Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,489,624	G→A	G465S (GGC→AGC)	aldA →	aldehyde dehydrogenase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,489,624	0	G	A	100.0%	33.8 / NA	13	G465S (GGC→AGC)	aldA	aldehyde dehydrogenase A
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (7/6);  total (7/6)

GAAACCTTTGGCCCGGTGCTGCCAGTTGTCGCATTTGACACGCTGGAAGATGCTATCTCAATGGCTAATGACAGTGATTACGGCCTGACCTCATCAATCTATACCCAAAATCTGAACGTCGCGATGAAAGCCATTAAAGGGCTGAAGTTTGGTGAAACTTACATCAACCGTGAAAACTTCGAAGCTATGCAAGGCTTCCACGCCGGATGGCGTAAATCCGGTATTGGCGGCGCAGATGGTAAACATGGCTTGCATGAATATCTGCAGACCCAGGTGGTTTATTTACAGTCTTAATGAGTGAAAGAGGCGGAGGTTTTTTCCTCCGCCTGTGCGCGTCAGAGTTTAGCGAATTTTTCGAGGGTGCGAATAAGCTGTGTGACGAAGCCATATTCGTTATCGTACCAGGCGACCGTTTTCACCAGTTGTAAATCGCCCACGGCGGTAATTTCCGTTTGCGTGGCATCAAACACCGAAC  >  NC_000913/1489378‑1489852                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                     gAAACCTTTGGCCCGGTGCTGCCAGTTGTCGCATTTGACACGCTGGAAGATGCTATCTCAATGGCTAATGACAGTGATTACGGCCTGACCTCATCAATCTATACCCAAAATCTGAACGTCGCGATGACAGCCATTAAAGGGCTGACGTTTGGTGAAACTTACATCAACCGTGAAAACTTCGAAGCTATGCAAGGCTTCCACGCCGGATGGCGTAAATCCGGTATTGGCGGCGCAGATGGTAAACATAGCtt                                                                                                                                                                                                                                  >  1:81140/1‑251 (MQ=255)                              cGCATTTGACACGCTGGAAGATGCTATCTCAATGGCTACTGACAGTGATTACGGCCTGCCCTCATCAATCTATACCCAACATCTGAACGTCGCGATGAACGCCATTAAAGGGCTGAAGTTTGGTGAAACTTACATCAACCGTGAAAACTTCGAAGCTATGCAAGGCTTCCACGCCGGCTGGCGTAAATCCGGTATTGGCGGCGCAGATGGTAAACATAGCTTGCATGAATATCTGCAGACCCAGGTGGttt                                                                                                                                                                                                     >  1:86284/1‑251 (MQ=255)                                                                                                          cAAAATCTGAACGTCGCGATGAAAGCCATTAAAGGGCTGAAGTTTGGTGAAACTTACATCAACCGTGAAAACTTCGAAGCTATGCAAGGCTTCCACGACGGATGGCGTAAATCCGGTCTTGGCGGCGCAGATGGTAAACATAGCTTGCTTGAATATCTGCAGACCCAGGTGGTTTATTTACAGTCTTAATGAGTGAAAGAGGCGGAGGTTTTTTCCTCCGCCTGTGCGCGTCAGAGTTTAGCGAATTTTTc                                                                                                                         >  1:217062/1‑251 (MQ=255)                                                                                                                                                       ngTGAAACTTACATCAACCGTGAAAACTTCGAAGCTATGCACGGCTTCCACGCCGGATGGCGTAAATCCGGTATTGGCGGCGCAGATGGTAAACATAGCTTGCATGAATATCTGCa                                                                                                                                                                                                                   >  1:40929/2‑116 (MQ=255)                                                                                                                                                                          gTGAAACCTTCTAAGCTATGCAAGGCTGCCCCGCCTGATGTCAGAAATCCGGTAGGGGCGGCGCAGCTGGTCAACATATCTTGCATGAATAGCTGCAGGCTCAGGTGGTTTATTTACAGTCTTAATGCGTGAAATAGGCGGAGGTTTTTTCCTCCGCTTGTGCGCGTCAGAGGTTAGCGAATTTTTc                                                                                                                         <  1:38031/187‑1 (MQ=255)                                                                                                                                                                                 cTTCGAAGCTATGCAAGGCTTCCACGCCGGATGGCGTAAATCCGGTATTGGCGGCGCAGATGGTAAACATAGCTTGAATGAATATCTGCAGACCCAGGTGGTTTATTTACAGTCTTAATGAGTGAAAGAGGCGGAGGTTTTTTCCTCCGCCTGTGCGCGTCAGAGTTTAGCGAATTTTTCGAGGGTGCGAATAAGCTGTGTGACGAAGCCATATTCGTTATCGTACCAGGCGCCAGTTTTCACCAGTTGt                                                   >  1:149370/1‑250 (MQ=255)                                                                                                                                                                                       aGCTATGCAAGGCTTCCACGCCGGATGGCGTAAATCCGGTATTGGCGGCGCAGATGGTAAACATAGCTTGCATGAATATCTGCAGACCCAGGTGGTTTATTTACAGTCTTAATGAGTGAAAGAGGCGGAGGTTTTTTCCGCCGCCTGTGCGCGTCAGAGTTGAGCGAATTTTTCGAGGGTGCGAATAAGCTGTGTGACGAAGCCATATTCGTTATCGTACCAggn                                                                      <  1:44902/225‑2 (MQ=255)                                                                                                                                                                                                aggattCCACGCCGGAGGGCGTAAATCCGGTATTGGCGGCGCAGATGGTAAACATAGCTTGCATGAATATCTGCAGACCCAGGTGGTTTATTTACAGTCTTAATGAGTGAAAGAGGCGGAGGTTTTTTCCGCCGCCTGTGCGCGTCAGAGTTTAGCGAATTTTTCGAGGGTGCGAATAAGCTGTGTGACGAAGCCATATTCGTTATCGTACCAGGCGACCGTTTTCACCAGTTGTAAATCGCCCAcggcgn                                   <  1:55443/247‑2 (MQ=255)                                                                                                                                                                                                 ggCTTCCACGCCGGATGGCTTAAATCCGGTATTGTCGGCGCAGATGGTAAACATAGCTTGCATGAATATCTGCAGACCCATGTGGTTGATTTGCAGTCTTTAGGAGTGAAAGAGGCGGAGGTTTTTTCCTCCGCCTGTGCGCGTCAGAGTTTAg                                                                                                                                   <  1:192022/154‑1 (MQ=255)                                                                                                                                                                                                                  gCGTANATCTGNTNNTGGCGGCGCAGATGGGAAANATAGCTTGCATGAATNTCTGNAGAccn                                                                                                                                                                                                              >  2:86415/1‑61 (MQ=255)                                                                                                                                                                                                                      aaaTCCGGTATTGGCGGCGCAGATGGTAAACATAGCTTGCATGAATATCTGCAGACCCAGGTGGTTTATTTACAGTCTTAATGAGTGAAAGAGGCGGAGGTTTTTTCCTCCGCCTGTGCGCGTCAGAGTTTAGCGAATTTTTCGAGGGTGCGAATAAGCTGTGTGACGAAGCCATATTCGTTATCGTACCAGGCGACCGTTGTCACCAGTTGTAAATCGCCCACTGCGGTAAGTTCCGTTTGCGTGGCAt              <  1:69461/250‑1 (MQ=255)                                                                                                                                                                                                                                        gcAGATGGTAAACATAGCTTGCATGAATATCTGCAGACCCAGGTGTTTTATTTACAGTCTTAATGAGTGAAAGAGGCGGAGGTTTTTTCCTCCGCCTGTGCGCTTCAGAGTTTAGCGAATTGTTCGAGGGTGCGAATAAGCTGTGTGACGAAGCCATATTCGTTATCGTACCAGGCGACCGTTTTCAc                                                          <  1:200340/188‑1 (MQ=255)                                                                                                                                                                                                                                               gTAAACATAGCTTGCATGAATATCTGCAGACCCAGGTGGTTTATTTACTGTCTTAATGAGTGAAAGAGGCGGAGGTTTTTTCCTCCGCCTGTGCGCGTCAGAGTTTAGCGAATTTTTCGAGGGTGCGAATAAGCTGTGTGACGAAGCCATATTCGTTATCGTACCAGGCGACCGTTTTCACAAGTTGTAAATCGCCCACGGCGGTAATTTCCGTTTGCGGGGCCTAAAAAaccgaac  >  1:208865/1‑237 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                     GAAACCTTTGGCCCGGTGCTGCCAGTTGTCGCATTTGACACGCTGGAAGATGCTATCTCAATGGCTAATGACAGTGATTACGGCCTGACCTCATCAATCTATACCCAAAATCTGAACGTCGCGATGAAAGCCATTAAAGGGCTGAAGTTTGGTGAAACTTACATCAACCGTGAAAACTTCGAAGCTATGCAAGGCTTCCACGCCGGATGGCGTAAATCCGGTATTGGCGGCGCAGATGGTAAACATGGCTTGCATGAATATCTGCAGACCCAGGTGGTTTATTTACAGTCTTAATGAGTGAAAGAGGCGGAGGTTTTTTCCTCCGCCTGTGCGCGTCAGAGTTTAGCGAATTTTTCGAGGGTGCGAATAAGCTGTGTGACGAAGCCATATTCGTTATCGTACCAGGCGACCGTTTTCACCAGTTGTAAATCGCCCACGGCGGTAATTTCCGTTTGCGTGGCATCAAACACCGAAC  >  NC_000913/1489378‑1489852

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑