Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	804,325	C→T	Q275* (CAG→TAG)	ybhJ →	putative hydratase YbhJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	804,325	0	C	T	100.0%	17.7 / NA	8	Q275* (CAG→TAG)	ybhJ	putative hydratase YbhJ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (4/4);  total (4/4)

GTGTTCAAAAACGGTTACGTCAAAAACAAAGTCATGGAGTTCGTTGGACCGGGCGTTAGCGCGCTCTCTACCGATTTCCGTAACAGCGTTGACGTGATGACCACTGAAACGACCTGTTTAAGTTCTGTCTGGCAAACCGATGAAGAAGTCCATAACTGGCTGGCGCTGCACGGTCGCGGCCAGGATTACTGCCAGCTTAACCCTCAACCGATGGCGTACTACGATGGCTGCATCAGCGTTGATTTAAGCGCCATCAAACCAATGATTGCGCTGCCGTTCCACCCGAGCAACGTGTATGAAATCGACACACTGAACCAGAACCTGACCGACATTCTGCGTGAGATTGAAATTGAGTCCGAACGCGTGGCGCACGGTAAAGCCAAACTCTCGCTGCTGGATAAAGTGGAAAATGGTCGCCTGAAAGTGCAGC  >  NC_000913/804133‑804562                                                                                                                                                                                                 |                                                                                                                                                                                                                                              gtgtTCAAAAACGGTTACGTCAAAAACAAAGTCATGGAGTTCGTTGGACCGGGCGTTAGCGCGCTCTCTACCGATTTCCGTAACAGCGTTGACGTGATGACCACTGAAACGACCTGTTTAAGTTCTGTCTGGCAAACCGATGAAGAAGTCCATAACTGGCTGGCGCTGCACGGTCGCGGCCAGGATTACTGCTAGCTTAACCCTCAACCGATGGCGTACTACGATggn                                                                                                                                                                                                            <  1:135788/228‑2 (MQ=255)                                                      cGTTAGCGCGCTCTCTACCGCTTTCCGTAACAGCGTTGACGTGATGACCACTGAAACGACCTGTTTAAGTTCTGTCTGGCAAACCGATGAAGAAGTCCATAACTGGCTGGCGCTGCACGGTCGCGGCCAGGATTACTGCTAGCTTAACCCTCAACCGATGGCGTACTACGATGGCTGCATCAGCGTTGATTTAAGCGCCATCAAACCAATGATTGCGCTGCCGTTCCACCCGAGCAACGTGTATGAAATCg                                                                                                                                >  1:167273/1‑251 (MQ=255)                                                                              ccGTAACAGCGGTGACGTGATGACCACTGAAACGACCTGTTTAAGTTCTGTCTGGCAAACCGATGAAGAAGTCCATAACTGGCTGGCGCTGCACGGTCGCGGCCAGGATTACTGCTAGCTTAACCCTCAACCGATGGCGTACTACGATGCCTGCATCAGCGTTGATTTAAGCGCCACCAAACCAATGATTGCGGTTTCGGCCCACCCGAGCAACGTGTATGAAATCGACACACTGAACCAGAACCTGaac                                                                                                         <  1:201494/250‑3 (MQ=255)                                                                                                                                                      ccATAACTGGCTGGCGCTGCACGGTCGCGGCCAGGATTACTGCTAGCTTAACCCTCAACCGATGGCGTACTACGATGGCTGCATCAGCGTTGATTTAAGCGCCATCAAACCAATGATTGCGCTGCCGTTCCACCCGAGCAACGTGTATGAAATCGACACACTGAACCAGAACCTGACCGACATTCTGCGTGAGATTGAAATTGAGTCCGAACGCGTGGCGCACGGTAAAGCCAAACTCTCGCTGCTGGAt                                 >  1:86588/1‑250 (MQ=255)                                                                                                                                                       naTAACTGGCTGGCGCTGCACGGTCGCGGCCAGGATTTCTGCTAGCTTAACCCTCAAGAGATGGCGCACTACGa                                                                                                                                                                                                                >  1:43566/2‑74 (MQ=255)                                                                                                                                                                          aCGGTCGCNGCCANGNNNACTGCNAGCTTAACCNTCAACCGATGGCGTACTACNATGGCt                                                                                                                                                                                                           >  2:22980/1‑60 (MQ=255)                                                                                                                                                                          aCGGTCGCGGCCAGGATTACTGCTAGCTTAACCCTCAACCGATGGCGTACTACGATGGCTGCATCAGCGTTGATTTAAGCGCCATCAAACCAATGATGGCGCTGCCGTTCCACCCGAGCAACGTGTATGAAATCGACACACTGAACCAGAACCTGACCGACATTCTGCGtn                                                                                            <  1:22980/171‑2 (MQ=255)                                                                                                                                                                                  ggCCAGGATTACGGCTAGCTTAACCCTCAACCGATGGCGTACTACGATGGCTGCATCAGCGTTGATTTAAGCGCCATCAAACCAATGATTGCGCTGCCGTTCCACCCGAGCAACGTGTATGAAATCGACACACTGAACCAGAACCTGACCGACATTCTGCGTGAGATTGAAATTGAGTCCGAACGCGTGGCGCACGGTAAAGCCAAACTCTCGCTGCTGGATAAAGTGGAAAATgg                   <  1:1748/236‑1 (MQ=255)                                                                                                                                                                                    ccAGGATTACTGCTAGCTTAACCCTCAACCGATGGCGTACTACGATGGCTGCATCAGCGTTGATTTAAGCGCCATCAAACCAATGATTGCGCTGCCGTTCCACCCGAGCAACGTGTATGAAATCGACACACTGAACCAGAACCTGACCGACATTCTGCGTGAGATTGAAATTGAGTCCGAACGCGTGGCGCACGGTAAAGCCAAACTCTCGCTGCTGGATAAAGTGGAAAATGGTCGCCTGAAAGTgcagc  >  1:151557/1‑251 (MQ=255)                                                                                                                                                                                                 |                                                                                                                                                                                                                                              GTGTTCAAAAACGGTTACGTCAAAAACAAAGTCATGGAGTTCGTTGGACCGGGCGTTAGCGCGCTCTCTACCGATTTCCGTAACAGCGTTGACGTGATGACCACTGAAACGACCTGTTTAAGTTCTGTCTGGCAAACCGATGAAGAAGTCCATAACTGGCTGGCGCTGCACGGTCGCGGCCAGGATTACTGCCAGCTTAACCCTCAACCGATGGCGTACTACGATGGCTGCATCAGCGTTGATTTAAGCGCCATCAAACCAATGATTGCGCTGCCGTTCCACCCGAGCAACGTGTATGAAATCGACACACTGAACCAGAACCTGACCGACATTCTGCGTGAGATTGAAATTGAGTCCGAACGCGTGGCGCACGGTAAAGCCAAACTCTCGCTGCTGGATAAAGTGGAAAATGGTCGCCTGAAAGTGCAGC  >  NC_000913/804133‑804562

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑