Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,676,399	C→T	R502H (CGC→CAC)	pntA ←	pyridine nucleotide transhydrogenase subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,676,399	0	C	T	100.0%	30.9 / NA	12	R502H (CGC→CAC)	pntA	pyridine nucleotide transhydrogenase subunit alpha
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (5/7);  total (5/7)

GATCCAGCCAACATTACCCGTATCCGGTCCAAAAATGGTTGCGATTAACGCAATCGCCATCCCGGCGATACCGAAGTTGTTACCCTGGCGAGACGTTTCATGTTTCGAAAGACCGGCCAGACTGAAGATAAACAGGATCGCGGCAACAATGTATGCAGCTGTAACTAATCCTCCAGACATATGTTACCCCTTAATTTTTGCGGAACATTTTCAGCATGCGCTGAGTCACGGTGAAGCCACCGAAAATATTAATGCTGGCTATAAGCACCGCGATAAAACTAAGGAAGCTAACCCAGCCGCCCTGGCCAATCTGCAACAGTGCTCCGACAACAATAATCCCTGAAATCGCGTTGGTGACCGACATCAACGGTGTATGCAG  >  NC_000913/1676181‑1676559                                                                                                                                                                                                                           |                                                                                                                                                                 naTCCAGCCAACATTACCCGTATCCGGTCCAAAAATGGTTGCGATTAACGCAATCGCCATCCCGGCGATACCGAAGTTGTTACCCTGGCGAGACGTTTCATGTTTCGAAAGACCGGCCAGACTGAAGATAAACAGGATCGCGGCAACAATGTATGCAGCTGTAACTAATCCTCCAGACATATGTTACCCCTTAATTTTTGCGGAACATTTTCAGCATGTGCTGAGTCACGGTGAAGCCACCGAAAATAtt                                                                                                                                   >  1:132657/2‑250 (MQ=255)                                      gTTGCGATTAACGCAATCGCCATCCCGGCGATACCGAAGTTGTTACCCTGGCGAGACGTTTCATGTTTCGAAAGACCGGCCAGAATGAAGATCAACAGGATCGCGGCAACAATGTATGCAGCTGTAACTAATCCTCCAGACATATGTTACCCCTTAATTTTTGCGGCACATCTTCAGCATGTGCTGAGTCACGGTGAAGCCACCGAAACTATTAATGCTGGCTATAAGCACCGCGATAAAACTAAGGAAGc                                                                                             >  1:105811/1‑251 (MQ=255)                                                   cAATCGCCATCCCGGCGATACCGAAGTTGTTACCCTGGCGAGACGTTTCATGTTTCGAAAGACCGGCCAGACGGAAGATAAACAGGATCGCGGCAACAATGTAGGCAGCTGTAACTAATCCTCCAGACATATGTTCCCCCTTAATTTTTGCGGAACATTTTCAGCATGTGCTGAGTCACGGTGAAGCCACCGAAAATATTAATGCTGGCTATAAGCACCGCGATAAAACTAAGGAAGCTAACCCAgccgcc                                                                                <  1:75968/251‑1 (MQ=255)                                                                               gtttcccTGGCGAGACGTTTCATGTTTCGAAAGACCGGCCAGACTGAAGATAAACAGGATCGCGGCAACAATGTATGCAGCTGTAACTAATCCTCCAGACATATGTTACCCCTTAATTTTTGCGGAACATTGTCAGCATGTGCTGAGTCACGGTGAAGCCACCGAAAATATTAATGCTGGCTATAAGCACCGCGATAAAACTAAg                                                                                                  <  1:81410/201‑1 (MQ=255)                                                                               gttACCCTGGCGAGACGTTTCATGTTTCGAAAGACCGGCCAGACTGAAGATAAACAGGATCGCGGCAACAATGTATGCAGCTGTAACTAATCCTCCAGACATATGTTACTCCTTAATTTTTGCGGAACATTTTCAGCATGTGCTGAGTCACGGTGAAGCCACCGAAAATATTAATGCTGGCTATAAGCACCGCGATAAAACTAAGGAATCTAACCCAGCCGCCCTGGCCAATCTGCAACAGTGCTCCGac                                                     <  1:98036/250‑1 (MQ=255)                                                                                                         tCGAAAGACCGGCCAGACTGAAGATAAACAGGATCGCGGCAACAATGTATGCAGCTGTAACTAATCCTCCAGACATATGTTAGCCCTTAATTTTTGCGGAACATTTTCAGCATGTGCTGAGGCACGGTGTAGCCACGGAAAATATTAATGCTGGCTATAAGCACCGCGATAAAACTAAGGAAGCTAACCCAGCCGCCCTGGCCAATCTGCAACAGTGCTCCGACAACAATAATCCCTGAAATCGCGTTgg                           <  1:126844/250‑1 (MQ=255)                                                                                                             aaGACCGGCCAGACTGAAGATAAACAGGATCGCGGCAACAATGTATGCAGCTGTAACTAATCCTCCAGACATATGTTACCCCTTAATTTTTGCGGAACATTGTCAGCATGTGCTGAGTCACGGTGAAGCCACCGAAAATATTAATGCTGGCTATAAGCACCGCGATAAAACTAAGGAAGCTAACCCAGCCGCCCTGGCCAATCTGCAACAGTGCTCCGACAACAATAATCCCTGAAATCGCGTTGGTGAcn                      <  1:25522/251‑2 (MQ=255)                                                                                                                                       ggATCGCGGCGACAATGTATGCAGCTGTAACGAATCCTCCAGACATATGTTACCCCTTAATTTGTGCGGAACATTTTCAGCATGTGCTGAGTCACGGTGAAGCCACCGAAAATATTAATGCTGGCTATAAGCACCGCGATAAAACTAAGGAAGCTAAcc                                                                                        <  1:216844/159‑1 (MQ=255)                                                                                                                                         aTCGCGGCAACAATGTATGCAGCTGTAACTAATCCTCCAGACATATGTTACCACTTACTTTTTGCGGAACATTTTCAGCATGTGCTGAGTCAAGGTGaa                                                                                                                                                  >  1:114173/1‑99 (MQ=255)                                                                                                                                               gCAACAATGTATGCAGCTGTAACTAATCCTCCAGACATATGTTACCCCTTAATTTTTGCGGAACATTTTCAGCATGTGCTGAGTCACGGTGAAGCCACCGAAAATATTAATGCTGGCTATAAGCACCGCGATAAAACTAAGGAAGCTAACCCAGCCGCCCTGGCCAATCTGCAACAGTGCTCCGACAACAATAATCCCTGAAATCGCGTTGGTGACCGACATCAACGgtg         <  1:1261/230‑1 (MQ=255)                                                                                                                                                                                                  aTTTTTGCGGAACATTTTCAGCATGTGCTGAGTCACGATCAAGCCACCGAACATATTAATGATAGATCTAAGCACCGCGATAAAACTAAGGAAGCTAACACAGCAGCCATGACCAATATGCCACAGTGCTCCGACAACAATAATCCCTGCAATCGCGTTGGTGACCGACATCAACGGTGTATGCAg  >  1:118566/1‑186 (MQ=255)                                                                                                                                                                                                        gCGGAACATTTTCCGCATGTGCTGAGTCACGGTGAAGCCACCGAAAATATTAATGCTGGCTATAAGCACCGCGATAAAACTAAGGAAGCTAACCCAGCCGCCCTGGCCAATCTGCAACAGTGCTCCGACAACAATAATCCCTGAAATCGCGTTGGTGACCGACATCAACGGTGTAt      >  1:68958/1‑176 (MQ=255)                                                                                                                                                                                                                           |                                                                                                                                                                 GATCCAGCCAACATTACCCGTATCCGGTCCAAAAATGGTTGCGATTAACGCAATCGCCATCCCGGCGATACCGAAGTTGTTACCCTGGCGAGACGTTTCATGTTTCGAAAGACCGGCCAGACTGAAGATAAACAGGATCGCGGCAACAATGTATGCAGCTGTAACTAATCCTCCAGACATATGTTACCCCTTAATTTTTGCGGAACATTTTCAGCATGCGCTGAGTCACGGTGAAGCCACCGAAAATATTAATGCTGGCTATAAGCACCGCGATAAAACTAAGGAAGCTAACCCAGCCGCCCTGGCCAATCTGCAACAGTGCTCCGACAACAATAATCCCTGAAATCGCGTTGGTGACCGACATCAACGGTGTATGCAG  >  NC_000913/1676181‑1676559

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑