Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,643,094	C→T	A50T (GCC→ACC)	ydfT ←	putative antitermination protein Q

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,643,094	0	C	T	100.0%	30.4 / NA	13	A50T (GCC→ACC)	ydfT	putative antitermination protein Q
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (8/5);  total (8/5)

ACCACTTCGCGCAATACATTATAAAATCCCGTACCTGAACAATGCTCACAGCGAGCCTTACTTGCCGCAGACCTGGAGTAATCAGCAAAGGCAAAACTCACGAGGTAAGGAATAATCTGTAACCGGATTTCTTCACTCAATTTGTTCAATGTCGGGTTATCCAGTGCCATCGCGTAATTTAGCAGGCCTTCAATCGCAAACTGAGGGTCCTGAACACCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAAACAACCGGTGCCTGTCGCTGGAGCGTCATCGCTCAATTTTGGAGATTTTGGGGAGTAATATTTTGGTAAGGCTTCAAGGTTCATGCTCGTTCTCCACTTACGCCAGTACG  >  NC_000913/1642856‑1643267                                                                                                                                                                                                                                               |                                                                                                                                                                              accacTTCGCGCAATACATTATAAAATCCCGTACCTGAACAATGCTCACAGCGAGCCTTACTTGCCGCAGACCTGGAGTAATCAGCAAAGGCAAAACTCACGAGGTAAGGAATAATCTGTAACCGGATTTCTTCACTCAATTTGTTCATTGTCGGGTTATCCAGTGCCATCGCGTAATTTAGCAGGCCTTCAATCGCAAACTGAGGGTCCTGAACACCAACTTTTGCCAGAAATAAGGTCAACCCAAgtgg                                                                                                                                                                   >  1:217450/1‑251 (MQ=255)       tCGCGCAATACATTATAAAATCCCGTACCTGAACAATGCTCACAGCGAGCATTCCTTGCCGCAGACCTGGAGTAATCAGCAAAGGCAAAACTCACGAGGTAAGGAATAATCTGTAACCGGATTTCTTCACTCAATTTGTTCAATGTCGGGTTATCCAGTGCCATCGCGTAATTTAGCAGGCCTTCAATCGCAACCTGAGGGTCCTGAACCCCAACTTTTGCCAGAAATAAGGTCAACCCAAGTGGTGCttt                                                                                                                                                             >  1:94614/1‑251 (MQ=255)             aaTACATTATAAAATCCCGTACCTGAACAATGCTCACAGCGAGCCTTACTTGCCGCAGACCTGGAGTAATCAGCAAAGGCAAAACTCACGAGGTAAGGAATAATCTGTAACCGGATTTCTTCACTCAATTTGTTCAATGTCGGGTTATCCAGTGCCATCGCGTAATTTAGCAGGCCTTCAATCGCAAACTGAGGGTCCTGAACACCAACTTTTGCCAGAAATAAGGTCAACCCAAGTGGTGCTTTCGACTg                                                                                                                                                       <  1:48457/251‑1 (MQ=255)                                                          ttACTTGCCGCAGACCTGGAGTAATCAGCAAAGGCAACACTCACGAGGTACGAAATAATCTGTAACCGCATTTCTTACCTCAATTTGTTCAATGCCGGGTTATCCAGTGCCATCGCGTAATTTAGCAGGCCTTCAATCGCAAACTGAGGGTCCTGAACACCAACTTTTTCCAGAAATAAGGTCAACCCAAGTGGTGCTTTCGACTGCCCCATCCCCTGCGCTGCCATTCCATCCGTAATTGTTAAACAAcc                                                                                                          >  1:102846/1‑251 (MQ=255)                                                                 ccGCAGACCTGGAGTAATAAGCAAAGGCAAAACTCACGAGGAAAGGAATAATCTGTAACCGGATTTCTTCACTCAATTTGTTCAATGTCGGGTTATCCAGTGCCATCGCGTAATTTAGCAGGCCTTCAATCGCAAACTGAGGGTCCTGAACACCAACTTTTGCCAGAAATAAGGTCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAAACAACCGGTGCCt                                                                                                   >  1:186457/1‑251 (MQ=255)                                                                                                        ggTAAGGAATAATCTGTAACCGGATTTCTTCACGCAATTTGTTCAATGTCGGGTTATCCAGTGCCATCGCGTAATTTAGCAGGCCTTCAATCGCAAACTGAGGGTCCTGAACACCAACTTTTGCCAGAAATAAGGTCAACCCAAGTGGTGCGTTCGACTGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAAACAACCGGTGCCTGTCGCTGGAGCGTCATCGCTCAATTTTGGAGATTTTggn                                                            <  1:39095/251‑2 (MQ=255)                                                                                                                                     ncACTCAATTTGTTCAATGTCGGGTTATCCAGTGCCATCGCGTAATTTAGCAGGCCTTCAATCGCAAACTGAGGGTCCTGAACACCAACTTTTGCCAGAAATAAGGTCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAAACAACCGGTGCCTGTCGCTGGAGCGTCATCGCTCAATTTTgg                                                                      >  1:135733/2‑212 (MQ=255)                                                                                                                                          cAATTTGTTCAATGTCGGGTTATCCAGTGCCATCGCGTAATTTAGCAGGCCTTCAATCGCAAACTGAGGGTCCTGAACACCAACTTTTGCCAGAAATAAGGTCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAAACAACCGGTGCCTGTCGCTGGAGCGTCATCGCTCAATTTTGGAGATTTTGGGGAGTAATATTTTGGTAAGGCTTCAAGGTTCATGc                          >  1:102458/1‑251 (MQ=255)                                                                                                                                                                                                 aTCGCAAACTGAGGGTCCGGAACACCAACTTTTGCCAGAAATAAGGTCATCCCAAGTGGTGCTTTCGACTGCACCATc                                                                                                                                                <  1:71915/78‑1 (MQ=255)                                                                                                                                                                                                         cTNNNNNTCCNGAACACCAACTTTTGCCAGAAATAAGGTCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAAACAACCGGTGCCTGTCGCTGGAGCGTCAGCGCTCAATTTTgg                                                                      <  2:135733/144‑1 (MQ=255)                                                                                                                                                                                                                                  nccAGAAATAAGGTCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAAACAACCGGTGCCTGTCGCTGGAGCGTCATCGCTCAATTTTGGAGATTTTGGGGAGTAATATTTTGGTAAGGCTTCAAGGTTCATGCTCGTTCTCCACTTACGCCAGTACg  >  1:135352/2‑187 (MQ=255)                                                                                                                                                                                                                                   cnAGNAATAANGTCANCCCAAGTGGTGCTTTNGACTACCCCATCCCCTGCGNNGNCATTANATCCg                                                                                                                          <  2:94614/66‑1 (MQ=255)                                                                                                                                                                                                                                    cAGAAATAAGGTCAACCCAAGTGGTGCTTTCGACTGCCCCATCCCCTGTGCTGCCATTACATCCGTAATTGTTAAACAACCGGTGCCTGTCGCTGGAGCGTCATCGCTCAATTTTGGCGATTTTGGGGAGTAATATTTTgg                                              >  1:123448/1‑141 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                              ACCACTTCGCGCAATACATTATAAAATCCCGTACCTGAACAATGCTCACAGCGAGCCTTACTTGCCGCAGACCTGGAGTAATCAGCAAAGGCAAAACTCACGAGGTAAGGAATAATCTGTAACCGGATTTCTTCACTCAATTTGTTCAATGTCGGGTTATCCAGTGCCATCGCGTAATTTAGCAGGCCTTCAATCGCAAACTGAGGGTCCTGAACACCAACTTTTGCCAGAAATAAGGCCAACCCAAGTGGTGCTTTCGACTGCACCATCCCCTGCGCTGCCATTACATCCGTAATTGTTAAACAACCGGTGCCTGTCGCTGGAGCGTCATCGCTCAATTTTGGAGATTTTGGGGAGTAATATTTTGGTAAGGCTTCAAGGTTCATGCTCGTTCTCCACTTACGCCAGTACG  >  NC_000913/1642856‑1643267

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑