Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	802,954	C→T	N356N (AAC→AAT)	ybhI →	putative tricarboxylate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	802,954	0	C	T	100.0%	26.9 / NA	12	N356N (AAC→AAT)	ybhI	putative tricarboxylate transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (5/7);  total (5/7)

GTCTTTGTGCTGGCGCTGCTGGGCTGGATTTTCAGTAAGTCTCTGGGGGTTGATGAATCCACCGTGGCAATCGTTGTTATGGCAACCATGCTGCTGCTGGGTATCGTTACCTGGGAAGACGTGGTTAAAAATAAAGGCGGCTGGAATACCTTAATCTGGTACGGCGGTATTATCGGCTTAAGCTCCTTATTATCGAAAGTTAAATTCTTCGAATGGTTAGCTGAAGTCTTTAAAAATAACCTGGCATTTGATGGTCACGGTAACGTTGCTTTCTTCGTTATTATTTTCCTCAGCATTATCGTGCGTTATTTCTTCGCTTCCGGTAGTGCCTATATCGTTGCTATGTTACCGGTATTTGCCATGCTGGCGAACGTCTCCGGCGCACCGTTAATGTTAACCGCGCTGGCACTGTTGTTCTCCAACTCCTATGGCGGCATGGTTACTCACTATGGCGGCGCGGCAGGTCCGGTCATCTTTGGC  >  NC_000913/802715‑803194                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                 gtCTTTGTGCTGGCGCTGCTGGGCTGGATTTTCAGTAAGTCTCTGGGGGTTGATGAATCCACCGTGGCAATCGTTGTTATGGCAACCATGCTGCTGCTGGGTATCGTTACCTGGGAAGACGTGGTTAAAAATAAAGGCGGCTGGAATACCTTAATCTGGTACGGCGGTATTATCGGCTTAAGCTCCTTATTAGCGAAAGTTAAATTCTTCGAATGGTTAGCTGAAGTCTTTAAAAATAATCTGGCATTTg                                                                                                                                                                                                                                        <  1:91823/250‑1 (MQ=255)                                                ngTTGATGAATCCACCGTGGCAATCGTTGTTATGGCAACCATGCTGCTGCTGGGTATCGTTACCTGGGAAGACGTGGTTAAAAATAAAGGCGGCTGGAATACCTTAATCTGGTACGGCGGTATTATCGGCTTAAGCTCCTTCTTATCGAAAGTTAAATTCTTCGAATGGTTAGCTGAAGTCTTTAAAAATAATCTTGCATTTGATGGTCACGGGAAc                                                                                                                                                                                                                          >  1:131721/2‑217 (MQ=255)                                                                                                                         gTNNNNNAAANTAAAGGCGGCTGGAATACCTTAATCTGGTACGGCGGTATTATCGGCTTAAGCTCCTTATTATCGAAAGTTAAATTCTTCGAATGGTTAGCTGAAGTCTTTAAAAATAATCTGGCATTTGATGGTCACGGTAAc                                                                                                                                                                                                                          <  2:131721/144‑1 (MQ=255)                                                                                                                            nttAAAAATAAAGGCGGCTGGAATACCTTAATCTGGTACGGCGGTATTATCTGCTTAAGCTCCTTATTATCGAAAGTTAAATTCTTCGAATGGTTAGCTGAAGTCTTTAAAAATAATCTTGGATTTGATGGGCACGGTAACGTTTCTTTCTTCGTTATTTTTTTCCTCAGCATTATCGGGCGTTATTTCTTTGCTTCCGGGAGTGCCTATATCGTTGGTTTGTTTCCGGTATTTGGCATGCTGGCCAACGt                                                                                                            >  1:132353/2‑251 (MQ=255)                                                                                                                                             cTGGAATACCTTAATCTGGTACGGCGGAATTTTTGGCTTAAGGCCCTTTTTATTGAAAGTTAAATTCTTTGAATGGTTATCTGAAGTCTTTAAAAATAATCTGGCATTTTATGGTCACGGGAACGTTGCTTTTTTCGTTTTTATTTTTCTCATCATTATCGTGCGTTTTTTCTTTGCTTCCGGTAATGTCTATTTCGTTTCTTTTTTACCGGGCTTTGCCATGCTGGCGAACGTCTCCGGCGCCCCGttt                                                                                            >  1:10087/1‑249 (MQ=255)                                                                                                                                                              ggTACGGCGGTATGATCGGCTTAAGCTCCTTATTATCGAAATTTAAATTCTTCGAATGGTTAGCTGAAGTTTTTAAAAATAATCTGGCATTTGATGGTCACTGTAACGTTGCTTTCTTCGTTATTATTTTCCTCAGCATTATCGTGCGTTATTTGTTCGCTTCCGGTGGTGCCTATATCGTTGCTGTGTTACCGGTATATGCCATGCCGGCGAACGTCTACGGCGCACCGTTAATGTTAACCGCGCTggt                                                                           <  1:154360/250‑2 (MQ=255)                                                                                                                                                                            atCGGCTTAAGCTCCTTATTATCGAAAGTTAAATTCTTCGAATGGTTAGCTGAAGTCTTTAAAAATAATCTGGCATTTGATGGTCACGGTAACGTTGCTTTCTTCGTTATTATTTTCCTCAGCa                                                                                                                                                                                           >  1:10505/1‑124 (MQ=255)                                                                                                                                                                                                           aaTTCTTCGAATGGTTAGCTGAAGTCTTTAAAAATAATCTGGCATGTGATGGTCACGGTAACGTTGTTTTCTTCGTTATTATTTTCCTCAGCAGTATCGTGCGTTATTTCTTCGCTTCCGGTAGTGCCTAGATCGTTTCTATGTTTTCTGTATTGGCCATGCTGGCGAAGGTGTCCGGCGCACCGTTAAGGTTAACCGCGCTGGCACTGTTGGTCTCCAACTCCTATGGCGGCATGGTTACTCACTATggc                             <  1:112585/251‑1 (MQ=255)                                                                                                                                                                                                                   gAATGNTTAGCNGNNGTCTTTAAAACTAATCTGGNATTTGATGGTCACGGNAACGNTGCTtnctnc                                                                                                                                                                                                              >  2:92074/1‑66 (MQ=255)                                                                                                                                                                                                                        gttatgtGAACTCTTTAAAAATAATCAGGCATTTGAGTGTCACGGTAACGTTGCTTTCTTCGTTATTATTTTccc                                                                                                                                                                                                <  1:106138/69‑2 (MQ=255)                                                                                                                                                                                                                            gCTGAAGTCTTTAAAAATAATCTGGCATTTGATGGTCACGGTAACGTTGCTTTCTTCGTTATTATTTTCCTCAGCATTATCGTGCGTTATTTCTTCGCTTCCGGTAGTGCCTATATCGTTGCTATGTTACCGGTATTTGCCATGCTGGCGAACGTCTCCGGCGCACCGTTAATGTTAACCGCGCTGGCACTGTTGTTCTCCAACTCCTATGGCGGCATGGTTATTCTCTATGGCGGCGCGGCAGGTCCGGt            <  1:168839/251‑1 (MQ=255)                                                                                                                                                                                                                                       tAAAAATAATCTGGCATTTGATGGTCACGGTAACGTTGCTTTCTTCGTTATTATTTTCCTCAGCATTATCGTGCGTTATTTCTTCGCTTCCGGTAGTGCCTATATCGTTGCTATGTTCCCGGTATTTGCCATGCTGGCGAACGTCTCCGGCGCACCGTTAATGTTAACCGCGCTGGCACTGTTGTTCTCCAACTCCTATGGCGGCATGGTTACTCACTATGGCGGCGCGGCAGTTCCGGTCATCTTTGGc  <  1:49644/250‑1 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                 GTCTTTGTGCTGGCGCTGCTGGGCTGGATTTTCAGTAAGTCTCTGGGGGTTGATGAATCCACCGTGGCAATCGTTGTTATGGCAACCATGCTGCTGCTGGGTATCGTTACCTGGGAAGACGTGGTTAAAAATAAAGGCGGCTGGAATACCTTAATCTGGTACGGCGGTATTATCGGCTTAAGCTCCTTATTATCGAAAGTTAAATTCTTCGAATGGTTAGCTGAAGTCTTTAAAAATAACCTGGCATTTGATGGTCACGGTAACGTTGCTTTCTTCGTTATTATTTTCCTCAGCATTATCGTGCGTTATTTCTTCGCTTCCGGTAGTGCCTATATCGTTGCTATGTTACCGGTATTTGCCATGCTGGCGAACGTCTCCGGCGCACCGTTAATGTTAACCGCGCTGGCACTGTTGTTCTCCAACTCCTATGGCGGCATGGTTACTCACTATGGCGGCGCGGCAGGTCCGGTCATCTTTGGC  >  NC_000913/802715‑803194

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑