Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,512,277	C→T	S208S (AGC→AGT)	ydcS →	putative ABC transporter periplasmic binding protein/polyhydroxybutyrate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,512,277	0	C	T	100.0%	23.0 / NA	10	S208S (AGC→AGT)	ydcS	putative ABC transporter periplasmic binding protein/polyhydroxybutyrate synthase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (7/3);  total (7/3)

GGTTTAATGTTGGCGGCAAAGTTTACGGCACACCTTACCAATGGGGGCCGAACCTGCTGATGTACAACACTAAAACCTTCCCGACGCCGCCGGATAGCTGGCAAGTGGTTTTTGTTGAGCAAAATCTGCCGGACGGCAAGAGCAATAAAGGCCGCGTTCAGGCTTATGATGGCCCTATCTATATTGCGGACGCTGCGTTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGTGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCAAAAACGAGGGTGTGGTTGCTTCCAGTGCCTGGCCCTATCAGGCCAACGCCCTGAAAGCCGAAGGCCAGCCTGTTGCTACCGTTTTCCCGA  >  NC_000913/1512042‑1512482                                                                                                                                                                                                                                            |                                                                                                                                                                                                              ggTTTAATGTTGGCGGCAAAGTTTACGGCACACCTTACCAATGGGGGCCGAACCTGCTGATGTACAACACTAAAACCTTCCCGACGCCGCCGGATAGCTGGCGAGTGGTTTTTGTTGAGCAAAATCTGCCGGACGGCAAGAGCAATAAAGGCCGCGTTCAGGCTTATGATGGCCCTATCTATATTGCGGACGCTGCGTTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGTGATCCGTATCAACTc                                                                                                                                                                                                <  1:213670/251‑1 (MQ=255)                                       cAATGGGGGCCGAACCTGCTGATGTACAACACTAAAACCTTCCCGACGCCGCCGGATAGCTGGCAAGTGGTTTTTGTTGCGCAACATCTGCCGGACGGCAAGAGCAATAAAGGCCGCGTTCAGGCTTATGATGGCACTATCTATATTGTGGACGCTGCGTTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGTGCTCCGTATCAACTCACCGAAGAACAGTACCAGGCGGTGCTGAAAGTGCTgcg                                                                                                                                                          >  1:220909/1‑251 (MQ=255)                                       cAATGGGGGCCGAACCTGCTGATGTACAACACTAAAACCTTCCCGACGCCGCCGGATAGCTGGCAAGTGGCTTTTGTTGAGCAAAATCTGCCGGACGGCAAGAGCAATAAAGGCCGCGTTCAGGCTTATGATGGCCCTATCTATATTGCGGACGCTGCGTTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGTGATCCGTATCAACTCACCGAAGAAc                                                                                                                                                                                      >  1:197178/1‑223 (MQ=255)                                                                                                                                 ncGGACGGCAAGAGCAATAAAGGCCGCGTTCAGGCTTATGATGGCCCTATCTATATTGCGGACGCTGCGTTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGTGATCCGTATCAACTCACCGAAGAACAGTACCAGGc                                                                                                                                                                            >  1:19209/2‑143 (MQ=255)                                                                                                                                                aaTAAAGGCCGCGTTCAGGCTTATGATGGCCCTATCTATATTGCGGACGCTGCGTTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGTGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGTGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCAAAAACGAGGGTGTGGTTGCTTCCAGTGCCTGGCCCTATCAGGc                                                 <  1:91863/251‑1 (MQ=255)                                                                                                                                                            nttCAGGCTTATGATGGCCCTATCTATATTGCGGACGCTGCGTTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGTGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGTGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCAAAAACGAGGGTGTGGTTGCTTCCAGTGCCTGGCCCTATCAAGCCAACGCCCTGa                                      >  1:22646/2‑250 (MQ=255)                                                                                                                                                                            gCCCTATCTATATTGCGGACGCTGCGTTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGTGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGTGCTGAAAGTGCTGCGCGCTCAACACAGTTTTATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCAAAAACGAGGGTGTGGTTGCTTCCAGTGCCTg                                                             <  1:194156/211‑1 (MQ=255)                                                                                                                                                                                                 ntGCGTTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGTGATCCGTATCAACTCCCCGAAGAACAGTACCAGGCGGTGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCAAAAACGAGGGTGTGGTTGCTTCCAGTGCCTGGCCCTATCAGGCCAACGCCCTGAAAGCCGAAGACCAGCCTGTTGCTACCGTTTTCCCGa  >  1:40744/2‑249 (MQ=255)                                                                                                                                                                                                               aaaGCCACTCAGCCGCAGTTGGGCATCAGTGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGTGCTGAAAGTGATGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCAAAAACGAGGGTGTGGTTGCTTCCAGTGCCTGGCCCTATCAGGCCAACGCGCTGAAAGCCGAAGGCCAGCCTGTTGCTACCGTTTTc      >  1:29208/1‑231 (MQ=255)                                                                                                                                                                                                                                    ggCATCAGTGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGTGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCAAAAACGAGGNTGTNNNNNCt                                                                        >  2:130468/1‑144 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                              GGTTTAATGTTGGCGGCAAAGTTTACGGCACACCTTACCAATGGGGGCCGAACCTGCTGATGTACAACACTAAAACCTTCCCGACGCCGCCGGATAGCTGGCAAGTGGTTTTTGTTGAGCAAAATCTGCCGGACGGCAAGAGCAATAAAGGCCGCGTTCAGGCTTATGATGGCCCTATCTATATTGCGGACGCTGCGTTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGTGCTGAAAGTGCTGCGCGCTCAACACAGTTTGATCCATCGCTACTGGCATGACACTACCGTGCAAATGAGCGATTTCAAAAACGAGGGTGTGGTTGCTTCCAGTGCCTGGCCCTATCAGGCCAACGCCCTGAAAGCCGAAGGCCAGCCTGTTGCTACCGTTTTCCCGA  >  NC_000913/1512042‑1512482

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑