Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,703,008	C→T	A259V (GCT→GTT)	add →	adenosine deaminase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,703,008	0	C	T	100.0%	19.9 / NA	9	A259V (GCT→GTT)	add	adenosine deaminase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (5/4);  total (5/4)

CACTTCAACCGCGCGCGTGATGCGGGCTGGCATATTACCGTCCATGCAGGCGAAGCTGCCGGGCCGGAAAGCATCTGGCAGGCGATTCGTGAACTGGGTGCGGAGCGTATTGGACATGGCGTAAAAGCCATTGAAGATCGGGCGCTGATGGATTTTCTCGCCGAGCAACAAATTGGTATTGAATCCTGTCTGACCTCCAATATTCAGACCAGCACCGTAGCAGAGCTGGCTGCACATCCGCTGAAAACGTTCCTTGAGCATGGCATTCGTGCCAGCATTAACACTGACGATCCCGGCGTACAGGGAGTGGATATCATTCACGAATATACCGTTGCCGCGCCAGCTGCTGGGTTATCCCGCGAGCAAATCCGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTG  >  NC_000913/1702779‑1703193                                                                                                                                                                                                                                      |                                                                                                                                                                                          cACTTCAACCGCGCGCGTGATGCGGGCTGGCCTATTACCGTCCATGCAAGCGAAGCTGCCGGGACGGAAAGCATCTGACCGGCGATTCGTGAACTGGGTGCGGAGCGTATTGGACATGGCGTAAAAGACATTGAAGATCGGGCGCTGATGGATTTTCTCGCCGAGCAACAAATTGGTATTGAATCCTGTCTGACCTCCAATATTCAGACCAGCACCGTAGCAGAGCTGGTTGCACATCCGCTGAAAACGtt                                                                                                                                                                      >  1:181154/1‑251 (MQ=255)                                                                 cggAAAGCATCTGGCAGGCGATTCGTGAACTGGGTGCGGAGCGTATTGGACATGGCGTAAAAGCCATTGAAGATCGGGCGCTGATGGATTTTCTCGCCGAGCAACAAATTGGTATTGAATCCTGTCTGACCTCCAATATTCAGACCAGCACCGTAGCAGAGCTGTTTGCACATCCGCTGAAAACGTTCCGTGAGCATTGCATTCGTGCCAGCATTAACACTGACGATCCCGGCGTACAGGGAGTGGATATc                                                                                                      <  1:85591/251‑1 (MQ=255)                                                                                                             aTTGGACATGGCGTAAAAGCCATTGAAGATCGGGCGCTGATGGATTTTCTCGCCGAGCAACAAATTGGTATTGAATCCTGTCTGACCTCCAATATTCAGACCAGCACCGTAGCAGAGCGGGTTGCACATCCGCTGAAAACGTTCCTTGAGCATGGCATTCGTGCCAGCATTAACACTGACGATCCCGGCGTACAGGGAGTGGATATCATTCACGAATATACCGTTGCCGCGCCAGCTGCTGGGTTATccc                                                           <  1:30071/250‑1 (MQ=255)                                                                                                                  aCATGGCGTAAAAGCCATTGAAGATCGGGCGCTGATGGATTTTCTCGCCGAGCAACAAATTGGTATTGAATCCTGTCTGACCTCCAATATTCAGACCAGCACCGTAGCAGAGCTGGTTGCACATCCGCTGAAAACGTTCCTTGAGCATGGCATTCGTGCCATCATTAACACTGACGATCCCGGCGTACAGGGAGTGGATATCATTCACGAATATAcag                                                                                      <  1:200999/218‑3 (MQ=255)                                                                                                                     tGGCGTAAAAGCCATTGACGATCGGGCGCTGACGGATTTTCTCGCCGAGCAACAAATTGGTATTGAATCCTGTCTGACCTACAATATTCAGACCAGCACCGTAGCAGAGCTGGTTGCACATCCGCTGAAAACGTTCCTTGAGCATGGCATTCGTGCCAGCATTAACACTGACGATCCCGGCGTACAGGGAGTGGATATCATTCACGCATATACCGTTGc                                                                                  >  1:186468/1‑219 (MQ=255)                                                                                                                        ngTAAAAGCCATTGAAGATCGGGCGCTGATGGATTTTCTCGCCGAGCAACAAATTGGAATTGAATCCTGTCTGACCTCCAATATTCAGACCAGCACCGTAGCAGAGCTGGTTGCACATCCGCTGCAAACGTTCCTTGAGCATGGCATTCGTGCCAGCATTAACACTGACGATCCCGGCGTACAGGGAGTGGATATCATTCACGAATATACCGTTGCCGCGCCAGCTGCTGGGTTATCCCGCGAGCAACTcc                                               >  1:18691/2‑251 (MQ=255)                                                                                                                                                                      caacaaATTGGTATTGAATCCTGTCTGACCTCCAATATTCAGACCAGCACCGTAGCCGAACTGGTTGCACATCCGCTGAAAACGTTCCTTGAGCATAGCATTCGTGCCAGCATTAACACTGACGCTCCCGGCGTACCGGGAGTGGATATCATTCACGAATATACCGTTGCCGCGCCCGCTGCTGGGTTATCCCGCGAGCAAATCCGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTg  >  1:219145/1‑250 (MQ=255)                                                                                                                                                                      caacaaATTGGTATTGAATCCTGTCTGACCTCCAATATTCAGACCAGCACCGTAGCAGAGCTGGTTGCACATCAGCTGAAAACGTTCCTTGAGCATGGCCTTCGTGCCAGCATTAACACTGACGATCCCGGCGTACAGGGAGTGGATATCATTCACGAATATACCGTTGCCGCGCCAGCTGCTGGGTTATCCCGCGCGCAAATCCGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTg  >  1:219157/1‑250 (MQ=255)                                                                                                                                                                                                                       ccGTAGCATAGCTGGTTGCACATCCGCTGAAAACGTGCCTGGAGCATGTCATTCGTTCCAGCATTAACACTGACGTTCCCGGCGTACAGGGAGTGGATATCATTCACGAATATAcc                                                                                       <  1:30479/116‑1 (MQ=255)                                                                                                                                                                                                                                      |                                                                                                                                                                                          CACTTCAACCGCGCGCGTGATGCGGGCTGGCATATTACCGTCCATGCAGGCGAAGCTGCCGGGCCGGAAAGCATCTGGCAGGCGATTCGTGAACTGGGTGCGGAGCGTATTGGACATGGCGTAAAAGCCATTGAAGATCGGGCGCTGATGGATTTTCTCGCCGAGCAACAAATTGGTATTGAATCCTGTCTGACCTCCAATATTCAGACCAGCACCGTAGCAGAGCTGGCTGCACATCCGCTGAAAACGTTCCTTGAGCATGGCATTCGTGCCAGCATTAACACTGACGATCCCGGCGTACAGGGAGTGGATATCATTCACGAATATACCGTTGCCGCGCCAGCTGCTGGGTTATCCCGCGAGCAAATCCGCCAGGCACAGATTAATGGTCTGGAAATGGCTTTCCTCAGCGCTG  >  NC_000913/1702779‑1703193

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑