Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,697,394 C→T R216K (AGA→AAA)  hdhA ← 7‑alpha‑hydroxysteroid dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,697,3940CT92.9% 35.3 / NA 14R216K (AGA→AAA) hdhA7‑alpha‑hydroxysteroid dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (9/4);  minor base G (0/1);  total (9/5)
Fisher's exact test for biased strand distribution p-value = 3.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGTTAACCAATAGCCGTGATTTGGCTGCCAGTTATTTAGCAGAGACGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCTTGCGGTTGGCCCAGACGTCTGATCGGCGTGTGCTGTAACATTTTTTGTTCAATTTCTGGTGTAATAACGGATTTCAGGGCATCGGTTAATATTGCCCCCGGCGCAATGCCATTTACCCGAATATTTTTTTCACCCAGGTCAAACGCCATATTTCTGACCAGATGACTGGCCGCAGCTTTAGATGATGCATAGGAAGTCATGTTTATATTTTTATTTTCTGCCGCCATAGAAGTGATGGTCAGAATAACGC  >  NC_000913/1697169‑1697625
                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                       
ggTTAACCAATAGCCGTGATTTGGCTGCCCGTTATTTAGCAGAGACGTTGATGAGGTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGCGCAAAGGAACAGCGCTGCGTTAGCAATATCTTGCGGTTGGCCCAGACGTTTGATCGGCGTGTGATGTAACAtttt                                                                                                                                                                                                                >  1:191995/1‑251 (MQ=255)
                                                             ccGGTAAACAACACGCACGGTTGTTTNCCGGTCNNTTNGTGTATTAATTGAGCTCCTNTANNNCNNNANCGGAGNCGNNGAGAATTTGNCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATNTCTTGCGGTNNGCCCAGACGTTTGATCGGCGTNTGGNNTNNANtttntt                                                                                                                                                                                                              >  2:47033/1‑192 (MQ=255)
                                                                           ncacgGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCTTGCGGTTGGCCCAGACGTTTGATCGGCGTGTGCTGTAACATTTTTTg                                                                                                                                                                                                             >  1:131760/2‑179 (MQ=255)
                                                                                                       aTTAATTGAGCTCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCTTGCGGTTGGCCCAGACGTTTGATCGGCGTGTGCTGTAACATTTTTTGTTCAATTTCTGGTGTAATAACGGATTTCAGGTCATCGGTTAATATTGCCCCCGGCGCAATGCCATTTACCCGAATATTTTTTTCACCCAGGTCAAACGc                                                                                                          <  1:180715/250‑1 (MQ=255)
                                                                                                              gANNNNNTGTNCCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCTTGCGGTTGGCCCAGACGTTTGATCGGCGTGTGCTGTAACATTTTTTg                                                                                                                                                                                                             <  2:131760/144‑1 (MQ=255)
                                                                                                                gCTCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCTTGCGGTTGGCCCAGACGTGTGATCGGCGTGTGCTGTAACATTTTTTGTTCAATTTCTGGTGTAATAACGGATTTCATGGCATCGGTTAATAGTGCCCCCGGAGCAATGCCATTTTCCCGAATATTTTGTTCACCCAGGTCAAACGCCATATTTCTg                                                                                                <  1:81838/251‑1 (MQ=255)
                                                                                                                   ccTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCTTGCGGTTGGCCCAGACGTTTGATCGGCGTGTGCTGTAACATTTTTTGTTCAATTTCTGGTGTAATAACGGATTTCAGGGCATCGGTTAATATTGc                                                                                                                                                             >  1:150720/1‑187 (MQ=255)
                                                                                                                   ccTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCGTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCTTGCGGTNGGCNCAGANGTNTGATCGGCGTGTGCTNTAACATTTTTTGTTCAATTTCTGGTGTAATAACGGATTTCAGGGNNTNNNTNAATATTGc                                                                                                                                                             <  2:150720/187‑1 (MQ=255)
                                                                                                                                         ggTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCTTGCGGTTGGCCCAGACGTTTGATCGGCGTGTGCTGTAACATTTTTTGTTCAATTTCGGGTGTAATAACGGATTTCAGGGCATCGGTTAATATTGCCCCCGGCGCAATGCCATTTACCCGAATATTTTTTGCACCCAGGTCAAACGCCATATTTCTGACCAGATGACTGGCCGCAGCTTTAg                                                                       <  1:35036/251‑1 (MQ=255)
                                                                                                                                              naaTTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCTTGCGGTTGGCCCAGACGTTTGATCGGCGTGTGCTGTAACATTTTTTGTTCCATTTCTGGTGTAATAACGGATTTCAGGGCCTCGGTTAATATTGCCCCCGGCGCAATGCCAttt                                                                                                                                          >  1:19147/2‑179 (MQ=255)
                                                                                                                                                               cAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCTTGCGGTTGGCCCAGACGTTTGATCGGCGTGTGCTGTAACATTTTTTGTTCAATTTCTGGTGTAATAACGGATTTCAGGGCATCGGTTAATATTGCCCCCGGCGCAATGCCATTTACCCGAATATTTTTTTCACCCAGGTCAAACGCCATATTTCTGACCAGATGACTGGCCGCAGCTTTAGATGATGCATTGGAAGTCATGtt                                                 >  1:35961/1‑251 (MQ=255)
                                                                                                                                                               cAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCTTGCGGTTGGCCCAGACGTTTGATCGGCGTGCGCTGTAACATTTTTTGTTCAATTTCTGGTGTAATAACGGATTTCCGGGCATCGGTTAATATTGTCCCCGGCGCAATGCCATTTTCCCGCATATTTTTTTCACCCAGGTCAAACGCCATATTTCTGACCAGATGACTGGCCGCAGCTTTAGATGATGCATAGGAAGACATGtt                                                 >  1:35967/1‑251 (MQ=255)
                                                                                                                                                                           ggCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCTTGCGGTTGGCCCAGACGTTTGATCGGCGTGTGCTGTAACCTTTTTTGTTCAATTTCTGGTGTAATAACGGATTTCAGGGCATCGGTTAATATTTCCCCCGGCGCACTGCCATTTACCCGAATATTTTTTTCACCCAGGTCAAACGCCATATTTCTGACCAGATGACTGGCCGCAGCTTTAGATGCTGCCTAGGCAGTCATGTTtttatttttatt                                     >  1:125081/1‑251 (MQ=255)
                                                                                                                                                                                                               tGCGGTTGGCCCAGACGTTTGATCGGCGTGTGCTGTAACATTTTTTGTTCAATTTCTGGTGTAATAACGGATTTCAGGGCATCGGTTAATATTGCCCCCGGCGCAATGCCATTTACCCGAATATTTTTTTCACCCAGGTCAAACGCCATATTTCTGACCAGATGACTGGCCGCAGCTTTAGATGATGCATAGGAAGTCATGTTTATATTTTTATTTTCTGCCGCCATAGAAGTGATGGTGAGAATAACGc  <  1:47033/250‑1 (MQ=255)
                                                                                                                                                                                                                       gCCCAGACGTTTGATCGGCGTGTGCTGTAACCTTTTTTGTTCAATTTCTGGTGTAATAACGGATTTCAGGGCATCGGTTAATATTGCCCCCGGCGCCATGCCATTTACCCGAATATTTTTTTTACCCAGGTCAAACGCCATATTTCTGACCAGCTGACTGGCCGCAGCTTTAGATGATGCATAGGAAGTCATGTTTTtattttt                                        >  1:9384/1‑204 (MQ=255)
                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                       
GGTTAACCAATAGCCGTGATTTGGCTGCCAGTTATTTAGCAGAGACGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCTTGCGGTTGGCCCAGACGTCTGATCGGCGTGTGCTGTAACATTTTTTGTTCAATTTCTGGTGTAATAACGGATTTCAGGGCATCGGTTAATATTGCCCCCGGCGCAATGCCATTTACCCGAATATTTTTTTCACCCAGGTCAAACGCCATATTTCTGACCAGATGACTGGCCGCAGCTTTAGATGATGCATAGGAAGTCATGTTTATATTTTTATTTTCTGCCGCCATAGAAGTGATGGTCAGAATAACGC  >  NC_000913/1697169‑1697625

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: