Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,700,296	C→T	R314R (CGC→CGT)	malX →	PTS enzyme IIBC component MalX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,700,296	0	C	T	100.0%	11.2 / NA	7	R314R (CGC→CGT)	malX	PTS enzyme IIBC component MalX
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (3/4);  total (3/4)

CGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACGGTTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGTTAGCTATGTATCACTGCGCGCGCCCGGAAAATCGCCATAAAATTAAAGGTCTGCTGATTTCTGGCCTGATCGCCTGCGTCGTTGGCGGCACTACCGAACCGCTGGAATTCCTGTTCCTGTTCGTAGCGCCAGTTCTGTATGTCATCCACGCGCTGTTAACCGGCCTCGGCTTCACCGTCATGTCTGTGCTCGGCGTCACCATCGGTAATAC  >  NC_000913/1700140‑1700484                                                                                                                                                             |                                                                                                                                                                                             cGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACGGTTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGTTAGCTATGTATCACTGCGCGCGTCCGGAAAATCGCCATAAAATTAAAGGTCTGCTGATTTCTGACCTGATCGCCTGCGTCGTTGGCGGCACTACCGAACCGCTGGAAttcctgttcc                                                                                                <  1:32294/251‑1 (MQ=255)              cTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACGGTTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGTTAGCTATGTATCACTGCGCGCGTCCGGAAAATCGCCATAAAATTAAAGGTCTGCTGATTTCTGGCCTGATCGCCTGCGTCGTTGGCGGCACTACCGAACCGCTGGAATTCCTGTTCCTGTTCGTAGCGcc                                                                                   >  1:3309/1‑251 (MQ=255)                                                           ttttCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGTTAGCTATGTATCACTGCGCGCGTCCGGAAAATCGCCATAAAATTAAAGGTCTGCTGATTTCTGGCCTGATCGCCTGCGTCGTTGGCGGCACTACCGAACCGCTGGAATTCCTGTTCCTGTTCGTAg                                                                                       <  1:203781/202‑1 (MQ=255)                                                            tttCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGACGGTCTGCCAGGTGCAGCGTTAGCTATATATCACTGCGCGCGTACGGAAAATCGCCATAAAATTACAGGTCTGCTGATTTCTGGCCTGATCGCCTGCGTCGTTGGCGGCACTACCGAACCGCTGGAATTCCTGTTCCTGTTCGTAGCGCCAGTTCTGTATGTCATCCACGCGCTGTTAACCGGCCTCGGCTTCAcc                                     >  1:102915/1‑251 (MQ=255)                                                                      ncnccnncgTTNCCTTTCGCAAGTTAAAATGCCTGCNTTTCTCGGCGGTCTGCCAGGTGCAGCGTTAGNTNTGTATCACTGCGCGCGTCCGGAAAATCGCCATAAAATTAAAGGTCTGCNGATTTCTGGCNTGANNGCCTNCNTNGTTGGCGGCACTACCGAACCGCTGGAATTNCTGTTCCTGTTCGTAGCGCCAGTNNTGNNNGTNNTCCAc                                                                <  2:164081/207‑1 (MQ=255)                                                                                               aaaaTGCCTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGTTAGCTATGTATCACTGCGCGCGTCCGGAAAAGCGCCATAAAATTAAAGGTCTGCTGATTTCTGGCCTGATCGCCTGCGTCGTTGGCGGCACTGCCGAACCGCTGGAATTCCTGTTCCTGTTCGTAGCGCCAGTTCTGTATGTCATCCACGCGCTGTTAACCGGCCTCGGCTTCACCGTCATGTCTGTGCTCGGCGTCACCATCGGTAATAc  <  1:84773/251‑1 (MQ=255)                                                                                                                            nggTGCAGCGTTAGCTATGTATCACTGCGCGCGTCCGGAAAATCGCCATAAAATTAAAGGTCTGCTGATTTCTGGCCTGATCGCCTGCGTCGTTGGCGGCACTACCGAACCGCTGGAAttcctgttcc                                                                                                >  1:44941/2‑128 (MQ=255)                                                                                                                                                             |                                                                                                                                                                                             CGTCAGCGGCGCACTGACCATCTTCCAGGCGCAATTGAGTTGCCCGACCACTCACGGTTTTTCTGAAAGCGCCACGCGTTTCCTTTCGCAAGGTAAAATGCCTGCGTTTCTCGGCGGTCTGCCAGGTGCAGCGTTAGCTATGTATCACTGCGCGCGCCCGGAAAATCGCCATAAAATTAAAGGTCTGCTGATTTCTGGCCTGATCGCCTGCGTCGTTGGCGGCACTACCGAACCGCTGGAATTCCTGTTCCTGTTCGTAGCGCCAGTTCTGTATGTCATCCACGCGCTGTTAACCGGCCTCGGCTTCACCGTCATGTCTGTGCTCGGCGTCACCATCGGTAATAC  >  NC_000913/1700140‑1700484

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑