Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	251,499	G→A	G201D (GGT→GAT)	dinB →	DNA polymerase IV

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	251,499	0	G	A	93.8%	40.1 / NA	16	G201D (GGT→GAT)	dinB	DNA polymerase IV
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (6/9);  minor base C (1/0);  total (7/9)
Fisher's exact test for biased strand distribution p-value = 4.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGAAATCCGCCAGACAATCTTCAACGAGCTGCAACTGACGGCGTCTGCGGGCGTGGCACCAGTAAAGTTTCTCGCCAAAATCGCCTCCGACATGAATAAACCCAACGGCCAGTTTGTGATTACGCCGGCAGAAGTTCCGGCATTTTTACAAACCTTACCGCTGGCAAAAATCCCCGGCGTCGGCAAAGTCTCAGCGGCAAAACTGGAAGCGATGGGGCTGCGGACCTGCGGTGATGTACAAAAGTGTGATCTGGTGATGCTGCTTAAACGCTTTGGCAAATTTGGCCGCATTTTGTGGGAGCGTAGTCAGGGGATTGACGAACGCGATGTTAACAGCGAACGGTTGCGAAAATCCGTCGGCGTGGAACGCACGATGGCGGAAGATATTCA  >  NC_000913/251269‑251658                                                                                                                                                                                                                                       |                                                                                                                                                                ggAAATCCGCCAGAAAATCTTCAACGAGTTGCAACTGACGGCGTCTGCGGGCGGGGCAACAGTAAAGTTTCTCGCCAAAATCGCCTCCGACATGAATAAACCCAACGGCCAGTTTGTGATTACGCCGGCAGAAGTTCCGGCATTTTTATGAACCTTACCGCTGGCAAAAATCCCCGGCGTCGACAAAGTCTCAGCGGCAAAACTGGAAGCGATGGGGCTGCGGACCTGCGATGATGTACACAAGTGTGATc                                                                                                                                             <  1:155416/251‑1 (MQ=255)  gAAATCCGCCAGACAATCTTCAACGACCTGCAACTGATGGCGTCTGCGGGCGTGGCCCAAGTAAAGATTCTCGCCAAAATCGCCTCCGACATGAATAAACCCAATGGCCAGTTTGTGATTACGCCGGCAGAAGTTCCGGCATTTTTACAAACCTTACCGCTGGCAAAAATCCCCGGCGTCGGCAAAGTCTCAGCGGCAAAACTGGAAGCGATGGGGCTGCGGACCTGCGATGATGTACAAAAGTGTGATCt                                                                                                                                            >  1:202158/1‑251 (MQ=255)          ccAGACAATCTTCAACGAGCTGCAACTGACGGCGTCTGCGGGCGTGGCACCAGTAAAGTTTCTCGCCAAAATCGCCTCCGACATGAATAAACACAACGGCCAGTTTGTGATTACGCCGGCAGAAGTTCCGGCATTTTTACAAACCTTACCGCTGGCAAAAATCCCCGGCGTCGGCAAAGTCTCAGCGGCAAAACTGGAAGCGATGGGGCTGCGGACCTGCGATGATGTACAAAAGTGTGATCTGGTGAtgc                                                                                                                                    >  1:193569/1‑251 (MQ=255)                         cGAGCTGCAACTGACGGCGTCTGCGGGCGTGGCACCAGTAAAGTTTCTCGCCAAAATCGCCTCCGACAGGAATAAACCCAACGGCCAGTTTGTGATTACGCCGGCAGAAGTTCCGGCATTTTTACAAACCTTACCGCTGGCAAAAATCCCCGGCGTCGGCAAAGTCTCAGCGGCAAAACTGGAAGCGATGGGGCTGCGGACCTGCGATGAGGTACAAAAGTGTGATCTGGTGATGCTGCTTAAACGCTTTg                                                                                                                     <  1:100264/251‑1 (MQ=255)                                           gTCTGCGGGCGTGGCACCAGTAAAGTTTCTCGCCAAAATCGCCTCCGACATGAATAAACCCAACGGCCAGTTTGTGATTACGCCGGCAGAAGTGCCGGCATTTTTACAAACCTTACCGCTGGCAAAAATCCCCGGCGTCGGCAAAGTCTCAGCGGCAAAACTTGAAGCGATGGGGCTGCGGACCTGCGATGATGTACAAAAGTGTGATCTGGTGATGCTGCTTAAACGCTTTGGCAAATTTGGCCGCAttt                                                                                                   <  1:200843/251‑1 (MQ=255)                                                        gCACCAGTAAAGTTTCTCGCCAAAATCGCCTCCGACATGAATAAACCCAACGGCCAGTTTGTTATTACGCCGGCATAAGTTCCGGCATTTTGACAAACCTTACCGCTGGCAAAAATCCCCGGCTTCGGCAAAGTCGCAGCGGCAAAACTTGAAGCTATGTGGCTGCGTACCTGCGATGATGTACAAAAGTGTGATCTGGTGATGCTGCTTAAACGCTTTGGCa                                                                                                                  <  1:223192/223‑1 (MQ=255)                                                                      tctcGCCAAAATCGCCGCCGACATGAATAAACCCAACGGCCATTTTGTGACTACGCCGGCAGAAGTTCCGGCATGTTTACAAACCTTACCGCTGGCAAAAATCCTCGGCGGCGGCAAAGTCTCAGCTGCAAAACTGGAAGCGATGGGGCGGCGGACCTGCGATGATGTACAAGAGTGTGATCCGTGGATGCTTCTTAAACGCTTTGGCGAATTTGGCCGCATTTTGTGGGAGCGTAGTCAGGGGATTGAc                                                                         <  1:117406/250‑1 (MQ=255)                                                                          gCCAAAATCGCCTCCGACATGAATAAACCCAACGGCCAGTTTGTGATTACGCCGGCAGAAGTTCCGGCATTTTTACAAACCTTACCGCTGGCAAAAATCCCCGGCGTCGGCAAAGTCTCAGCGGCAAAACTGGAAGCGATGGGGCTTCGGACCTGCGATGATGTACAAAAGTGTGATCTGGTGATGCTGCTTAAACGCTTTg                                                                                                                     <  1:164605/202‑1 (MQ=255)                                                                                    ncTCCGACATGAATAAACCCAACGGCCAGTTTGTGATTACGCCGGCAGAAGTTCCGGCATTTTTACAAACCTTACCGCTGGCAAAAATCCCCGGCGTCGGCAAAGTCTCAGCGGCAAAACTGGAAGCGCTGGGGCTGCGGACCTGCGATGATGTACAAAAGTGTGATCTGGTGATGCTGCTTAAAc                                                                                                                           >  1:23229/2‑186 (MQ=255)                                                                                        cGACATGAATAAACCCAACGGCCAGTTTGTGATTACCCCGGCAGAAGTTCCGGCCTTTTTACAAACCTTACCGCTGGCAACAATCCCCGGCGACGGCAAACTCTCAGCGGCAAAACTGGAAGCGATGGGGCTGCGGACCTGCGATGATGTACAAAAGTGTGATCTGGTGATGCTGCTTAAACGCTTTGGCAAATTTGGCCGCATTTTGTGGGAGCGTAGTCAGGGGATTGACGAACGCGATGTTAACAGc                                                       >  1:27414/1‑250 (MQ=255)                                                                                                     cccAACGGCCAGTTTGTGATTACGCCGGCCGAAGTTCCGGCCTTTTTACAAACCTTCCCGCTGGCAACAATCCCCGGCGAAGGAAAAGTCTCAGCGGCCAAACTGGACGCGATGGGGCTGCGGACCTGCGCTGATGTACAAAAGTGTGATCTGGTGATGCTGCTTAAAAGCTTTGGCAAATTTGGCCGCATTTTGAGGGAGCGTAGTCAGGGGATTGACGAACGCGATGTTAACAGCGAACGGTTGCGaa                                          >  1:222768/1‑250 (MQ=255)                                                                                                                    gtgATTACGCCGGCAGAAGGTCCGGCATTTTTACAAACCTTACCGCTGGCAAAAATCCCCGGCGTCGGCAAAGTCTCAGCGGCAAAACTGGAAGCGATGGGGCTGCGGACCTGCGATGATGTACAAAAGTGTGATCTGGTGATGCTGCTTAAACGCTTTGTCAAATTGGGCCGCATTTTGTGTTAGCGTAGTCAGGGGATGGACGAACGCGATGTTAACAGCGAACGGTTGCGAAAATCCTTCGGCGTgg                           <  1:162863/250‑1 (MQ=255)                                                                                                                                gCAGAAGTTCCGGCATTTTTACAAACCTTACCGCTGGCACAAATCCCCGCCGTAGGCAAAGTATCAGCGGCAAAACTGGAAGCGATGGGGCTGCGGACCTGCGATGATGTACAAAAGTGTGATCTGGTGATGCTGCTTAAACGCTTTGGCAAATTTGGCCGCATTTTGTGGGAGCGTAGTCAGGGGATTGACGAACGCGATGTTAACAGCGAACGGTTGCGAAAATCCGTCGGCGTGGAACGCACGATGGc              >  1:165245/1‑251 (MQ=255)                                                                                                                                            gCATTTTTACAAACCGTACCGCTGGCAAAAATCCCCGGCGTCGGCAAAGTCTCAGCGGCAAACCTGGAAGCGATGGGGCTGCGGACCTGCGATGATGTACAAATGTGTGATCTGGTGATGCTGCTTAAACGCTTTGGCAAATTTGGCCGCATTTTGTGGGAGCTTAGTCAGGGGATTGACGAACGCGGTGTTAACAGCGAACGGTGGCGAAAATCCGTCGGCGTGGAACGCACGATGGCGGAAGATATtca  <  1:119950/251‑1 (MQ=255)                                                                                                                                                                  tGGCAAAAATCCCCGGCGTCGGCAAAGTCTCAGCGGCAAAACTGGAAGCGATGGGGCTGCGGACCTGCGATGATGTACAAAAGTGTGATCTGGTGATGCTGCTTAAACGCTTTGGCAAATTTGGCCGCATTTTGTGGGAGc                                                                                          <  1:86795/141‑1 (MQ=255)                                                                                                                                                                                                  gCGGCAAAACTGGAAGCGATGGGGCTGCGGACCTGCGATGATGTACAAAAGTGTGATCTGGTGATGCTGCTTAAACGCTTTGGCAAATTTGGCCGCATTTTGTGGGAGCGTCGTCAGGGGATTGACGAACGCGATGTTAACAGCGAACg                                                  >  1:221263/1‑149 (MQ=255)                                                                                                                                                                                                                                       |                                                                                                                                                                GGAAATCCGCCAGACAATCTTCAACGAGCTGCAACTGACGGCGTCTGCGGGCGTGGCACCAGTAAAGTTTCTCGCCAAAATCGCCTCCGACATGAATAAACCCAACGGCCAGTTTGTGATTACGCCGGCAGAAGTTCCGGCATTTTTACAAACCTTACCGCTGGCAAAAATCCCCGGCGTCGGCAAAGTCTCAGCGGCAAAACTGGAAGCGATGGGGCTGCGGACCTGCGGTGATGTACAAAAGTGTGATCTGGTGATGCTGCTTAAACGCTTTGGCAAATTTGGCCGCATTTTGTGGGAGCGTAGTCAGGGGATTGACGAACGCGATGTTAACAGCGAACGGTTGCGAAAATCCGTCGGCGTGGAACGCACGATGGCGGAAGATATTCA  >  NC_000913/251269‑251658

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑