Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,268,466	G→A	intergenic (+159/+343)	tyrB → / ← yjbS	tyrosine aminotransferase/uncharacterized protein YjbS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,268,466	0	G	A	100.0%	30.5 / NA	13	intergenic (+159/+343)	tyrB/yjbS	tyrosine aminotransferase/uncharacterized protein YjbS
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (6/7);  total (6/7)

GCGGTGATGTAATGCAGGAAAGCAGGCTGGAGCTACCCAGCCTGCAGTGAAATTAAACTGTCGTCGCTTTCACTCTTTCTTTATAGATGATTTTTTTGATGCCATCGTTCTACGTGAGAGATAATAAACGTTGTTAGTTCTTTTATTGTTAAGCTTATCCCAATTATCTGGAATTCCTTATCCTGTTTTTTTGGGTGGAGTAATAATTTCTGCACATCTAACCATTATTAAATTTAATAATAAGTATCGGGTTTGTTACGATAGAATATTTCTATATCCTCAAGAATATGCTTTGCCTTATTGCCTGTGACTTTCTGAGATTCAATTATAGTATCGTTAAATTCTAATGTTAAAGAGAACTCTTTTTTCCGCTTTGTGT  >  NC_000913/4268296‑4268674                                                                                                                                                                           |                                                                                                                                                                                                                 gcGGTGATGTAATGCAGGAAAGCAGGCTGGAGCTACCCAGCCTGCAGTGAAATTAAACTGTCGTCGCTTTCACTCTTTCTTTATAGATGATTTTTTTGATGCCATCGTTCTACGTGAGAGATAATAAACGTTGTTAGTTCTTTTATTGTTAAGCTTATCCCAATTATCTGAAATTCCTTATCCTGTTTTTTTGGGTGGAGTAATAATTTCTGCACATCTAACCATTATTAAATTTAATAATAAGTATcn                                                                                                                                    <  1:42137/249‑2 (MQ=255)   ggTGATGTAATGCAGGAAAGCAGGCTGGAGCTACCCAGCCTGCAGTGAAATTAAACTGTCGTCGCTTTCACTCTTTCTTTATAGATGATTTTTTTGATGCCATCGTTCTACGTGAGAGATAATAAACGTTGTTAGTTCTTTTATTGTTAAGCTTATCCCAATTATCTGAAATTCCTTATCCTGTTTTTTTGGGTGGAGTAATAATTTCTGCACATCTAACCATTATTAAATTta                                                                                                                                                 >  1:15361/1‑234 (MQ=255)                                                 gAAATTAAACTGTCGTCGCTTTCACTCTTTCTTTATAGCTGATTTTTTTGATGCCATCGTTCTACGTGAGAGATAATAAACGTTGTTAGTTCTTTTATTGTTAAGCTCATCCCAATTATCTGAAATTCCTTATCCTGTTTTTTTGGGTGGAGTAATAATTTCTGCACATCTAACCATTATTAAATTTAATAATAAGTATCGGGTTTGTTACGATAGAATATTTCTATATCCTCAAGAATATGCTTTGCCtt                                                                                  >  1:109653/1‑251 (MQ=255)                                                  agcgtAAACTGTCGTCGCTTTCACTCTTTCTTTATAGATGATTTTTTTGATGCCATCGTTCTACGTGAGAGATAATAAACGTTGTTAGTTCTTTTATTGTTAAGCTTATCCCAATTATCTGAAATTCCTTATCCTGTTTTTTTGGGTGGAGTAATAATTTCTGCACATCTAACCAGTATTAAATTTAATAATAAGTATCGGGTTTGTTACGATAGAATATTTCTATATCCTCAAGAATATGCTTTGCCTTa                                                                                 <  1:88252/247‑1 (MQ=255)                                                         aCTGTCGTCGCTTTCACTCTTTCTTTATAGATGATTTTTTTGATGCCATCGTTCTACGTGAGAGATAATAAACGTTGTTAGTTCTTTTATTGTTAAGCTTATCCCAATTATCTGAAATTCCTTATCCTGTTTTTTTGGGTGGAGTAATAATTTCTGCACATCTAACCATTATTAAATTTAATAATAAGTATCGGGTTTGTTACGATAGAATATTTCTATAACCTCAAGAATATGCTTTGCCTTATTGCCtg                                                                          <  1:100179/251‑1 (MQ=255)                                                                                                     gCCATCGTTCTACGTGAGAGATAATAAACGTTGTTAGTTCTTTTATTGTTAAGCTTATCCCAATTATCTGAAATTCCTTATCCTGTTTTTTTGGGTGGAGTAATAATTTCTGCACATCTCACCATTATTAAATTTAATAATAAGTATCGGGTTTGTTACGATAg                                                                                                                     >  1:152139/1‑164 (MQ=255)                                                                                                     gCCATCGTTCTACGTGAGAGATAATAAACGTTGTTAGTTCTTTTATTGATAAGCTTATCCCAATTATCTGACATTACTTATCCTGTTTTTTTGGGTGGACTAATAATTTCTGCACATCTAACCATTATTAAATTTCATAATACCTATCGGGTTTGTTACGATAg                                                                                                                     >  1:152132/1‑164 (MQ=255)                                                                                                     gCCATCGTTCTACGTGAGAGATAATAAACGTTGTTAGTTCTTTTAGTGTTAAGCTTATCCCAATTATCTGAAATTNCGTNTCCTNTTNTTTTGGGTGGAGTAANAATTTCTGCACATCTAACCATTATTAAATTTAATAATAAGTATCNNGNNNGNTACGATAg                                                                                                                     <  2:152139/164‑1 (MQ=255)                                                                                                     gCCATCGTTCTACGTGAGAGATAAGAAACGTTGTTAGTTCTTTTATTGTTAAGCTTATCCCAATTATCTGAAAGTNCTTNTCCTNTTNTTTTGGGTGGAGTAANAATTTCTGCACATCTAACCATTATTAAATTTAATAATAAGTATCNNGNNNGNGACGATAg                                                                                                                     <  2:152132/164‑1 (MQ=255)                                                                                                      ccATCGTTCTACGTGAGAGATAATAAACGTTGTTAGTTCTTTTATTGTTAAGCTTCTCCCAATTATCTGAAATTCCTTATCCTGTTTTTTTGGGTGGAGTAATAATTTCTGCACATCTAACCATTATTAAATTTAATAATAAGTATCGGGTTTGTTACGATAGAATATTTCTATATCCTCAAGAATATGCTTTGCCTTATTGCCTGTGACTTTCTGAGATTCAATTATAGTATCGTTAAATTCTAATGtt                              >  1:147469/1‑250 (MQ=255)                                                                                                                        gaTAATAAACGTTGTTAGTTCTTTTATTGTTAAGCTTATCCCAATTATCTGAAATTCCTTATCCTGTTTTTTTGGGTGGAGTAATAATTTCTGCACATCTAACCATTATTAAATTTAATAATAAGTATCGGGTTTGTTACGATAGAATATTTCTATATCCTCAAGAATATGCTTTGCCTTATTGCCGGTGACTTTCTGAGAGTCAATTATAGTATCGTTAAATTCTAATGTTAAAGAGAACTCTTTTTTcc           <  1:147078/251‑1 (MQ=255)                                                                                                                                 ngttgttAGTTCTTTTATTGTTAAGCTTATCCCAATTATCTGAAATTCCTTATCCTGTTTTTTTGGGTGGAGTAATAATTTCTGCACATCTAACCATTATTAAATTTAATAATAAGTATCGGGTTTGTTACGATAGAATATTTCTATATCCTCAAGAATATGCTTTGCCTTATTGCCTGTGACTTTCTGAGCTTCAATTATAGTATCGTTAAATTCTAATGTTAAAGAGAACTCTTTTTTCCGCTTTgtgt  >  1:38403/2‑251 (MQ=255)                                                                                                                                                    gtaaaGCTTATCCCAATTATCTGAAATTCCTTATCCTGTTTTTGTGGGTGGAGTAATAATTTCTGCACATCTAAACATTATTAAATAGAATAATAAGTATCGGGTTTGGTACGATAGAATAGTTTTATATCCTGAAGAATATGCTTTGCCGTAGTGCCTGTAACTTTCAAAGATTCAATTATAGTATCGTGAAATTCTAAAGTTAAAGAGAACTCttt                <  1:114834/215‑1 (MQ=255)                                                                                                                                                                           |                                                                                                                                                                                                                 GCGGTGATGTAATGCAGGAAAGCAGGCTGGAGCTACCCAGCCTGCAGTGAAATTAAACTGTCGTCGCTTTCACTCTTTCTTTATAGATGATTTTTTTGATGCCATCGTTCTACGTGAGAGATAATAAACGTTGTTAGTTCTTTTATTGTTAAGCTTATCCCAATTATCTGGAATTCCTTATCCTGTTTTTTTGGGTGGAGTAATAATTTCTGCACATCTAACCATTATTAAATTTAATAATAAGTATCGGGTTTGTTACGATAGAATATTTCTATATCCTCAAGAATATGCTTTGCCTTATTGCCTGTGACTTTCTGAGATTCAATTATAGTATCGTTAAATTCTAATGTTAAAGAGAACTCTTTTTTCCGCTTTGTGT  >  NC_000913/4268296‑4268674

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑