Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	866,113	C→T	Q84Q (CAG→CAA)	moeA ←	molybdopterin molybdotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	866,113	0	C	T	100.0%	21.8 / NA	10	Q84Q (CAG→CAA)	moeA	molybdopterin molybdotransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (6/4);  total (6/4)

GTCAGGCGAGTTCCCGCCGGGAAAACAACCGCACCTGCAGAGATATCTTCACCGCGACGGCGAATATTTTGCCCGCTACGCACTTCAGCAGTAAAACGCACGCCATTGTCCATTTGTTCAGTCTGCTCCTGCATCACCACCGCTTCGCAGCCTTCCGGCACCGGCGCACCGGTCATAATACGAATGCAGGTACCCGCAGGCCATTCACCATGGTATGGCTGACCGGCAAAGGATTTACCGGCAACGGGCAGCGGTTGCCCGGAGGCAATATCGGCTAAACGCACCGCGTAGCCGTCCATTGCGGAGTTATCAAACCCCGGAACATCAAGCGGCGAAACGACATCGCTCGCCAGAATACGACCAAAACACTGTACCAGTGGCAGCGTTTCCTGGGCGGTC  >  NC_000913/865895‑866293                                                                                                                                                                                                                           |                                                                                                                                                                                     gTCAGGCGAGTTCCCGCCGGGAAAACAACCGCACCTGCAGAGATATCTTCACCGCGACGGCGAATATTTTGCCCGCTACGCACTTCAGCAGTAAAACGCACGCCATTGTCCATTTGTTCAGTCTGCTCCTGCATCACCACCGCTTCGCAGCCTTCCGGCACCGGCGCACCGGTCATAATACGAATGCAGGTACCCGCAGGCCATTCACCATGGTATGGTTGACCGGCAAAGGATTTACCGGCAACGGGc                                                                                                                                                        >  1:4573/1‑249 (MQ=255)  tCAGGCGAGTTCCCGCCGGGAAAACAACCGCACCTGCAGAGATATCTTCACCGCGACGGCGAATATTTTGCCCGCTACGCACTTCAGCAGTAAAACGCACGCCATTGTCCATTTGTTCAGTCTGCTCCTGCATCACCACCGCTTCGCAGCCTTCCGGCACCGGCGCACCGGTCATAATACGAATGCAGGTACCCGCAGGCCATTCACCATGGTATGGTTGACCGGCAAAGGATTTACCGGCAACGGGCAGc                                                                                                                                                     >  1:7689/1‑251 (MQ=255)      gCGAGTTCCCGCCGGGAAAACAACCGCACCTGCAGAGATATCTTCACCGCGACGGCGAATATTTTGCCAGCTACGCCCTTCAGCAGTAAAACGCACGCCCTTGTCCATTTGTTCAGTCTGCTCCTGCATCACCACCGCTTCGCAGCCTTCCGGCACCGGCGCACCGGTCATAATACGAATGCAGGTACCCGCAGGCCATTCACCATGGTATGGTTGACCGGCAAAGGATTTACCGGCAACGGGCAGCGGtt                                                                                                                                                 >  1:147051/1‑251 (MQ=255)                                                  cACCGCGACGGCGAATATTTTGCCCGCTACGCACTTCAGCAGTAAAACGCACGCCATTGTCCATTTATTCAGTCTGGTCCTGCATCACAACCGCTTCGCAGCCTTCCGGCACCGGCGCACCGGTCATAATACGAATGCAGGTACCCGCAGGCCATTCACCATGGTATGGTTGACCGGCAAAGGATTTACCGGCAACGGGCAGCGGTTGCCCGGAGGCAATATCGGCTACACGCCCCGCGTAGCCg                                                                                                           >  1:148962/1‑245 (MQ=255)                                                        gACGGCGAATATTTTGCCCGCTACGCACTTCAGCAGTAAACCGCACGCCATTTTCCATTTGTTCAGTCTGCTCCTGCATCACCACCGCTTCGCAGCCTTCCGGCACCGGCGCACCGGTCATAATACGAATGCAGGTACCCGCAGGCCATGCACCATGGTATGGTTGACCGGCAAAGGATTTACCGGCAACGGGCAGCGGTTGCCCGGAGGCAATATCGGCTAAACGCACCGCGTAGCCGTCCATTGCGGAg                                                                                               <  1:159915/251‑1 (MQ=255)                                                                                                                          tCTGCTCCTGCATCACCACCGCTTCGCAGCCTTCCGGCACCGGCGCACCGGTCCTAATACGAATGCAGGTACCCGCAGGCCCTTCACCATGGAATGGTTGACCGGCAAAGGATTTACCGGCAACGGGCAGCGGTTGCCCGACGGCAATATCGGCTAAACGCACCGCGTAGCCGTCCATTGCGGAGTTATCAAACCCCGGAACATCAAGCGGCGAAACGACATCGCTCGCCAGAATACGACCAAAACACTGt                             >  1:118733/1‑251 (MQ=255)                                                                                                                                       caccacCGCTTCGCAGCCTTCCGGCACCGGCGCACCGGTCATAATACGAATGCAGGTACCCGCAGGCCATTCACCATGGTATGGTTGACCGGCAAAGGATTTACCGGCAACGGGCAGCGGTTGCCCGGAGGCAATATCGGCTAAACGCACCGCGTAGCCGTCCATTGCGGAGTTATCAAACCCCGGAACATCAAGCGGCGAAACGACATCGCTCGCCAGAATACGACCAAAACACTGTACCAGTGGCAGc                 <  1:61278/250‑1 (MQ=255)                                                                                                                                           accGCTTCGCAGCCTTCCGGCACCGGCGCACCGGTCATAATACGAATGCAGGTACCCGCAGGCCATTCACCATGGTATGGTTGACCGGCAAAGGATTTACCTGCAACGGGCAGCGGTTGCCCGGAgg                                                                                                                                        <  1:91611/127‑1 (MQ=255)                                                                                                                                                  cGCAGCCTTCCGGCACCGGCGCACCGGTCATAATACGAATGCAGGTACCCGCAGGCCATTCACCATGGTATGGTTGACCGGCAAAGGATTTACCGGCAACGGGCAGCTGTTGCCCGGAGGCAATATCGGCTAAACTCACCTCGTAGCCGTCCATTGCGGAGTTATCAAACCCCGGAACATCAAGCGGCGAAACGACATCGCTCGCCAGAATACGAc                                        <  1:35034/216‑1 (MQ=255)                                                                                                                                                      gCCTTCCGGCACCGGCGCACCGGTCATAATACGAATGCAGGTACCCGCAGGCCATTCACCATGGTATGGTTGACCGGCAAAGGATTTACCGGCAACGGGCAGCGGTTGCCCGGAGGCAATATCGGCTAAACGCACCGCGTAGCCGTCCATTGCGGAGTTATCAAACCCCGGAACATCAAGCGGCGAAACGACATCGCTCGCCAGAATACGACCAAAACACTGTACCAGTGGCAGCGTTTCCTGGGCGGTc  >  1:107458/1‑250 (MQ=255)                                                                                                                                                                                                                           |                                                                                                                                                                                     GTCAGGCGAGTTCCCGCCGGGAAAACAACCGCACCTGCAGAGATATCTTCACCGCGACGGCGAATATTTTGCCCGCTACGCACTTCAGCAGTAAAACGCACGCCATTGTCCATTTGTTCAGTCTGCTCCTGCATCACCACCGCTTCGCAGCCTTCCGGCACCGGCGCACCGGTCATAATACGAATGCAGGTACCCGCAGGCCATTCACCATGGTATGGCTGACCGGCAAAGGATTTACCGGCAACGGGCAGCGGTTGCCCGGAGGCAATATCGGCTAAACGCACCGCGTAGCCGTCCATTGCGGAGTTATCAAACCCCGGAACATCAAGCGGCGAAACGACATCGCTCGCCAGAATACGACCAAAACACTGTACCAGTGGCAGCGTTTCCTGGGCGGTC  >  NC_000913/865895‑866293

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑