Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,220,852	G→A	L546F (CTT→TTT)	arpA ←	regulator of acetyl CoA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,220,852	0	G	A	100.0%	27.5 / NA	12	L546F (CTT→TTT)	arpA	regulator of acetyl CoA synthetase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (5/7);  total (5/7)

TAAACGTATTAAACAGAGTGGGTAATGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTCCTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACAAAGGTAACGCGTTCAAAACAATTTTCAGCATGTCGGCATCACCATTCGCCATAACATGGTATAAAACATGGCTGGACGTTCTATTTTTGGCACTCAGGAATTTATACACTTGTTCGT  >  NC_000913/4220675‑4221097                                                                                                                                                                                  |                                                                                                                                                                                                                                                      tAAACGTATTAAACAGAGTGGGTAATGCTTTAAAAATAGTATTAATATCGTTCATGTGGCCGCGCTTCATGGCCATAATCAAACCCGTTTCGCGCGCAAGACTGTTATCGGTCAGAAGAACTACAAGATCAGAAGCTGAAATGTGAAGGTTCTGGGTTATGCTGGGCTATGCATGTAAAATATCTTTCACGATATCTCGGTGTCCCTTGTGCATCGCCAGATGTAGTCCTGGTCTACCATAAAAATCCtt                                                                                                                                                                               <  1:157791/250‑1 (MQ=255)                                       gTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGACGATCTACAATATCGGAAGCTGAAATGTTAATTTCCTGGGCTAGGCTGGGCACTGCTTCAAAAATAACTTCCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATg                                                                                                                                        >  1:84049/1‑251 (MQ=255)                                                             cgcgCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGAGGTCAGAAGATATACAATATCGGAAGATGAAATGTTAAATTCATGGGCTAGGCTGGGCAATGCTTAAAAAATAAATTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCttt                                                                                                                                                 >  1:138853/1‑220 (MQ=255)                                                                                                 cAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTCCTGGGCTAGGCTGGGCAATGCTTCAAAAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACAAAGGTAACGCGTTCAAAACAATTTTCAGCATGTCGGCATc                                                                               >  1:7833/1‑250 (MQ=255)                                                                                                  aaGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTCCTGGGCTAGGCTGGGCAATGCTTCAAAAATAACTTTCACGATATCGCTATGTTCATTTTGCATCGCCAGATATAGTCCTGGGCAACCTTAAAAATCCTTTGCCTTCAGGAGAGCGATTACCTGTTCTTCAGTCAAAGGACAGGTGCGAATTAACAAAGGTAACGCGTTCAAAACAATTTTCAGCATGTCGGCATCAc                                                                             <  1:223434/251‑1 (MQ=255)                                                                                                            gCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGGTAATTTCCTGGGCTAGGCTGGGCAATGCTTCAAAAATAACTTTCACGATAGCGCGATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTg                                                                                                                                                                             <  1:99942/145‑1 (MQ=255)                                                                                                                        tCTACAATATCGGAAGCTGAAATGTTAATTTCCTGGGCTAGGCTGGGCAATGCTTCAAAAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCaan                                                                                                                                                                                            <  1:64505/118‑2 (MQ=255)                                                                                                                                                   aTTTCCTGGGCTAGGCTGGGCAATGCTTCAAAAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACAAAGGTAACGCGTTCAAAACAATTTTCAGCATGTCGGCATCACCATTCGCCATAACATGGTATAAAACATGGCTGGACGTTCTATTTTTGGc                            <  1:187818/251‑1 (MQ=255)                                                                                                                                                                       cAATGCTTCAAAAATAACTTTCACGATCTCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGCTCGAGTCCCTGTTCTTTAGTCAAATGACCTGTGCGAATTAACAAAGGTAACGCGTTCAAAACAATTTTCAGCATGTCGGCATCACCATTCGCCATAACATGGTATAAAACATGGCTGGACGTTCTATTTTTGGCACTCAGGAATTTATACACtt        >  1:65919/1‑251 (MQ=255)                                                                                                                                                                            cTTCAAAAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCCACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACCTGTGCGAATTAACAAAGGAAACGCGTTCAAAACAATTTTCAGCATGTCGGCATCACCATTCGCCATAACATGGTATAAAACATGGCTGGACGTTCTATTTTTGGCACTCAGGAATTTATACACTTGTTCg   >  1:113172/1‑251 (MQ=255)                                                                                                                                                                            cTTCAAAAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACAAAGGTAACGCGTTCAAAACAATTTTCAGCATGTCGGCATCACCATTCGCCATAACATGGTATAAAACATGGCTGGACGTTCTATTTTTGGCACTCAgg                     <  1:1927/233‑1 (MQ=255)                                                                                                                                                                              tCAAAAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCATGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACAAAGGTAACGCGTTCAAAACAATGTGCATCAGGTCGGCATCACGATTCGCCATAACAGGGTGTAAAACATGGCTGGACGTGCTATTTTTGGCACTCAGGAATTTATACACTTGTTCGt  <  1:96975/250‑1 (MQ=255)                                                                                                                                                                                  |                                                                                                                                                                                                                                                      TAAACGTATTAAACAGAGTGGGTAATGCGTTAAAAATAGTATTAATAACGTTCATGTGTCCGCGCTGCATGGCCATAAACAAACCCGTGTCGCGCGCAAGACTTTTAGCGGTCAGAAGATCTACAATATCGGAAGCTGAAATGTTAATTTCCTGGGCTAGGCTGGGCAATGCTTCAAGAATAACTTTCACGATATCGCTATGTCCATTTTGCATCGCCAGATATAGTCCTGGGCAACCATAAAAATCCTTTGCCTTCAGGAGATCGAGTACCTGTTCTTTAGTCAAATGACATGTGCGAATTAACAAAGGTAACGCGTTCAAAACAATTTTCAGCATGTCGGCATCACCATTCGCCATAACATGGTATAAAACATGGCTGGACGTTCTATTTTTGGCACTCAGGAATTTATACACTTGTTCGT  >  NC_000913/4220675‑4221097

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑