTCCTGTCGTGAGATT‑ACTCTTAATATATTACCTGTCCAACCGTTAGCCCTGATTTTTTCCTTTGCAAGATTCACACAGTAATATCTTTcc                                                                                                   >  1:142095/1‑250 (MQ=255)                                                                                           gCACCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGGTTTCGTGCCTGGCGGTACTTCGTCATACATAATTTTGTAGTCGAAGCCCATGCCACCGACAAAACTTTTAAACTTACTGGAATCTTCGAGGGTAATATTTCCTGTCGTGAGATTGACTCTTAATATATTACCTGTCCAACCGTTAGCCATGATTTTTTCCTTTGCAAGATTCACACAGTAATATCTTTCCAc                                                                                                 <  1:33018/251‑1 (MQ=255)                                                                                                                                   tATTCGCTTCATCGAAAGGTTTCGTGCCTGGCGGTACTTCGTCATACATAATTTTGTAGTCGAAGCCCATGCCACCGACAAAACTTTTAAACTTACTGGAATCTTCGAGGGTAATATTTCCTGTCGTGAGATTGACTCTTAATATATTACCTGTCCAACCGTTAGCCATGATTTTTTCCTTTGCAAGATTCACACAGTAATATCTTTCCACTCGATAATTTTTAACGCCCCTGTTGGGCAGGCGTTTgcn                                                          <  1:52265/250‑2 (MQ=255)                                                                                                                                                                                          gTAGTCGAAGCCCATGCCACCGACAAAACTTTTAAACTTACTGGAATCTTCGAGGGTAATATTGCCTGTCGTGAGATTGACTCTTAATATATTACCTGTCCAACCGTTAGCCATGATTTTTTCCTTTGCAAGATTCACACAGTAATATCGTTCCACTCGATAATGTTTAACGCCCCTGTTGGGCAGGCGTTTGCGCATTCGCCGCATAACACACATTTCGAGGATTTTTTACTTTCGGTATTTACGGTgac  <  1:193603/251‑3 (MQ=255)                                                                                                                                                                                              |                                                                                                                                                                                                                                                       GCAAAAAAGCCACCCATATGGGCATCGACGACTAAATTTCCTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGCACCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGGTTTCGTGCCTGGCGGTACTTCGTCATACATAATTTTGTAGCCGAAGCCCATGCCACCGACAAAACTTTTAAACTTACTGGAATCTTCGAGGGTAATATTTCCTGTCGTGAGATTGACTCTTAATATATTACCTGTCCAACCGTTAGCCATGATTTTTTCCTTTGCAAGATTCACACAGTAATATCTTTCCACTCGATAATTTTTAACGCCCCTGTTGGGCAGGCGTTTGCGCATTCACCGCATAACACACATTTCGAGGATTTTTTACTTTCGGTATTTACGGTGGC  >  NC_000913/1753532‑1753967

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑