Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,672,907	C→T	W81* (TGG→TGA)	mdtI ←	multidrug/spermidine efflux pump membrane subunit MdtI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,672,907	0	C	T	100.0%	12.0 / NA	6	W81* (TGG→TGA)	mdtI	multidrug/spermidine efflux pump membrane subunit MdtI
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (4/2);  total (4/2)

TAATTGGGGTGCAAAGTTCGGCTCCTGCCGCGAAAGATCGCTGGCGCGCCGATAACCGGGCCATTTCTGTTACCGGTTTTCGCGACAAGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCAGGCCAATCCAGCCTTTACGATTTAACCGTTGACCAAACAAGATCCAACCTGCGGCTAACGTGGCGGCAATACCAAACCCGCCCCACAATGCATAAGCGACAGACAAGTCGATCCCTTTAACGGCTTGAGAAAGCGCACTAAAGGCAGCCAGCACCGCCGCCAGGGAGAGCAAGCCAAATATTT  >  NC_000913/1672669‑1673046                                                                                                                                                                                                                                               |                                                                                                                                            taattGGTGTGCAAAGGTCGGCTCCTGCCGCGAAAGATCGCTGGCGCGCCGATAACCGGGCCATTTCTGTTACCGGTTTTCGCGACAAGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATTATCATTCCAGCCAACAGCAAGACCAGGCCAATCCAGCCTTTTCGATTTAACCGTTGACCAAACAAGATTCAACCTGCGGc                                                                                                                                  <  1:204854/250‑1 (MQ=255)                                                                                                 aaCTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCAGGCCAATCCAGCCTTTACGATTTAACCGTTGACCAAACAAGATTCAACCTGCGGCTAACGTGGCGGCAATACCAAACCCGCCCCACAATGCATAAGCGACAGACAAGTCGATCCCTTTAACGGCTTGAGAAAGCGCACTAAAGGcagccagc                                 >  1:6030/1‑251 (MQ=255)                                                                                                                       aaaccctcccagACAGCGCGGGCAGCTGCTACATCAGGCAAGTTTCCCCATTACCATCCCACCCAACAGCAAGACCAGGCCAATCCAACCTTTACGATTTAACCGTTGACCAAACAAGATTCATCCTGCGGCTAACGTGGCGTCAATACCAAACCCTCCCCACAATGCATAAGCTACAGACAAGTCGACCCCTTTAACTGCTTGAGAAAGCTCACTAAAGGCATCCAGCACCGCCGCCAGGGAGAGCAAGc           <  1:29275/240‑1 (MQ=255)                                                                                                                                ccGACAGCGCGGGCAGCGTCTTCATCAGGCACGTTTCACCATGATCATTCCAGCCAACAGCAAGACCAGGCCAATCCAGCCTTTACGATTTAACCGTTGACCAAACAAGATTCAACCTGCGGCTAACGTGGCGGCAATACCAAACCCGCCCCACAATGCATAAGCGACAGACAAGTCGATCCCTTTAACGGCTTGAGAAAGCGCACTAAAGGCAGCCAGCACCGCCGCCAGGGAGAGCAAGCCAAATAttt  >  1:172341/1‑251 (MQ=255)                                                                                                                                           ggCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCAGGCCAATCCAGCCTTTACGATTTAACCGTTGACCAAACAAGATTCAACCTGCGGCTAACGTGGCGGCAATACCAAACCCGCCCCACAATGCATAAGCGACAGACAAGTCGATCCCTTTAACGGCTTGAGAAAgcgc                                                 >  1:61165/1‑193 (MQ=255)                                                                                                                                                          aGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCAGGCCAATCCAGCCTTTACGATTTAACCGTTGACCAAACAAGATTCAACCTGCGGCTAACGTGGCGGCAATACCAAACCCGCCCCACAATGc                                                                                              >  1:73414/1‑133 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                            TAATTGGGGTGCAAAGTTCGGCTCCTGCCGCGAAAGATCGCTGGCGCGCCGATAACCGGGCCATTTCTGTTACCGGTTTTCGCGACAAGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCAGGCCAATCCAGCCTTTACGATTTAACCGTTGACCAAACAAGATCCAACCTGCGGCTAACGTGGCGGCAATACCAAACCCGCCCCACAATGCATAAGCGACAGACAAGTCGATCCCTTTAACGGCTTGAGAAAGCGCACTAAAGGCAGCCAGCACCGCCGCCAGGGAGAGCAAGCCAAATATTT  >  NC_000913/1672669‑1673046

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑