Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,401,843	G→A	I471I (ATC→ATT)	abgB ←	p‑aminobenzoyl‑glutamate hydrolase subunit B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,401,843	0	G	A	100.0%	20.6 / NA	10	I471I (ATC→ATT)	abgB	p‑aminobenzoyl‑glutamate hydrolase subunit B
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (4/6);  total (4/6)

CGGTCGGGTTTTTCGCACTGACGCCAAAGGCCGACAAAATTGCCGTCGTCACCATGAGTACGATAATCAAATAGATAAAGAGCAGAAAAGGATGGGGAACCTTGTTACCAATTCTTTCGACCCAGCCATAGAGCTTCCCGGATTGGGAGGACGACGGTATGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGATAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGACTGTGGTTGCTGCCATAGTTTTCGCCGCCAGCAGCATTCCTTTATGAGCAATAGATGTTCGCCCCTGGCTAACCAGTTGCCACGTATGTAGCGGTGTACCGACG  >  NC_000913/1401599‑1402035                                                                                                                                                                                                                                                     |                                                                                                                                                                                                 cggtcggGTTTTTCGCACTGACGCCAAAGGCCGACAAAATTGCCGTCGTCACCATGAGTACGATAATCAAATAGATAAAGAGCAGAAAAGGATGGGGAACCTTGTTACCAATTCTTTCGACCCAGCCATAGAGCTTTCCGGATTGGGAGGACGACTGTATGGATGACATCCTCATGGGCATTCCTCGGGTTTTGTGTTTGCGTTGTTGGTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGAATAggg                                                                                                                                                                                            <  1:31930/251‑1 (MQ=255)       ggTTTTTCGCACTGCCGCCAAAGGCCGACAAAATTGCCGTCGTCACCATGAGTACGATAATCAAATAGATAAAGAGCAGAAAAGGATGGGGAACCTTGTTACCAATTCTTTCGACCCAGCCATAGAGCTTCCCGGATTGGGAGGACGACGGTATGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGAATAGGGCAGTg                                                                                                                                                                                       >  1:122654/1‑250 (MQ=255)                                                        gAGTACGATAATCAAATAGATAAAGAGCAGAAAAGGATGGGGAACCTTGTTACCAATTCTTTCGACCCAGCCATAGAGCTTCCCGGATTGGGAGGACGACGGTATGGATGACATACTCATGGGCATTCCGCGGTTGTTGTGGTTGCGTTGTTGTTGTTATGTTAAAGGTGACGGTGTCTCGTTTTTCGGAATAGGGCAGTTATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGc                                                                                                                                      <  1:117819/250‑1 (MQ=255)                                                                                              ggggAACCTTGTTACCAATTCTTTCGACCCAGCCATAGAGCTTCCCGGATTGGGAGGACGACGGTATGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGAATAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGACTGTGGTtgc                                                                                                <  1:101846/250‑1 (MQ=255)                                                                                                                              ccATAGAGCTTCCCGGATTGGGAGGACGACGGTATGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGAATAGGGCAGTGATACGGTTGCGTGTCCGTTACTTg                                                                                                                                                               >  1:37546/1‑155 (MQ=255)                                                                                                                              ccATAGAGCTTCCCGGATTGGGAGGACGACGGTATGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGAATAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGACTGTGGTTGCTGCCATAGTTTTCGCCGCcagc                                                                          >  1:113125/1‑240 (MQ=255)                                                                                                                                          ncGGATTGGGAGGACGACGGTATGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGAATAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGACTGTGGTTGCTGCCATAGTTTTCGCCGCCAGCAGCAt                                                                     >  1:132515/2‑233 (MQ=255)                                                                                                                                                                                       ccTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGAATAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGACTGTGGTTGCTGCCATAGTTTTCGCCGCCAGCAGCATTCCTTTATGAGCAATAGATGTTCGCCCCTGGCTAACCAGTTGCCACGTATGTAGCGGTGTAc       <  1:11585/250‑1 (MQ=255)                                                                                                                                                                                           ggTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGAATAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGACTGTGGTTGCTGCCATAGTTTTCGCCGCCAGCAGCATTCCTTTATGAGCAATAGATGTTCGCCCCTGGCTAACCAGTTGCCACGTATGTAGCGGTGTACCGACg  <  1:162344/251‑1 (MQ=255)                                                                                                                                                                                                                                         naCGNTTTTCGGAATAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGACTGTGGTTGCTGCCATAGTTTTCGCCGCCAGCAGCAt                                                                     <  2:132515/137‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                 CGGTCGGGTTTTTCGCACTGACGCCAAAGGCCGACAAAATTGCCGTCGTCACCATGAGTACGATAATCAAATAGATAAAGAGCAGAAAAGGATGGGGAACCTTGTTACCAATTCTTTCGACCCAGCCATAGAGCTTCCCGGATTGGGAGGACGACGGTATGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGATAGGGCAGTGATACGGTTGCGTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGACTGTGGTTGCTGCCATAGTTTTCGCCGCCAGCAGCATTCCTTTATGAGCAATAGATGTTCGCCCCTGGCTAACCAGTTGCCACGTATGTAGCGGTGTACCGACG  >  NC_000913/1401599‑1402035

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑