Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,258,208	G→A	S156N (AGC→AAC)	dinF →	DNA damage‑inducible protein F

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,258,208	0	G	A	100.0%	14.4 / NA	7	S156N (AGC→AAC)	dinF	DNA damage‑inducible protein F
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (3/4);  total (3/4)

CCGGGCTGACTGCGCAGGCTTATGGTGCCAAAAATCCTCAGGCATTAGCCCGTACGCTGGTGCAACCGTTGCTGTTGGCGTTGGGGGCTGGGGCGTTAATTGCGCTGCTGCGTACGCCGATTATCGATCTGGCGCTGCATATTGTTGGCGGTAGTGAGGCAGTCCTGGAACAGGCGCGGCGCTTTCTTGAAATCCGCTGGTTAAGCGCACCGGCGTCGCTGGCGAATCTGGTATTACTCGGTTGGTTACTCGGCGTGCAATATGCCCGTGCGCCAGTAATTTTGTTAGTGGTCGGCAATATCCTCAACATTGTGCTGGATGTCTGGCTGGTGATGGGGCTGCATATGAACGTGCAGGGCGCGGCGCTGGCGACGGTTATTGCG  >  NC_000913/4258004‑4258386                                                                                                                                                                                                             |                                                                                                                                                                                   ccGGGCTGACTGCGCAGGCTTATGGTGCCAAAAATCCTCAGGCATTAGCCCGTACGCTGGTGCAACCGTTGCTGTTGGCGTTGGGGGCTGGGGCGTTAATTGCGCTGCTGCGTACGCCGATTATCGATCTGGCGCTGCATATTGTTGGCGGTAGTGAGGCAGTCCTGGAACAGGCGCGGCGCTTTCTTGAAATCCGCTGGTTAAACGCACCGGCGTCGCTGGCGAATCTGGTATTACTCGGTTGGTTACTc                                                                                                                                      <  1:35960/251‑1 (MQ=255)                                                              gCAACCGTTGCTGTTGGCGTTGGGGGCTGGGGCGTTAATTGCGCTGCTGCGTACGCCGATTATCGATCTGGCGCTGCATATTGTTGGCGGTAGTGAGGCAGTCCTGGAACAGGCGCGGCGCTTTCTTGAAATCCGCTGGTTAAACGCACCGGCGTCGCTGGCGAATCTGGTATTACTCGGTTGGTTACTCGGCGTGCAATATGCCCGTGCGCCAGTAATTTTGTTAGTGGTCGGCAATATCCTCAACAttn                                                                         <  1:129878/251‑2 (MQ=255)                                                                                gTTGGGGGCTGGGGCGTTAATTGCACTGCTGCGTACGCCGTTTATCGCCCTGGCGCTGCATATTGTTGGCGGTAGTGAGGCAGTCCTGGCACAGGCGCGGCGCTTTTTATAAATTCGCAGGTTAAACGCACCGGCGACGCTGGCGAATCTGGTATTACTCGGGTGGTTACTCGCCGTGCAATATGACCGTGCGCCAGTACTTTTGTTTGTGGGCGGCAATATCCTCCACATTGTGCTGGATGTctggctgg                                                       >  1:208044/1‑251 (MQ=255)                                                                                               gTTAATTGCGCTGCTGCGTACGCCGATTATCGATCTGGCGCTGCATATTGTTGGCGGTAGTGAGGCAGTCCTGGAACAGGCGCGGCGCTTTCTTGAAATCCGCTGGTTAAAAGCACCGGCGTCGCTGGCGAATCTGGTATTACTCGGTTGGTTACTCGGCGTGCAATATGCCCGTGCGCCAGTAATTTTGTTAGTGGTCGGCAATATCCTCAACATTGTGCTGGATGTCTGGCTGGTGATGGGGCTGCat                                         >  1:7258/1‑250 (MQ=255)                                                                                                           gctgcGTACGCCGATTATCGATCTGGCGCTGCATATTGTTGGCGGTAGTGAGGCAGTCCTGGAACAGGCGCGGCGCTTGCTTGAAATCCGCTGGTTAAACGCACCGGCGTCGCTGGCGAATCTGGTATTACTCGGTTGGTTACTCgg                                                                                                                                    <  1:35295/147‑1 (MQ=255)                                                                                                                                     cgcTGCATATTGTTTGCGGTAGTGAGGCAGTCCTGGAACAGGCGCGGCGCTTTCTTGAAATCCGCTGGTTAAACGCACCGGCGTCGCTGGCGAATCTGGTATTACTCGGTTGGTTACTCGGCGTGCAATATGCCCGTGCGCCAGTAATTTTGTTAGTGGTCGGCAATATCCTCAACATTGTGCTGGATGTCTGGCTGGTGATTGGGCTGCATATGAACGTGCAGGGCGCGGCGCTGGCGACGGTTATTGCg  <  1:153785/251‑1 (MQ=255)                                                                                                                                                                                             gAAATCCGCTGGTTAAACGCACCGGCGTCGCTGGCGAATCTGGTATTACTCGGTTGGTTACTCGGCGTGCAATATGCCCGTGCGCCAGTAATTTTGTTAGTGGTCGGCAATATCCTCAACATTGTGCTGGATGTCTGGCTGGTGATGGGGc                                              >  1:6086/1‑151 (MQ=255)                                                                                                                                                                                                             |                                                                                                                                                                                   CCGGGCTGACTGCGCAGGCTTATGGTGCCAAAAATCCTCAGGCATTAGCCCGTACGCTGGTGCAACCGTTGCTGTTGGCGTTGGGGGCTGGGGCGTTAATTGCGCTGCTGCGTACGCCGATTATCGATCTGGCGCTGCATATTGTTGGCGGTAGTGAGGCAGTCCTGGAACAGGCGCGGCGCTTTCTTGAAATCCGCTGGTTAAGCGCACCGGCGTCGCTGGCGAATCTGGTATTACTCGGTTGGTTACTCGGCGTGCAATATGCCCGTGCGCCAGTAATTTTGTTAGTGGTCGGCAATATCCTCAACATTGTGCTGGATGTCTGGCTGGTGATGGGGCTGCATATGAACGTGCAGGGCGCGGCGCTGGCGACGGTTATTGCG  >  NC_000913/4258004‑4258386

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑