Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,631,663	C→T	S208N (AGC→AAC)	ydfJ ←	putative transporter YdfJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,631,663	0	C	T	100.0%	27.4 / NA	11	S208N (AGC→AAC)	ydfJ	putative transporter YdfJ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (6/5);  total (6/5)

CTTTGTTAAACAATAACGTCTGCACTAAATAGCCTGCAAGGAAAGTCTGAATTAACCCGGAGTTACCCGCCTGACCAAAACGCAGCCCTGTTGCCAGCCAGAAGGATTTGCTCTGGAACATGCTACCAGCAGGTGCAGGTTTTGCTGTCGGTTGGTTACTGTCGTTAACCTTCTCAAAGACCGGGCTTTCTTTCAGATTCATACGCAACCAGATAGCAAAGACCATCACGACAACACTCGCCAGGAACGGTATACGCCATCCCCACGCCAGCAGTTCCTCTTTACTGAGAATGAAGAACATAAAGGCCCAGATTGCCGTTGCGCTCAAGGTTCCGCAGTTAGTTCCCAT  >  NC_000913/1631541‑1631889                                                                                                                           |                                                                                                                                                                                                                                   cTTTGNNCANNNATNNCGTCTGCACTAAATAGCCTGCAANGAAAGTCTGAATTAACCCGGAGTTACGCGNCNGNCCAANNCGCNGCCCTGTTGCNAGCCAGAAGGATTTGCTCTGGAACATGTTACCAGCAGGTGCAGGTTTTNCNGTCGGTTGGTTACTGTCGTTACCCTTCTCAANAACCGGGCTTTCTTTCAGATTCAtnagaanncanannnnnnnnn                                                                                                                                 >  2:161809/1‑202 (MQ=255)        aaaCAATAACGTCTGCACTAAATAGCCTGCAAGGAAAGTCTGAATTAACCCGGAGTGACCCGCCTGACCAAAACGCAGCCCAGTTGCCAGCCAGAAGGATTTGCTCTGGAACATGTTACCAGCAGGTGCAGGTTTTGCTGTCGGTTGGTTACTGTCGTTAACCTTCTCAAAGACCGGGCTTTCTGTCAGATTCATACGCAACCAGATAGCAAAGGCCATCACGGCAACGCTCGCCAGGAACGGTATACGc                                                                                              <  1:173486/250‑1 (MQ=255)                                      aaGGAAAGTCTGAATTAACCCGGAGTTACCCGCCTGACCAAAACGCAGCCCTGTTGCCAGCCAGAAGGATTTGCTCTGGAACATGTTACCAGCAGGTGCAGGTTTTGCTGTCGGTTGGTTACTGTCGTTAACCTTCTCAAAGACCGGGCTTTCTTTCAGATTCATACGCAACCAGATAGCAAAGACCATCACGACAACACTCGCCAGGAACGGTATACGCCATCCCCACGCCAGCAGTTCCTCTTTACTga                                                               <  1:8093/251‑1 (MQ=255)                                              tCTGAATTAACCCTGAGTTACCCGCCTGACCAAAACGCAGCCCTGTTGCCAGCCAGAAGGATTTGCTCTGGAACATGTTACCAGCAGGTGCAGGTGTTGCGGTCGGTTGGTTACTGTCGTTAACCTTCTCAAAGACCGGGctttctttn                                                                                                                                                             <  1:20233/149‑2 (MQ=255)                                                                  nccGCCTGACCAAAACGCAGCCCTGTTGCCAGCCAGAAGGATTTGCTCTGGAACATGTTACCAGCAGGTGCAGGTTTTGCTGTCGGTTGGTTACTGTCGTTAACCTTCTCAAAGACCGGGCTTTCTTTCAGATTCATACGCAACCAGATAGCAAAGACCATCACGACAACACTCGCCAGGAACGGTATACGCCATCCCCACGCCAGCAGTTCCTCTTTACTGAGAATGAAGAACATAAAGGCCCAGATTGc                                   >  1:20341/2‑251 (MQ=255)                                                                                     ncccTGTTGCCAGCCAGAAGGATTTGCTCTGGAACATGTTACCAGCAGGTGCAGGTTTTGCTGTCGGTTGGTTACTGTCGTTAACCTTCTCAAAGACCGGGCTTTCTTTCAGa                                                                                                                                                          >  1:132846/2‑113 (MQ=255)                                                                                     gCCCTGTTGCCAGCCAGAAGGATTTGCTCTGGAACATGTTGCCAGCAGGTGCAGGTTTTGCTGTCGGTTGGTTACTGTCGTTATCCTTCTCAAAGACCGGGCTTTCTTTCAGa                                                                                                                                                          <  2:132846/113‑1 (MQ=255)                                                                                       ccTGTTGCCAGCCAGAAGGATTTGCTCTGGAACATGTTACCAGCAGGTGCAGGTTTTGCTGTCGGTTGGTTACTGTCGTTAACCTTCTCAAAGACCGGGCTTTCTTTCAGATTCATACGCAACCAGATAGCAAAGACCATCACGACAACACTCGCCAGGAACGGTATACGCCATCCCCACGCCAGCAGTTCCTCTTTACTGAGAATGAAGAACATAAAGGCCCAGATTGCCGTTGCGCTCAAGGtt                   >  1:47832/1‑246 (MQ=255)                                                                                                 nccagAAGGATTTGCTCTGGAACATGTTACCAGCAGGTGCAGGTTTTGCTGTCGGTTGGTTACTGTCGTTAACCTTCTCAAAGACCGGGCTTTCTTTCAGa                                                                                                                                                          >  1:136147/2‑101 (MQ=255)                                                                                                 gccagAAGGATTTGCTCTGGAACATGTTACCAGCAGGTGCAGGTTTTGCTGTCGGTTGGTTACTGTCGTTAACCTTCTCAAAGACCGGGCTTTCTTTCAGa                                                                                                                                                          <  2:136147/101‑1 (MQ=255)                                                                                                   nagAAGGATTTGCTCTGGAACATGTTACCAGCAGGTGCAGGTTTTGCTGTCGGTTGGTTACTGTCGTTAACCTTCTCAAAGACCGGGCTTTCTTTCAGATTCATACGCAACCAGATAGCAAAGACCATCACGACAACACTCGCCAGGAACGGTATACGCCATCCCCACGCCAGCAGTTCCTCTTTACTGAGAATGAAGAACATAAAGGCCCAGATTGCCGTTGCGCTCAAGGTTCCGCAGTTAGTTCCCAt  >  1:23183/2‑251 (MQ=255)                                                                                                                           |                                                                                                                                                                                                                                   CTTTGTTAAACAATAACGTCTGCACTAAATAGCCTGCAAGGAAAGTCTGAATTAACCCGGAGTTACCCGCCTGACCAAAACGCAGCCCTGTTGCCAGCCAGAAGGATTTGCTCTGGAACATGCTACCAGCAGGTGCAGGTTTTGCTGTCGGTTGGTTACTGTCGTTAACCTTCTCAAAGACCGGGCTTTCTTTCAGATTCATACGCAACCAGATAGCAAAGACCATCACGACAACACTCGCCAGGAACGGTATACGCCATCCCCACGCCAGCAGTTCCTCTTTACTGAGAATGAAGAACATAAAGGCCCAGATTGCCGTTGCGCTCAAGGTTCCGCAGTTAGTTCCCAT  >  NC_000913/1631541‑1631889

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑