Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,673,177	C→T	A109T (GCG→ACG)	mdtJ ←	multidrug/spermidine efflux pump membrane subunit MdtJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,673,177	0	C	T	100.0%	23.2 / NA	10	A109T (GCG→ACG)	mdtJ	multidrug/spermidine efflux pump membrane subunit MdtJ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (7/3);  total (7/3)

CCTTTAACGGCTTGAGAAAGCGCACTAAAGGCAGCCAGCACCGCCGCCAGGGAGAGCAAGCCAAATATTTTGCGACGAAAGCCGTCAGAAAATTTCAAAAAGACGTTAGCAACGATTTCCAGCACGATTGCCAATGCCAGCCAGGCGGCGTGAACCCATTCAAACTGCGCCATGGTTCACCTCCAGTTCAGGTTTACGCGCTTTACGGGTACCTGATTTTATCAACACAATCCCGGCGACCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCGATACCTTCCCACAGCGCATAAGCTACGCCTAAGGCGATTTTTTTAACGGCGAAAGAGAGAAATATATACGACAGAGAAATCATCACCAGCATTAAAATAAAGCC  >  NC_000913/1672977‑1673405                                                                                                                                                                                                         |                                                                                                                                                                                                                                     ccTTTAACGGCTTGAGAAAGCGCACTAAAGGCAGCCAGCACCGCCGCCAGGGGGAGCAAGCCAAATATTTTGCGACGAAAGCCGTCAGAAAATTTCAAAAAGACGTTAGCAACGATTTCCAGCACGATTGCCAATGCCAGCCAGGCGGCGTGAACCCATTCAAACTGCGCCATGGTTCACCTCCAGTTCAGGTTTACGCGTTTTACGGGTACCTGATTTTATCAACACAATCCCGGCGACCAGGGTGGtt                                                                                                                                                                                     >  1:144406/1‑250 (MQ=255)                                                           aGCCAAATATTTTGCGACGAAAGCCGTCAGAAAATTTCACAAAGACGTTAGACACGATTTCCAGCACGCTTCCCAATGCAAGCCAGGCGGCGTGAACCCATTCAAACTGCGCCATGGTTCACCTACAGTTCAGGTTTACGCGTTTTACGGGTACCTGATTTTATCAACACAATCCCGGCGACCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGt                                                                                                                          >  1:125554/1‑251 (MQ=255)                                                                         ngacgaAAGCCGTCAGAAAATTTCAAAAAGACGTTAGCAACGATTTCCAGCACGATTGCCAATGCCAGCCAGGCGGCGTGAACCCATTCAAACTGCGCCATGGTTCACCTCCAGTTCAGGTTTACGCGTTTTACGGGTACCTGATTTTATCAACACAATCCCGGCGACCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAAc                                                                                                                                      >  1:42771/2‑225 (MQ=255)                                                                         ngacgaAAGCCGTCAGAAAATTTCAAAAAGACGTTAGCAACGATTTCCAGCACGATCGCCAATGCCAGCCAGGCGGCGTGAACCCATTCAAACTGCGCCATGGTTCACCTCCAGTTCAGGTTTACGCGTTTTACGGGTACCTGATTTTATCAACACAATCCCGGCGACCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAAc                                                                                                                                                         >  1:38357/2‑206 (MQ=255)                                                                           acgaAAGCCGTCAGAAAATTTCAAAAAGACGTTAGCAACGATTTCCAGCACGATTGCCAATGCCAGCCAGGCGGCGTGAACCCATTCAAACTGCGCCATGGTTCACCTCCAGTTCAGGTTTACGCGTTTTACGGGTACCTGATTTTATCAACACAATCCCGGCGACCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCg                                                                                                          <  1:111673/251‑1 (MQ=255)                                                                            nnnnnnnnnncANNNNNTTTNAAAAAGACGTTAGCAACGATTTCCAGCACGATTGCCAATGCCAGCCAGGCGGCGTGAACCCATTCAAACTGCGCCATGGTTCACCTCCAGTTCAGGTTTACGCGTTTTACGGGTACCTGATTTTATCAAcaca                                                                                                                                                                                                          <  2:133177/144‑1 (MQ=255)                                                                                                             gCAACGATTTCCAGCACGATTGCCAATGCCAGCCAGGCGGCGTGAACCCATTCAAACTGCGCCATGGTTCACCTCCAGTTCAGGTTTACGCGTTTTACGGGTACCTGATTGTATCAACACAATCCCGTCGACCAGGGTGGTTAACCCGGCAATTTGCATCATCGATAAATTTTCGTCGAATAACAAAACGCGAAACAAGGTAATAAATAAAATACCGATACCTTCCCACAGCGCATAAGCTACGCCTAAgg                                                                        <  1:121402/251‑1 (MQ=255)                                                                                                                                                             cATTCAAACTGCGCCATGGTTCACCTCCAGTTCAGGTTTACGCGTTTTACGGGTACCTGATTTTATCAACACAATCCCGGCGACCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCGATACCTTCCCACAg                                                                                            >  1:17374/1‑183 (MQ=255)                                                                                                                                                                               gTTCACCTCCAGTTCAGGTTTACGCGTTTTACGGGTAACTGATTTTATCAACACAATCCCGGCGACCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCGATACCTTCCCACAGCGCATAAGCTACGCCTAAGGCGATTTTTTTAACGGCGAAAGAGAGAAATATATACGACAGAGAAATCATCACCAGCATTAAAATa       >  1:90697/1‑250 (MQ=255)                                                                                                                                                                                   aCCTCCAGTTCAGGGTTACGCGTTTTACGGGTACCTGATTTTATCAACCCAATACCGGCGACCAGGGGGGTTAACACGGCCATTTTCATCAGCGATAAACTTTCGTCGATTAACAAAACGCTAAACACGGTAGTACATAAAATACCGCTACCTTCCCACCGCGCATAAGCTACGCCTAAGGCGATTTTTTTAACGGCGAAAGAGAGAAATATATACGACCGAGAAATAATAACCAGCATTACAATCAAgcc  >  1:126165/1‑251 (MQ=255)                                                                                                                                                                                                         |                                                                                                                                                                                                                                     CCTTTAACGGCTTGAGAAAGCGCACTAAAGGCAGCCAGCACCGCCGCCAGGGAGAGCAAGCCAAATATTTTGCGACGAAAGCCGTCAGAAAATTTCAAAAAGACGTTAGCAACGATTTCCAGCACGATTGCCAATGCCAGCCAGGCGGCGTGAACCCATTCAAACTGCGCCATGGTTCACCTCCAGTTCAGGTTTACGCGCTTTACGGGTACCTGATTTTATCAACACAATCCCGGCGACCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCGATACCTTCCCACAGCGCATAAGCTACGCCTAAGGCGATTTTTTTAACGGCGAAAGAGAGAAATATATACGACAGAGAAATCATCACCAGCATTAAAATAAAGCC  >  NC_000913/1672977‑1673405

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑