Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	899,899	G→A	A226V (GCA→GTA)	artJ ←	L‑arginine ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	899,899	0	G	A	100.0%	13.1 / NA	6	A226V (GCA→GTA)	artJ	L‑arginine ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (2/4);  total (2/4)

CGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTACCTTTGTCATCAGTCTCAAGCCGCGGTTGCGGCTTTCTGAATCTTACTGTGGGAACCACTGGTCACTGATTTTTTGATAAGTACCGTCAGCTTTAATTGCTGCCAGCGCGTTATTCAGTTTTTCCAGCAGGGCTTTGTTATCCGGACGTACAGCGATGCCCAGGCCGGTGCCAAAATATTGCGGATCGGTCACTTTCTCAGTAGCAACACCAAGTTGTGGATTGGTTTTCAGCCATTCGTTT  >  NC_000913/899684‑900042                                                                                                                                                                                                                        |                                                                                                                                                cgcgTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTACCTTTGTCATCATTCTCAAGCCGCGGTTGCGGCTTTCTGAATCTTACTGTGGGAACCACTGGTCACTGATTTTTTGATAAGTTCCGTCAGCTTTAATTACTGCCAGCGCGTTATTCAGTTTTTCCAGCAGGGCt                                                                                                              <  1:86589/251‑1 (MQ=255)                                                  aGCGTGCAAATTCAATACATTGCATGGGCCATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTACCTTTGTCATCAGTCTCAAGCCGCGGTTGCGGCTTTCTGAATCTTACTGTGGGAACCACTGGTCACTGATTTTTTGATAATTACCGTCAGCTTGAATTACTGCCTGCGCGTTATTCAGTTTTTCCAGCAGGGCTTTGTTATCCGTTCGTACAGCGATGCCCAGGCCGGTGCCAAAATATTGCg                                                             <  1:161978/251‑1 (MQ=255)                                                                    agtGCATGGGCCATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCCTTTGTCATCAGTCTCAAGCCGCGGTTGCGGCTTTCTGAATCTTACTGTGGGAACCACTGGTCACTGATTTTTTGATAAGTACCGTCAGCTTTAATTACGTCCATCGCGTTATTCAGTTTTTCCAGCAGGGCTTTGTTATCCGGACGTACAGCGATGCCCAGGCCTGTGCCAAAATATTACGGATCGGTCTCTTTCTCAg                                           <  1:165950/249‑1 (MQ=255)                                                                                                             gCAATTTTACCTTTGTCATCAGTCTCAAGCCGCGGTTGCGGCTTTCTGAATCTTACTGTGGGAACCACTGGTCACTGATTTTTTGATAAGTACCGTCAGCTTTAATTACTGCCAGCGCGTTATTCAGTTTTTCCAGCAGGGCTTTGTTATCCGGACGTACAGCGATGCCCAGGCCGGTGCCAAAATATTGCGGATCGGTCACTTTCTCAGTAGCAACACAAAGTTGTGGATGGGTTGTCAGCCATTCGttg  <  1:62459/251‑2 (MQ=255)                                                                                                                                caGTCTCAAGCCGCGGTTGCGGCTTTCTGAATCTTACTGTGGGAACCACTGGTCGCTGATTTTTTGATAAGTACCGTCAGCTTTCATTACTGCCAGCGCGTTATTCAGTTTTTCCAGCAGGGCTTTGTTATCCGGACGTACAGCGCTGCCCAGGCCGGTGCCAAAATATTGCGGATCGGTCACTTTCTCAGTAGCAACACCACGTTGCGGATTGGTTTTCa             >  1:158052/1‑221 (MQ=255)                                                                                                                                               gTTGCGGCTTTCTGCATCTTACTGTGGGAACCACTGGTAAATGATTTTTTGATAAGTACCGTCAGCTTTAATTACTGCCAGCGCGTTATTCAGTTTTTCCAGCAGGGCTTTGTTATCCGGACGTACAGCGATGCCCAGGCCGGTGCCAAAATATTGCGGATCGGTCACTTTCTCAGTAGCAACACCAAGTTGTGGATTGGTTTTCAGc           >  1:185113/1‑208 (MQ=255)                                                                                                                                                                                                                        |                                                                                                                                                CGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTACCTTTGTCATCAGTCTCAAGCCGCGGTTGCGGCTTTCTGAATCTTACTGTGGGAACCACTGGTCACTGATTTTTTGATAAGTACCGTCAGCTTTAATTGCTGCCAGCGCGTTATTCAGTTTTTCCAGCAGGGCTTTGTTATCCGGACGTACAGCGATGCCCAGGCCGGTGCCAAAATATTGCGGATCGGTCACTTTCTCAGTAGCAACACCAAGTTGTGGATTGGTTTTCAGCCATTCGTTT  >  NC_000913/899684‑900042

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑