Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,337,623	G→A	Q103* (CAA→TAA)	adiY ←	DNA‑binding transcriptional activator AdiY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,337,623	0	G	A	100.0%	20.3 / NA	9	Q103* (CAA→TAA)	adiY	DNA‑binding transcriptional activator AdiY
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (5/4);  total (5/4)

AACTTTTTCTTTAACAGACTTGGGCTAAGACATAAACAACTGGCTACCATACTAAGATTCCAGTCTTTGTGAATATCGCTTTCAATAATTTGATAAACGCTGTCACTTACACAATTACGTAACATATACATCATTAATGAAACGAATTTTTTACTGTCGAGAAAACGGGATAGCACCGTGAATAATAATGCGCGTGTTCGTTCCTTTTCTGACTCAGTTTCTGCGGGCATCATGCTGTGTTGCGCGGCAACGCGAAAGACGTCTGGCGTCAGGCATGGCAGGGTGAGTATGGGCGTAGCGCTACGTTGCCATACTGGTATTTGCGAGAGATCTTTATTCAGAAACCGGAGGTAATCTTTGATGATGTCGTGACTAATATGAGCAACCAAAGTGTTGTTAAGAGAGGAGACGTCGATAATATTATTTTCACAATTTAATAACGCCATATGGTTAGCTTTAAG  >  NC_000913/4337382‑4337842                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                            aaCTTTTTCTTTAACAGACTTGGGCTAAGACATAAACAACTGGCTACCATACTAAGATTCCAGTCTTTGTGAATATCGCTTTCAATAATTTGATAACCGCTGTCACTTACACAATTACGTAACATATACATCATTAATGAAACGAATTTTTTACTGTCGAGAAAACGGGATAGCACCGTGAATAAGAATGCGCGTGTTCGTTCCTTTTCTGACTCAGTTTCTGCGGGCATCATGCTGTGTTACGCGGCAAc                                                                                                                                                                                                                    <  1:202792/251‑1 (MQ=255)                                                                                            naTAAACGCTGTCACTTACACAATTACGTAACATATACATCATTAATGAAACGAATTTTTTACTGTCGAGAAAACGGGATAGCACCGTGAATAATAATGCGCGTGTTCGTTCCTTTTCTGACTCAGTTTCTGCGGGCATCATGCTGTGTTACGCGGCAACGCGAAAGACGTCTGGCGTCAGGCATGGCAGGGTGAGTATGGGCGTAGCGCTACGTTGCCATACTGGTATTTGCGAGAGATCTTTATTc                                                                                                                            >  1:128596/2‑248 (MQ=255)                                                                                                                        tAACATATACATCATTAATGAAACGAATTTTTTACTGACGAGAAAACGGGATAGCACCGTGAATAATAATGCGCGTGTTCGTTCCTTTTCTGACTCAGTTGCGGCGGTCATCATGCTGTGTTACGCggn                                                                                                                                                                                                                       <  1:64760/129‑2 (MQ=255)                                                                                                                                aCATCATTAATGAAACGAATTTTTTACTGTCGAGAAAACGGGATAGCACCGTGAATAATAATGCGCGTGTTCGTTCCTTTTCTGACTCAGTTTCGGCGGGCATCAGGCTGTGTTACGCGGCAACGCGAAAGACGTCTGGCGTCAGGCATGGCAGGGTGAGTATGGGCGTAGCGCTACGTTGCCATACTGGTATTTGCGAGAGATc                                                                                                                                   <  1:83995/205‑1 (MQ=255)                                                                                                                                    natTAATGAAACGAATTTTTTACTGTCGAGAAAACGGGATAGCACCGTGAATACTAATGCGCGTGTTCGTTCCTTTTCTGACTCAGTTTCTGCGGGCATCATGCTGTGTTACGCGGCAACGCGAAAGACGTCTGGCGTCAGGCATGGCAGGGTGAGTATGGGCGTAGCGCTACGTTGCCATACTGGTATTTGCGAGAGATCTTTa                                                                                                                               >  1:55745/2‑205 (MQ=255)                                                                                                                                                 aaTTTTTTACTGTCGAGAAAACGGGATAGCGCCGTGAATAATAATGCGTGTGTTCGTTCCTTTTCTGACTCAGTTTCTGCGGGCATCATGCTGTGTTACGCGGCAACTCGAAAGACGTCTGGCGTCAGGCATGGCAGGGTGAGTATGGGCCTAGCGCTACTTTGCCATACTTGTATTTGCGAGAGATCTTTATTCAGAAACCGGAGGTAATCTTTGATGATGTCGTGACTAATATGAGCAACCAAAgtgt                                                                     <  1:94855/250‑1 (MQ=255)                                                                                                                                                           tGTCGAGAAAACGGGATAGCACCGTGAATAATAATGCGCGTGTTCGTTCCTTTTCTGACTCAGTTTCTGCGGGCATCATGCTGTGTTACGCGGCAACGCGAAAGACGTATGGCGTCAGGCATGGCAGGGAGAGNATGNNNNNAgnnnnnn                                                                                                                                                               >  2:137588/1‑144 (MQ=255)                                                                                                                                                                         gATAGCACCGTGAATAATAATGCGCGTGTTCGTTCCTTTTCTGACTCAGTTTCTGCGGGCATCATGCTGTGTTACGCGGCAACGCGAAAGACGTCTGGCGTCAGGCATGGCAGGGTGAGTATGGGCGTAGCGCNACgnnnnnctnnnnnn                                                                                                                                                 >  2:132945/1‑137 (MQ=255)                                                                                                                                                                                                                          tttCTGCGGGCATCATGCTGTGTTACGCGGCAACGCGACAGACGTCTGAGGTCCGGAGTGGAAGGGAGAGTATGGGCGAAGCGCTACGTTGCAATACTGGAACTTGCGAGAGCACTTTATTCAGCAACCGGAGGAAATCTTTGATGATGTCGTGACCAATATGAGCAACAAAAGAGTTGTTAAGAGAGGAGACGTCGATAATATTATTTTCACAATTTAATAACGCCATATGGTTAGCTTTAAg  >  1:105737/1‑244 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                            AACTTTTTCTTTAACAGACTTGGGCTAAGACATAAACAACTGGCTACCATACTAAGATTCCAGTCTTTGTGAATATCGCTTTCAATAATTTGATAAACGCTGTCACTTACACAATTACGTAACATATACATCATTAATGAAACGAATTTTTTACTGTCGAGAAAACGGGATAGCACCGTGAATAATAATGCGCGTGTTCGTTCCTTTTCTGACTCAGTTTCTGCGGGCATCATGCTGTGTTGCGCGGCAACGCGAAAGACGTCTGGCGTCAGGCATGGCAGGGTGAGTATGGGCGTAGCGCTACGTTGCCATACTGGTATTTGCGAGAGATCTTTATTCAGAAACCGGAGGTAATCTTTGATGATGTCGTGACTAATATGAGCAACCAAAGTGTTGTTAAGAGAGGAGACGTCGATAATATTATTTTCACAATTTAATAACGCCATATGGTTAGCTTTAAG  >  NC_000913/4337382‑4337842

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑