Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,272,338	G→A	L512L (CTG→TTG)	uvrA ←	UvrABC excision nuclease subunit A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,272,338	0	G	A	100.0%	10.4 / NA	7	L512L (CTG→TTG)	uvrA	UvrABC excision nuclease subunit A
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (4/3);  total (4/3)

GCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATTGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGATCGCGCAGATGGATAAGCGTACCCAACAGGCGCTCGTTATCACGCTGGTGCAGGCCGATAGACGGCTCGTCCAGCACGTACATAACGCCAACCAGGCCCGCACCAATCTGGCTCGCCAGACGGATACGCTGTGCTTCACCGCCAGAAAGCGTTTCTGCCGAGCGGGAAAGCGTCAGGTAATTCAGGCCGACGTTAACGAGGAATTTCAGACGATCGCCGATCTCTTTAAGGATTTTTTCTGCAATCTTCG  >  NC_000913/4272104‑4272514                                                                                                                                                                                                                                           |                                                                                                                                                                                 cccgggaccacacccccgcccccGCCATAATCGCTTCCAGCGGACCTTCGGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATTGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGATCGCGCAGATGGATAAGCGTACCCAACAGGCGCTCGTTAGCACGCTGGTGCAGGCCGATAGACGGCTCGTCCAACACGTACATAACGc                                                                                                                                                                    <  1:181980/228‑1 (MQ=255)                                                                                  ccGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATTGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGATCGCGCAGATGGATAAGCGTACCCAACAGGCGCTCGTTATCACGCTGGTGCAGGCCGATAGACGGCTCGTCCAACACGTACATAACGCCAACCa                                                                                                                                                              >  1:102208/1‑174 (MQ=255)                                                                                                                                         caccacAATCACGGTATTACCGAGATCGCGCAGATGGATAAGCGTACCCAACAGGCGCTCGTTATCACGNTGGNGCAGNCCNATAGACGGCTCGTCCNACACGTACATAACGCCAACCAGGCCCGCACCAATCTGGCTCGCCNNANNNANACGCtgtg                                                                                                                       <  2:148831/158‑1 (MQ=255)                                                                                                                                         caccacAATCACGGTATTACCGAGATCGCGCAGATGGATAAGCGTACCCAACAGGCGCTCGTTATCACGCTGGTGCAGGCCGCTAGACGGCTCGTCCAACACGTACATAACGCCAACCAGGCCCGCACCAATCTGGCTCGCCAGACGGATACGCtgtg                                                                                                                       >  1:148831/1‑158 (MQ=255)                                                                                                                                                        aTTACCGAGATCGCGCAGATGGATAAGCGTACCCAACAGGCGCTCGTTATCACGCAGCTGCAGGCCGCTAGACGGCTCGTCAAACCCGTACATAACGCCAACCAGGCCCGCACCAATATGGCTCGCCAGACGGCTACGCTGTGATTCACCGCCAGACAGCGTTTCTGCCGAGCGGGAAAGCg                                                                                >  1:116464/1‑182 (MQ=255)                                                                                                                                                              gagaTCGCGCAGATGGATAAGCGTACCCAACAGGCGCTCGTTATCACGCTGGTGCAGGTCGATAGACGGCTCGTCCAACACGTACATAACGCCAACCGGGCCCGCAc                                                                                                                                                     >  1:95558/1‑107 (MQ=255)                                                                                                                                                                  tCGCGCAGATGGATAAGCGTACCCAACAGGCGCTCGTTATCACGCTGGTGCAGGCCGATAGACGGCTCGTCCAACACGTACATAACGCCAACCAGGCCCGCACCAATCTGGCTCGCCAGACGGATACGCTGTGCTTCACCGCCAGAAAGCGTTTCTGCCGAGCGGGAAAGCGTCAGGTAATTCAGGCCGACGTTAACGAGGAATTTCAGACGATCGCCGATCTCTTTAAGGATTTTTTCTGCAATCTTCg  <  1:199181/250‑1 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                 GCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATTGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGATCGCGCAGATGGATAAGCGTACCCAACAGGCGCTCGTTATCACGCTGGTGCAGGCCGATAGACGGCTCGTCCAGCACGTACATAACGCCAACCAGGCCCGCACCAATCTGGCTCGCCAGACGGATACGCTGTGCTTCACCGCCAGAAAGCGTTTCTGCCGAGCGGGAAAGCGTCAGGTAATTCAGGCCGACGTTAACGAGGAATTTCAGACGATCGCCGATCTCTTTAAGGATTTTTTCTGCAATCTTCG  >  NC_000913/4272104‑4272514

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑