Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,718,435	C→T	intergenic (‑40/+58)	mliC ← / ← anmK	inhibitor of c‑type lysozyme, putative lipoprotein/anhydro‑N‑acetylmuramic acid kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,718,435	0	C	T	100.0%	18.5 / NA	8	intergenic (‑40/+58)	mliC/anmK	inhibitor of c‑type lysozyme, putative lipoprotein/anhydro‑N‑acetylmuramic acid kinase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (6/2);  total (6/2)

GTTTTTCATCACACTGGTATTCCAGCGTATCGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGAAGACTTTCCTGGTCTGGAGGGCAATACGCCCTCCCTAACGTTCCAAGTGTAACGGCAGACGCGGTAAGAAAAATTCAGTTAACTCTGATATCACGGGTTAGCGGGGAAAATAGCCCCCAGTACCGTCTCCTGGCTTGCGCCAGTGACGGAAGGCAGATTTCCTGGTAATCCCGCCAGCGTCCGCCAGGCAAGCCAGGCGAAAGCCAATGCTTCCATGTCATCGCCACTAATGCCAACGGCATCGGTGGTGGTGACTTCTGTGCCTGGCAGTAATGCC  >  NC_000913/1718275‑1718679                                                                                                                                                                 |                                                                                                                                                                                                                                                     gTTTTTCATCACACTGGTATTCCAGCGTATCGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGAAGACTTTCCTGGTCTGGAGGGCAATACGCCCTTCCTAACGTTCCAAGTGTAACGGCAGACGCGGTAAGAAAAATTCAGTTAACTCTGATATCACGGGTTAGCGGGGAAAATAGCACCCAGTAc                                                                                                                                                            >  1:89890/1‑251 (MQ=255)                         ncGTATCGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGAAGACTTTCCTGGTCTGGAGGGCAATACGCCCTTCCTAACGTTCCAAGTGTAACGGCAGACGCGGTAAGAAAAATTCAGTTAACTCTGATATCACGGGTTAGCGGGGAAAATAGCCCCCAGTACCGTCTCCTGGCTTGCGCCAGTGAc                                                                                                                                    >  1:23124/2‑251 (MQ=255)                             aTCGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGAAGACTTTCCTGGTCTGGAGGGCAATACGCCCTTCCTAACGTTCCAAGTGTAACGGCAGACGCTGTAAGAAAAATTCAGTTAACTCTGATATCACGGGTTAGCggg                                                                                                                                                                              <  1:37247/205‑1 (MQ=255)                                                                                               gATAATTAACAGTTTTTTCATAGTCATATCCCGAAGACTTTCCTGGTCTGGAGGGCAATACGCCCTTCCTAACGTTCCAAGTGTAACGNNAGACGCNNt                                                                                                                                                                                                                      >  2:6840/1‑99 (MQ=255)                                                                                               gATAATTAACAGTTTTTTCATAGTCATATCCCGAAGACTTTCCTGGTCTGGAGGGCAATACGCCCTTCCTAACGTTCCAAGTGTAACGGCAGACGCGGTAAGAAAAATTCAGTTAACTCTGATATCACGGGTTAGCGGGGAAAATAGCCCCCAGTACCGTCTCCTGGCTTGCGCCAGTGACGGAAGGCAGATTTCCTGGTAATCTCGCCAGCGTCCGCCAgg                                                                                           <  1:6840/222‑1 (MQ=255)                                                                                                                               gAAGACTTTCCTGGTCTGGAGGGCAATACGCCCTTCCTAACGTTCCAAGTGTAACGGCAGACGCGGTAAGAAAAATTCAGTTAACTCTGATATCACGGGTTAGCGGGGAAAATAGCCCCCAGTACCGTCTCCTGGCTTGCGCCAGTGACGGAAGGCAGATTTCCTGGTAATCTCGCCAGCGTCCGCCAGGCCAGCCAGGc                                                                                 >  1:73817/1‑200 (MQ=255)                                                                                                                                         cTGGTCTGGAGGGCAATACGCCCTTCNTAACGTNNCANGTGTAACGGCAGACGCGGTNAGNNNANNNCNGTTAANTCNNATATCACGGNTTAGCGGGGAAAATAGCCCCCAGTACCGTCTCCTGGCTTGCGCCAGTGACGGANGGCAGATTTNNTGGTAATCTCGCCAGCGTCCGNCAGNNANNCNAgg                                                                                  >  2:51132/1‑189 (MQ=255)                                                                                                                                                           cGCCCTTCCTAACGTTCCAAGTGTAACGGCAGACGTGGCACGAAAAATTCAGTTAACTCTGATATCACGGGTTAGCGGGAAAAATAGACCCCAGTACCGTCTCCTGGCTTGCGCCAGTGACGGAAGGAAGATTTCCTGGTAATCTCGCCAGCGTCCGCCAGGCAAGCCAGGCGAAAGCCAATGCTTCCATGTCATCGCCACTAATGCCAACGGCATCGGAGGTGGTGACTTCTGTGCCTGGCAGTAATgcc  >  1:79405/1‑251 (MQ=255)                                                                                                                                                                 |                                                                                                                                                                                                                                                     GTTTTTCATCACACTGGTATTCCAGCGTATCGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGAAGACTTTCCTGGTCTGGAGGGCAATACGCCCTCCCTAACGTTCCAAGTGTAACGGCAGACGCGGTAAGAAAAATTCAGTTAACTCTGATATCACGGGTTAGCGGGGAAAATAGCCCCCAGTACCGTCTCCTGGCTTGCGCCAGTGACGGAAGGCAGATTTCCTGGTAATCCCGCCAGCGTCCGCCAGGCAAGCCAGGCGAAAGCCAATGCTTCCATGTCATCGCCACTAATGCCAACGGCATCGGTGGTGGTGACTTCTGTGCCTGGCAGTAATGCC  >  NC_000913/1718275‑1718679

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑