Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	71,134	G→A	G250S (GGT→AGT)	araC →	DNA‑binding transcriptional dual regulator AraC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	71,134	0	G	A	100.0%	30.5 / NA	12	G250S (GGT→AGT)	araC	DNA‑binding transcriptional dual regulator AraC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (3/9);  total (3/9)

CCATCCACCGATGGATAATCGGGTACGCGAGGCTTGTCAGTACATCAGCGATCACCTGGCAGACAGCAATTTTGATATCGCCAGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGGTAACGAATCAGACAATTGACGGCTTGACGGAGTAGCATAGGGTTTGCAGAATCCCTGCTTCGTCCATTTGACAGG  >  NC_000913/70899‑71344                                                                                                                                                                                                                                            |                                                                                                                                                                                                                   ccatccaCCGATGGATAATCGGGTACGCGAGGCTTGTCAGTACATCAGCGATCACCTGGCAGACAGCAATTTTGATATCGCCAGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCAGTCGCAATGTTgn                                                                                                                                                                                                       <  1:56300/249‑2 (MQ=255)                       gTACGCGAGGCTTGTCAGTACATCAGCGATCACCGTGCAGACAGCAATTTTGATATCGCCAGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGTCTGTCACATCGTTTCCGCCAGCAGTTAGGGATTATCGTCTTAAGCTTGCTCGACGACCAACGCATTATTCATGCGAAGCGGCTTTTTAGCACTACCCGGTTGCCTAGCGCCACTATCAGTCGCAATGTTGGTTTTGACGATCAACTCTATTtctc                                                                                                                                                                               <  1:220009/251‑1 (MQ=255)                            cgAGGCTTGTCAGTACATCAGCGATCACCTGGCAGACAGCAATTTTGATATCGCCAGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCAGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAg                                                                                                                                                                          <  1:144279/251‑1 (MQ=255)                                                                          gcgatCGCCAGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACTAACGCATGAGTCAGGCGATGTTGCTTTTGAGCACTGCCTTGATGCCTATCGCCACCGTCAGTCGCTATTTTGGTTTTTACGATCATCTCTATCTCTCGCGAGTATTTATAAAATGCACCGGAGCCAGTCCGAGCGAGTTTCGTgtcg                                                                                                                             <  1:192670/247‑4 (MQ=255)                                                                               cGCCAGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGTCGGTCACATCTTTTCCGCCAGCAGTTATGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTGGAGCACTACCCGGATCCCTATCGCCTCCGTCAGTCTCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTtgt                                                                                                                        <  1:148173/250‑1 (MQ=255)                                                                                  cAGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCAGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGTTTGTgaag                                                                                                                    <  1:168923/251‑1 (MQ=255)                                                                                         gCACAGCATGTTTGCTTGTCGCCGTCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCAGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGt                                                                                                             >  1:71216/1‑251 (MQ=255)                                                                                         gCACAGCATGTTTGCTTGTCGACGTCGCGGCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCCGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCAGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTaaaaaa                                                                                                                                                               >  1:72004/1‑201 (MQ=255)                                                                                                                                       gCCAGCGGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTGGAGCCCTACCCGGATGCTTATCGCCACCGTCAGTCGCAATTTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCCCCGGGGCCAGCCCGAGCGATTTTCGTGCCGGTTGTGAAAAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGGTAACGAATCAGaaa                                                               <  1:202248/251‑3 (MQ=255)                                                                                                                                                          agTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCAGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGGTAACGAATCAGACAATTGACGGCTTGACGGAGt                                            >  1:18475/2‑251 (MQ=255)                                                                                                                                                                                                                cACTACCCGGATGCCTATCGCCACCGTCAGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGGTAACGAATCAGACAATTGACGGCTTGACGGAGTAGCATAGGGTTTGCAGAATCCCTGCTTCGTCCATTTGACAgn  <  1:25100/239‑2 (MQ=255)                                                                                                                                                                                                                 ncNACNCGGATNCCTANCGCCACCGTCAGTCGNAATGTTGGTTTTGACGATCNNCNCTATTNCTcgc                                                                                                                                                                             <  2:92575/66‑1 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                   CCATCCACCGATGGATAATCGGGTACGCGAGGCTTGTCAGTACATCAGCGATCACCTGGCAGACAGCAATTTTGATATCGCCAGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGGTAACGAATCAGACAATTGACGGCTTGACGGAGTAGCATAGGGTTTGCAGAATCCCTGCTTCGTCCATTTGACAGG  >  NC_000913/70899‑71344

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑