Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,586,597	C→T	intergenic (‑111/+223)	ydeP ← / ← ydeQ	putative oxidoreductase YdeP/putative fimbrial adhesin protein YdeQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,586,597	0	C	T	100.0%	13.2 / NA	6	intergenic (‑111/+223)	ydeP/ydeQ	putative oxidoreductase YdeP/putative fimbrial adhesin protein YdeQ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (4/2);  total (4/2)

CCTGGCGTATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCAACCACCTGCAGCACCCTGGTAGGATTCAATTTTTTTCTTCATCTACTTATCCTGTGTGGAAATGAATTATTAGGATTTGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTCTCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGCATCATCTCTCATAGTGTTTAAATTAACATATTTTTCAATATAAAAAATAAGACTCTGGCTTCAATTGTGCGCGGATTTTCTTACAGGTGTAGGCTAATAGCCTTCACAAAATAATTATCCGCGCAATTTTCGTGAATGGAGCGCATAGACTAAGGTCAAAAT  >  NC_000913/1586387‑1586814                                                                                                                                                                                                                   |                                                                                                                                                                                                                          ccTGGCGTATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCAAACACCTGCAGCACCCTGGTAGGATTCAATTTTTTTCTTCATCTACTTATCCTGTGTGGAAATGACTTATTAGGATTTGTAATAGCGAAGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATCTATAAGAGTAGGGGACTACTCTTTTTCTCTTTTTAATCTATTATCg                                                                                                                                                                                                    >  1:158188/1‑234 (MQ=255)                     ntNCGNNCTGCNTTCGCTACGGATTTAACAGCACCCNAACCACCTGCAGCACCCTGGTAGGATTCAATNTNTTTCTTCATCTACTTATCCTGTGTGGAAATGAATTATTAGGATTTGTANTAGCGATGATNCACNNTACANANANAGGGATTATCGCCAACATGGATATATAAGNGTAGGGGACTACTCTTTTTCTATNNTTNNNCTNNTATCg                                                                                                                                                                                                    <  2:158188/213‑1 (MQ=255)                                                   gCACCCCAACCACCTGCAGCACCCTGGTAGGATTCAATTTTTTTCTTCATCTACTTATCATGTGTGGAAATGAATTATTAGGATTTGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTCTTTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGCATCATCTCTCATCGTGTTTAAATTAACCTAttttt                                                                                                                                 >  1:154187/1‑251 (MQ=255)                                                                     gcaCCCTGGTAGGATTAAATTTTTTTCTTCATCTACTTATCCTGTGTGGAAATGACTTATTAGGATTTGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATACGAGTAGGGGACTACTCTTTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATACGTTAAg                                                                                                                                                                     >  1:178379/1‑197 (MQ=255)                                                                                                                                               gCGATGATGCACAGTACATACAAAGTGATTATCGCCAACATGGATATATAAGAGTAGTGGACTACTCTTTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGCATCATCTCTCATAGTGTTTAAATTTACATATTTTTCAATATAAAAAATAAGACTCTGGCTTc                                                                                                      <  1:99168/186‑1 (MQ=255)                                                                                                                                                                                  cAACATGGATATATAAGAGTAGGGGACTACTCTTTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGCATCATCTCTCATAGTGTTTAAATTAACATATTTTTCAATATAAAAAATAAGACTCTGGCTTCAATTGTGCGCGGATTTTCTTACAGGTGTAGGCTAATAGCCTTCACAAAATAATTATCCGCGCAATTTTCGTGAATGGAGCGCATAGACTAAGGTCAAAAt  >  1:110866/1‑251 (MQ=255)                                                                                                                                                                                                                   |                                                                                                                                                                                                                          CCTGGCGTATATCCATCTGCTTACGTACTGCATTCGCTACGGATTTAACAGCACCCCAACCACCTGCAGCACCCTGGTAGGATTCAATTTTTTTCTTCATCTACTTATCCTGTGTGGAAATGAATTATTAGGATTTGTAATAGCGATGATGCACAGTACATACAAAGGGATTATCGCCAACATGGATATATAAGAGTAGGGGACTACTCTCTTTCTATTTTTAATCTATTATCGTTAAACAATAGATTAAATACGATAAGTTAAGCATCATCTCTCATAGTGTTTAAATTAACATATTTTTCAATATAAAAAATAAGACTCTGGCTTCAATTGTGCGCGGATTTTCTTACAGGTGTAGGCTAATAGCCTTCACAAAATAATTATCCGCGCAATTTTCGTGAATGGAGCGCATAGACTAAGGTCAAAAT  >  NC_000913/1586387‑1586814

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑