Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	870,836	C→T	P476S (CCG→TCG)	gsiB →	glutathione ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	870,836	0	C	T	100.0%	16.8 / NA	8	P476S (CCG→TCG)	gsiB	glutathione ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (2/6);  total (2/6)

TCTACACTGGCTGGTCGGCTTCAACCGGCGAAGCGGACTGGGCACTATCGCCGCTGTTTGCCTCGCAGAACTGGCCACCGACGCTGTTTAATACCGCGTTTTACAGCAATAAACAGGTGGATGACTTCCTGGCTCAGGCACTGAAAACTAATGATCCGGCGGAAAAGACCCGCTTATATAAGGCGGCGCAGGATATCATCTGGCAAGAATCGCCGTGGATCCCGCTGGTGGTAGAAAAACTGGTGTCGGCACACAGTAAAAACCTGACCGGTTTTTGGATCATGCCAGACACCGGCTTCAGCTTTGAAGACGCGGATTTGCAATAAGCAACGCAGGGAGTGGAATGCTTAATTACGTTATCAAACGCTTACTGGGGTTGATTCCGACGCTGTTTATCGTCTCGGTGCTGGTGTTTTTATTTGTCCATATGCTGCCCGGCGATCCGGC  >  NC_000913/870624‑871070                                                                                                                                                                                                                     |                                                                                                                                                                                                                                           tCTACACTGGCTGGTCGGCTTCAACCGGCGAAGAGGAATGGGCACTATCGCCGCTGTTTGCCTCGCAGAGCTGGCCACCGACGCTGTTTAATACCGCGTTTTACAGCAATAAACAGGTGGATGACTGCCGGGCTCAGGCACTGAAAACTAATGACCCGGCGGAAAAGACCCGCTTATATAAGGCGGCGTAGGATATCATCTGGCAAGAATCGTCGTGGATCCCGCTGGTGGTAGAAAAACTGGTGTCggn                                                                                                                                                                                                       <  1:19439/250‑2 (MQ=255)                             cGAAGCGGACTGGGCACTATCGCCGCTGTTTGCCTCGCAGAACTGGCCACCGACGCTGTTTAATACCGCGTTTTACAGCAATAAACAGGTGGATGACTTCCTGGCTCAGGCACTGAAAACTAATTAGCCGGCGGAAAAGACCCGCTTATATAAGGCGGCGCAGGATATCATCTGGCAAGAATCGTCGTGGATCCCGCTGGTGGTAGAAAAACTGGTGTCGGCACACAGTAAAAACCTGACCGGTTTTTGGa                                                                                                                                                                          <  1:103004/251‑1 (MQ=255)                                  cGGACTGGGCACTATCGCCGCTGTTTGCCTCGCAGAAATGGCCACCGCCGATGTTTAATACCGCGTTTTACAGCAATAAACAGGTGGATGACTTCCTGGCTCAGGCACTGAAAACTAATGATCCGGCGGAAAAGACCCGCTTATATAAGGCGGCGCAGGATATCATCTGGCAAGAATCGTCGTGGATCCCGCTGGTGGTAGAAAAACTGGTGTCGTCACACAGTAAAAACCTTACCGGGTTTTGGATCANg                                                                                                                                                                     <  1:170735/251‑1 (MQ=255)                                                                                                 cgTTTTACAGCAATAAACAGGTGGATGACTTCCTGGCTCAGGCACTGAAAACTAATGATCCGGCGGAAAAGACCCGCTTATATAAGGCGGCGCAGGATATCATCTGGCAAGAATCGTCGAGGATCCCGCTGGTGGTAGAAAAACTGGTGTCGGCACACAGTAAAAACCTGACCGGTTTTTGGATCATGCCAGACACCGGCTTCAGCTTTGAAGACGCGGATTTGCAATAAGCAACGCAGGGAGTGGAATGc                                                                                                      <  1:76511/251‑1 (MQ=255)                                                                                                           cAATAAACAGGTGGATGACTTCCTGGCTCAGGCACTGCAAACTAATGATCCGGCGGACAAGACCCGCTTATATAAGGCGGCGCAGGATATCATCTGGCAAGAATCGTCGTGGATCCCGCTGGTGGTAGAAAAACTGGTGTCGGCACACAGTAAAAACCTGACCGGTTTTTGGATCATGCCAGACACCGGCTTCAGCTTTGAAGACGCGGATTTGCAATAAGCAACGCAGGGAGTGGAAt                                                                                                        >  1:80368/1‑239 (MQ=255)                                                                                                                                                                                          gcgcAGGATATCATCTGGCAAGAATCGTCGTGGATCCCGCTGGTGGTAGAAACAATGGTGTCGGCACACAGTACAAACCTGACCGGTTTTTGGACCATGCCAGACACCGGCTTCAGCTTTGCAGACGCGGATTTGCAATAAGCAACGCAGGGAGTGGAATACTTACTTACGTTATCAAACGCTTACTGGGGTTGATTCCGACGATGTTTATCGTCTCGGTGCTGGTGTTTTTATTTGTCCATATGCTGccc             >  1:111878/1‑251 (MQ=255)                                                                                                                                                                                             cAGGATATCATCTGGCAAGAATCGTCGTGGATCCCGCTGGTGGTAGAAAAACTGGTGTCGGCACACAGTAAAAACCTGACCGGTTTTTGGATCATGCCAGACACCGGCTTCAGCTTTGAAGACGCGGATTTGCAATAAGCAACGCAGGGAGTGGAATGCTTAATTACGTTATCAAACGCTTACTGGGGTTGATTCCGACGCTGTTTATCGTCTCGGTGCTGGTGTTTTTATTTGGCCAGATGCTTCCCGGc          <  1:78335/251‑1 (MQ=255)                                                                                                                                                                                                     catcTGGCAAGAATCGTCGTGGATCCCGCTGGTGGTAGAAAAACTGGTGTCGGCACACAGTAAAAACCTGACCGGTTTTTGGATCATGCCAGACACCGGCTTCAGCTTTGAAGACGCGGATTTGCAATAAGCAACGCAGGGAGTGGAATGCTTAATTACGTTATCAAACGCTTACTGGGGTTGATTCCGACGCTGTTTATCGTCTCGGTGCTGGTGTTTTTATTTGTCCATATGCTGCCCGGCGATCCGgc  <  1:94422/251‑1 (MQ=255)                                                                                                                                                                                                                     |                                                                                                                                                                                                                                           TCTACACTGGCTGGTCGGCTTCAACCGGCGAAGCGGACTGGGCACTATCGCCGCTGTTTGCCTCGCAGAACTGGCCACCGACGCTGTTTAATACCGCGTTTTACAGCAATAAACAGGTGGATGACTTCCTGGCTCAGGCACTGAAAACTAATGATCCGGCGGAAAAGACCCGCTTATATAAGGCGGCGCAGGATATCATCTGGCAAGAATCGCCGTGGATCCCGCTGGTGGTAGAAAAACTGGTGTCGGCACACAGTAAAAACCTGACCGGTTTTTGGATCATGCCAGACACCGGCTTCAGCTTTGAAGACGCGGATTTGCAATAAGCAACGCAGGGAGTGGAATGCTTAATTACGTTATCAAACGCTTACTGGGGTTGATTCCGACGCTGTTTATCGTCTCGGTGCTGGTGTTTTTATTTGTCCATATGCTGCCCGGCGATCCGGC  >  NC_000913/870624‑871070

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑