Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	905,521	C→T	Y203Y (TAC→TAT)	amiD →	N‑acetylmuramoyl‑L‑alanine amidase D

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	905,521	0	C	T	100.0%	24.2 / NA	11	Y203Y (TAC→TAT)	amiD	N‑acetylmuramoyl‑L‑alanine amidase D
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (4/7);  total (4/7)

GCCGGAGTGAAATATTTTGCCCCGTTTGAACCGGCACAGATTCAGGCGCTTATTCCACTGGCGAAAGATATTATTGCCCGTTATCACATCAAGCCGGAAAACGTAGTGGCACATGCGGATATCGCACCGCAGCGCAAAGACGATCCGGGGCCATTATTTCCCTGGCAGCAACTGGCGCAGCAGGGGATTGGTGCCTGGCCGGATGCGCAGCGGGTTAACTTTTACCTTGCCGGGCGCGCGCCGCACACACCGGTAGATACCGCGTCATTGCTGGAGCTTTTGGCGCGCTACGGTTATGACGTTAAACCTGATATGACACCGCGCGAGCAGCGGCGCGTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCG  >  NC_000913/905297‑905766                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                      gCCGGAGTGAAATATTTTGCCCCGTTTGAACCGGCACCGATTCAGGCGCTTATTCCACTGGCGAAAGATATTATTGCCCGTTATCACATCAAGCCGGAAAACGTAGTGGCACATGCGGATATCGCACCGCAGCGCAAAGACGATCCGGGGCCATTATTTCCCTGGCAGCAACTGGCGCAGCAGGGGATTGGTGCCTGGCCGGATGCGCAGCGGGTTAACTTTTATCTTGCCGGGCGCGCGCCGCACACAcc                                                                                                                                                                                                                             >  1:191361/1‑251 (MQ=255)    ggAGTGAAATATTTTGCCCCGTTTGAACCGGCACAGATTCAGGCGCTTATTCCACTGGCGAAAGATATTATTGCCCGTTATCACATCAAGCCGGAAAACGTAGTGGCACATGCGGATATCGCACCGCAGCGCAAAGACGATCCGGGGCCATTATTTCCCTGGCAGCAACTGGCGCAGCAGGGGATTGGTGCCTGGCCGGATGCGCAGCGGGTTAACTTTTATCTTGCCGGGCGCGCGCCGCACACACCggn                                                                                                                                                                                                                          <  1:129151/251‑2 (MQ=255)                                                         aCTGGCGTAAGATATTATTGCCCGTTATCACATCAAGCCGGAAAACGTAGTGGCACATGCGGATATCGCACCGCAGCGCTGAGACGATCCGGGGCCATTATTTCCCTGGCAGCAACTGGCGCAGCAGGGGATTGGTGCCTGGCCTGATGCGCAGCGGGTTAACTTGTATCTTGCCTGGCTCGCGCCGCACACACCGGTAGTTACCGCGTCATTGCTGGAGCTTTTGGCGCGCTACGGTTATGACGTTAAAc                                                                                                                                                                     <  1:92772/251‑1 (MQ=255)                                                                                                                          tCGCACCGCGGCGCAAAGACGATCCGGGGCCATGATTTCCCTGGCAGCAACTGGCGCAGCAGGGGAGTGGTGCCTGGCCGGATGCGCAGCGGGTTAACTTTTATCTTGCCGGGCGCGCGCCGCACACACCGGTAGATCCCGCGTCATTGCTGGAGCTTTTGGCGCGCTACGGGCATGACGTTAAACCTGATATGACACCGCGCGAGCAGCGGCGCGTGATCATGGCATTc                                                                                                                         <  1:64948/230‑1 (MQ=255)                                                                                                                            ncACCGCAGCGCAAAGACGATCCGGGGCCATTATTTCCCTGGCAGCAACTGGCGCAGCAGGGGATTGGTGCCTGGCCGGATGCGCAGCGGGTTAACTTTTATCTTGCCGGGCGCGCGCCGCACACACCGGTAGATGCCGCGTCATTGCTGGAGCTTTTGGCGCGCTACGGTTATGACGTTAAACCTGATATGACACCGCGCGAGCAGCGGCGCGTGATTATGGCATTCCAGATGCATTTcc                                                                                                            >  1:38740/2‑241 (MQ=255)                                                                                                                                   aGCGCAAAGACGATCCGGGGCCATTATTTCCCTGTCAGCAACTGGCGCAGCAGGGGAGTGGTGCCTGGCCGGACGCGCAGCGGGTTAACTTTTATCTTGCCGGCCGCGCGCCGCACACACCGGTAGATACCGCGTCATTGCTGGAGCTTTTGGCGCGCTACGGTTATGACGTTAAACCTGATATGTCTCCGCGCGAGCAGCGGCGCGTGATTATGTCATTCCAGATGCATTTCCGCCCGACGTTATATaan                                                                                           <  1:19031/251‑2 (MQ=255)                                                                                                                                                            attTCCCTGGCAGCAACTGGCGCAGCAGGGGATTGGTGCCTGGCCGGATGCGCAGCTGGTTAACTTTTATCTTGCCGGGCGCGCGCCGCACACACCGGTAGATACCGCGTCATTGCTGGAGCTTTTGGCGCGCTACGGTTATGACGTTAAACCTGATATGACACCGCGCGAGCAGCGGCGCGTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCan                                                                   <  1:129785/250‑2 (MQ=255)                                                                                                                                                                                               gTGCCTGGCCGGATGAGCAGGGGGTTAACTTTTATCTTGACGGGCGCGCGCCGCACACACCGGTAGATACCGCGTCATTGCGGGAGGTTTTGGCGCGCTACGGTTATGACGTTAAACCTGATATGACACCGCGNGAGNNnnngcnnnnnn                                                                                                                                    >  2:136777/1‑144 (MQ=255)                                                                                                                                                                                                     ggCCGGATGCGCAGCGGGTTAACTTTTATCTTGCCGGGCGCGCGCCGCACACACCGGTAGATACCGCGTCATTGCTGGAGCTTTTGgcn                                                                                                                                                                                           <  1:27151/89‑2 (MQ=255)                                                                                                                                                                                                      gCCGGATGCGCAGCGGGTTAACTTTTATCTTGCCGGGCGCGCGCCGCACACCCCGGTAGATACCGCGTCATTGCTGGAGCTTTTGGCGCGCTACGGTTATGACGTTAAAACTGATATGACACCGCGCGAGCAGCGGCGCGCGATTATCGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGACGCCTTGCTGGAGAAATACGGGCAGGATTAgc                        >  1:118328/1‑251 (MQ=255)                                                                                                                                                                                                                             tttATCTTGCCGGGCGCGCGCCGCACACACCGGTAGATACCGCGTCATTGCTGGAGCTTTTGGCGCGCTACGGTTATGACGTTAAACCTGATATGACACCGCGCGAGCAGCGGCGGGTGATTATTGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCTAAGCGGATGCAGAAACTCAGGCTATTTCCGAAGCATTGCTGGAGAAGTACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTcgcn  <  1:41010/250‑2 (MQ=255)                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                      GCCGGAGTGAAATATTTTGCCCCGTTTGAACCGGCACAGATTCAGGCGCTTATTCCACTGGCGAAAGATATTATTGCCCGTTATCACATCAAGCCGGAAAACGTAGTGGCACATGCGGATATCGCACCGCAGCGCAAAGACGATCCGGGGCCATTATTTCCCTGGCAGCAACTGGCGCAGCAGGGGATTGGTGCCTGGCCGGATGCGCAGCGGGTTAACTTTTACCTTGCCGGGCGCGCGCCGCACACACCGGTAGATACCGCGTCATTGCTGGAGCTTTTGGCGCGCTACGGTTATGACGTTAAACCTGATATGACACCGCGCGAGCAGCGGCGCGTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCG  >  NC_000913/905297‑905766

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑