Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,528,248	C→T	intergenic (+1096/‑1674)	yncH → / → ydcD	DUF5445 domain‑containing protein YncH/uncharacterized protein YdcD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,528,248	0	C	T	100.0%	23.6 / NA	11	intergenic (+1096/‑1674)	yncH/ydcD	DUF5445 domain‑containing protein YncH/uncharacterized protein YdcD
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (6/5);  total (6/5)

GACGGGGGAACTGCTGTGGCACCATGAGACCGGACATGCGTACAACGAACAGGGGCTGGCAAACCGCGTCACGCCGGACAGTCTGCCGCCGGTGGAGTGGCTGACCTACGGCAGCGGCTATCTTGCGGGCATGAAGCTGGGCGGGACGCCGCTGCTGGAGTTCACGCGCGACCGCCTGCACCGTGAGACGGTGCGCAGCTTCGGCAGCATGGCAGGCAGTAATGCCGCATATAAACTGACCAGCACATACACCCCCGCAGGCCAGTTACAGAGCCAGCACCTGAACAGCCTGGTGTATGACCGTGACTACGGGTGGAATGACAACGGCGACCTGGTGCGCATCAGCGGCCCGCGACAGACGCGGGAATACGGCTACAGCGCCACGGGCAGGCTGGAGAGTGTGCGC  >  NC_000913/1528009‑1528414                                                                                                                                                                                                                                                |                                                                                                                                                                       gaCGGGGGAACTGCTGTGGCACCATGAGACCGGACATGTGTACAACGAACAGGGGCTGGCAAACCGCGTCACGCCGGACAGTCTGCCGCCGGTGGAGTGGCTGACCTACGGCAGCGGCTATCTTGCGGGCATGAAGCTGGGCGGGACGCCGCTGCTGGAGTTCACGCGCGACCGCCTGCACCGTGAGACGGTGCGCAGCTTCGGCAGCATGGCAGGCAGTCATGCCGCATATAAACTGATCAGCACATac                                                                                                                                                              >  1:146140/1‑250 (MQ=255)                                                         tGGCAAACCGCGTCCCGCCGGACAGTCTGCCGCCGGTGGAGTGGCTGACGTACGGCAGCGGCTATCTTGCGGGCAGGAAGCTGGGCGGGACGCCGCTGTTGGAGTTCACGCGCGTCCGCCTGCACCGTGAGACGGTGCGCAGCTTCGGCAGCATGGCAGGCAGTAATGCCGCATATAAACTGATCAGCACATacac                                                                                                                                                            <  1:209890/196‑1 (MQ=18)                                                                    gTCACGCCGGACAGTCTGCCGCCGGTGGAGTGGCTGACCTACGGCAGCGGCCCTCTTGCGGGCATGAAGATGGGCGGGACGCCGCTGCTGGAGTTCACGCGCGCCCGCCTGCACCGTGAGACGGTGCGCAGCTTCGGCAGCATGGCAGGCAGTAATGCCGCATATAAACTGATCAGCACATACACCCCCGCAGGCCAGTTACAGAGCCAGCACCTGAACAGCCTGGTGTATGACCGTGACTACGGGTGGa                                                                                           >  1:146307/1‑250 (MQ=255)                                                                      cacgccagcccgTCTGCCGCCGGTGGAGTGGCTGACCTACGGCAGCGGCTATCTTGCGGGCATGAAGCTGGGCGGGACGCCGCTGCTGGAGTTCACGCGCGACCGCCTGCACCGTGAGACGGTGCGCAGCTTCGGCAGCATGGCAGGCAGTAATGCCGCATATAACCTGATCAGCACATACACCCCCGCAGGCCAGTTACAGAGCCAGCACCTGAACAGCCTGGTGTATGACCGTGACTACGGGTGGAATg                                                                                        >  1:51661/12‑251 (MQ=255)                                                                             gACAGGCTGCCGCCGGTGGAGTGGCTGACCTACGGCAGCGGCTATCTTGCGGGCATGAAGCTGGGCGGGACGCCGCTGCTGGAGTTCACGCGCGACCGCCTGCACCGTGAGACGGTGCGCAGCTTCGGCAGCATGGCAGGCAGTAATGCCGCATATAAACTGATCAGCACATACACCCCCGCAGGCCAGTTACAGAGCCAGCACCTGAACAGCCTGGTGTATGACCGTGACTACTGGTGGAATGACAACgg                                                                                 <  1:168750/251‑1 (MQ=255)                                                                                                     cTGACCTACGGCAGCGGCTATCTTGCGGGCATGAAGCTGGGCGGGACGCCGCTGCTGGAGTTCACGCGCGACCGCCTGCACCGTGAGACGGTGCGCAGCTTCGGCAGCATGGCAGGCAGTAATGCCGCATATAAACTGATCAGCACATACACCCCCGCAGGCCAGTTACAGAGCCAGCACCTGAACAGCCTGGTGTATGACCGTGACTACGGGTGGAATGACAAc                                                                                   >  1:176873/1‑225 (MQ=255)                                                                                                                                                             ggAGTTCACGCGCGCCCGCCTGCACCGGGAGACGGTGCGCAGCTTCGGCAGCATGGCAGGCAGTAAGGCCGCATATAAACTGATCAGCTCATACACCCCCGCATGCCAGTTACAGAGCCAGCTCCTGAACAGCCGGGTTTATGGCCGTGACTTCTGGTGGAAGGACAACGGCGACCTGGTTCGCATCAGCGGCCCGCGACAGACGCGGGAATTCGGCTACAGCGCCACGGGCAGGCGGGAGAGTGTgcgc  <  1:164235/250‑1 (MQ=14)                                                                                                                                                                            nngCCTGCNNCGTGAGACGGTGCGCAGCTTCGGCAGCATGGCAGGCAGTAATGCCGCATATGAACTGATCAGCACATACACCCCCGCAGGCCAGTTAc                                                                                                                                           <  2:6084/96‑1 (MQ=11)                                                                                                                                                                            ccGCCTGCACCGTGAGACGGTGCGCAGCTTCGGCAGCATGGCAGGCAGTAATGCCGCATATGAACTGATCAGCACATACACCCCCGCAGGCCAGTTAc                                                                                                                                           >  1:6084/1‑98 (MQ=255)                                                                                                                                                                               ncTGCACCGTGAGACGGTGCGCAGCTTCGGCAGCATGGCAGGCAGTAATGCCGCATATAAACTGATCAGCACATac                                                                                                                                                              >  1:131201/2‑76 (MQ=255)                                                                                                                                                                               ccTGCACCGTGAGACGGTGCGCAGCTTCGGCAGCATGGCAGGCAGTAATGCCGCATATAAACTGATCAGCACATac                                                                                                                                                              <  2:131201/76‑1 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                       GACGGGGGAACTGCTGTGGCACCATGAGACCGGACATGCGTACAACGAACAGGGGCTGGCAAACCGCGTCACGCCGGACAGTCTGCCGCCGGTGGAGTGGCTGACCTACGGCAGCGGCTATCTTGCGGGCATGAAGCTGGGCGGGACGCCGCTGCTGGAGTTCACGCGCGACCGCCTGCACCGTGAGACGGTGCGCAGCTTCGGCAGCATGGCAGGCAGTAATGCCGCATATAAACTGACCAGCACATACACCCCCGCAGGCCAGTTACAGAGCCAGCACCTGAACAGCCTGGTGTATGACCGTGACTACGGGTGGAATGACAACGGCGACCTGGTGCGCATCAGCGGCCCGCGACAGACGCGGGAATACGGCTACAGCGCCACGGGCAGGCTGGAGAGTGTGCGC  >  NC_000913/1528009‑1528414

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑