Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,107,648	G→A	G432R (GGA→AGA)	clsC →	cardiolipin synthase C

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,107,648	0	G	A	100.0%	30.9 / NA	14	G432R (GGA→AGA)	clsC	cardiolipin synthase C
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (9/5);  total (9/5)

ACAAAGTAGTACGTTACACGATCGCGGCATAACCGGTAATTCCGGAGCCAGCCTGCATGCTAAAACCTTTAGCATCGATGGTAAAACGGTGTTTATCGGCTCTTTCAATTTCGATCCGCGTTCAACATTGCTCAATACTGAAATGGGCTTCGTGATAGAGAGCGAAACGCTGGCACAGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTATTGTAAATGTGGCTTAACGGGCTGGTGCTTTATCGTTCTCACGCTTAACAACC  >  NC_000913/1107404‑1107823                                                                                                                                                                                                                                                     |                                                                                                                                                                                ncAAAGTAGTACGTTACACGATCGCGGCATAACCGGTAATTCCGGAGCCAGCCTGCATGCTAAAACCTTTAGCATCGATGGTAAAACGGTGTTTATCGGCTCTTTCAATTTCGATCCGCGTTCAACATTGCTCAATACTGAAATGGGCTTCGTGATAGAGAGCGAAACGCTGGCACAGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGAGACgg                                                                                                                                                                            >  1:175353/2‑250 (MQ=255)                acacGATCGCGGCATAACCGGTAATTCCGGAGCCAGCCTGCATGCTAAAACCTTTAGCATCGATGGTAAAACGGTGTTTATCGGCTCTTTCAATTTCGATCCGCGTTCAACATTGCTCAATACTGAAATGGGCTTCGTGATAGAGAGCGAAACGCTGGCACAGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGAGACGGATCAACTTGGTTGAtn                                                                                                                                                            <  1:54724/251‑2 (MQ=255)                                    gTAATTCCGGAGCAAGCCTGCATGCTAAAACCTTTAGCATCGATGGTAAAACGGTGTTTATCGGCTCTTTCAATTTCGATCCGCGTTCAACATTGCTCAATACTGAAATGGGCTTCGTGATAGAGAGCGAAACGCTGGCACAGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGAGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGatt                                                                                                                                        >  1:172894/1‑251 (MQ=255)                                               nccagccTGCATGCTAAAACCTTTAGCATCGATGGTAAAACGGTGTTTATCGGCTCTTTCAATTTCGATCCGCGTTCAACATTGCTCAATACTGAAATGGGCTTCGTGATAGAGAGCGAAACGCTGGCACAGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGAGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCaaaa                                                                                                                              >  1:134096/2‑250 (MQ=255)                                                                                ggTAAAACGGTGTTTATCGGCTCTTTCAATTTCGATCCGCGTTCAACATTGCTCAATACTGAAATGGGCTTCGTGATAGAGAGCGAAACGCTGGCACAGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGAGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTa                                                                                             <  1:89995/250‑1 (MQ=255)                                                                                                         tCAATTTCGATCCGCGTTCAACATTGCTCAATACTGAAATGGGCTTCGTGATAGAGAGCGAAACGCTGGCACAGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATTCGGCCTGGCAGCTCCGTCTGGACAGGTGGAGGCGGATCAACTGTGTTGATCGTTAGGCAAAGAAAGAGAGTATGCTCGAAAAATATCCCTCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGcc                                                                    <  1:163308/250‑1 (MQ=255)                                                                                                                         ttCAACATTGCTCAATACTGAAATGGGCTTCGTGATAGAGAGCGAAACGCTGGCACAGTTAATTGATAAACGCTTTATTCAGAGCCAGAATGATGCGGCCTGGCAGCTCCGTCTGAACAGGTGGAGACGGATCAACTGGGTTGATCGTTATGCAAAGAAAGAGATTATTCTCACAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTATTGt                                                   >  1:220658/1‑251 (MQ=255)                                                                                                                                     cAATACTGAAATGCGCTTCGTGATAGAGAGCGAAACGCTGGCACAGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGAGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTATTGTAAATGTGGCtt                                        >  1:68259/1‑250 (MQ=255)                                                                                                                                          cTGAAATGGGCTTCGTGATAGAGAGCGAAACGCTGGCACAGTTAATTGATAAACGCTTTATTCAGAGCAAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGAGACAGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTATTGTAAATGTGGCTTAACggg                                  >  1:126259/1‑251 (MQ=255)                                                                                                                                                   gCTTCGTGATAGAGAGCGAAACGCTGGCACAGTTAATTGATAAACGCTTTATTCCGAGCCCGTATGATGCGGACTGGCAGCTCCGTCTGGCCAGGTGGAGACGGATCTACTGGGTTGATCGTCTTGCAAAGAAAGAGATTATTCTCa                                                                                                                                 >  1:29606/1‑147 (MQ=255)                                                                                                                                                                           tGGCACAGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGAAGCTACGTCTGGACAGGTGGAGACGGATAAACTGGGTTGATCGTCATCCAAAGAAAGAGATTATTCTCAAAAAAg                                                                                                                           >  1:195219/1‑129 (MQ=255)                                                                                                                                                                                                         agagCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGAGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGcc                                                                    <  1:34043/154‑1 (MQ=255)                                                                                                                                                                                                                          nnnnnncANNNNNGTCNGGACAGGTGGAGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTa                                                       <  2:134096/144‑1 (MQ=255)                                                                                                                                                                                                                              ggCAGCTCCGTCTGGACAGGTGGAGACGGATCAACTGGGATGATCGTCATGCAAAGACAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTATTGTAAATGTGGCTTAACGGGCTGGTGCTTTATCGTTTTCACGCTTAACAAcc  >  1:146430/1‑199 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                ACAAAGTAGTACGTTACACGATCGCGGCATAACCGGTAATTCCGGAGCCAGCCTGCATGCTAAAACCTTTAGCATCGATGGTAAAACGGTGTTTATCGGCTCTTTCAATTTCGATCCGCGTTCAACATTGCTCAATACTGAAATGGGCTTCGTGATAGAGAGCGAAACGCTGGCACAGTTAATTGATAAACGCTTTATTCAGAGCCAGTATGATGCGGCCTGGCAGCTCCGTCTGGACAGGTGGGGACGGATCAACTGGGTTGATCGTCATGCAAAGAAAGAGATTATTCTCAAAAAAGAACCCGCCACCAGTTTCTGGAAGCGGGTTATGGTCAGACTGGCGTCGATATTGCCCGTGGAATGGTTATTGTAAATGTGGCTTAACGGGCTGGTGCTTTATCGTTCTCACGCTTAACAACC  >  NC_000913/1107404‑1107823

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑