Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,175,855	C→T	intergenic (+26/‑89)	thrT → / → tufB	tRNA‑Thr/translation elongation factor Tu 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,175,855	0	C	T	100.0%	21.6 / NA	9	intergenic (+26/‑89)	thrT/tufB	tRNA‑Thr/translation elongation factor Tu 2
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (5/4);  total (5/4)

GTATAATGGCTATTACCTCAGCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTCGGTACTATCGGCCACGTTGACCATGGTAAAACAACGCTGACCGCTGCAATCACTACCGTACTGGCTAAAACCTACGGCGGTGC  >  NC_000913/4175682‑4176071                                                                                                                                                                              |                                                                                                                                                                                                                         gTATAATGGCTATTACCTCAGCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCTTTCTGGTTATGGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTag                                                                                                                                             <  1:188020/251‑1 (MQ=255)                     gCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGAGAGAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGGGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCGTTGCTCCTCCCTGTTGTTTCTTTCTGTTTATTGCATTCAACAAGTCGGGCAt                                                                                                                                                                                           <  1:115844/185‑1 (MQ=38)                                                          cccGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCTTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTT‑GAACGTACAAAACCGCACGTTAACGTCgg                                                                                   >  1:159483/1‑251 (MQ=255)                                                                ncAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCATTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCTTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTCGGTACt                                                                               >  1:23372/2‑250 (MQ=255)                                                                         gCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCTTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTc                                                                                                                                                 >  1:70482/1‑175 (MQ=255)                                                                                                   cACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCTTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTCGGTACTATCGGCCACGTTGACCATGGTAAAACAACGCTGAcc                                           >  1:71521/1‑251 (MQ=255)                                                                                                               gggTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCTTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGGTGATGTGGTTATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTg                                                                                                               <  1:203760/171‑1 (MQ=255)                                                                                                                 ntGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCTTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGCTGTCTAAAGAAAAGTTTGAACGTACAACACAGCACGTTAACGTCGGTACTATCGGACACGTTGACCATGGTCAAACAACGCTGACCGCTGCAATCACTAc                             >  1:44322/2‑251 (MQ=255)                                                                                                                                             aagaaccacTTCTTTTCTCCTCCCTGTTTTTTCTTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTCGGTACTATCGGCCACGTTGACCATGGTAAAACAACGCTGACCGCTGCAATCACTACCGTACTGGCTAAAACCTACGGCGGtnc  <  1:170129/246‑1 (MQ=255)                                                                                                                                                                              |                                                                                                                                                                                                                         GTATAATGGCTATTACCTCAGCCTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTTGCCTGGTTGATGTGGTGATATCACCGATTTATCCGTGTCTTAGAGGGACAATCGATGTCTAAAGAAAAGTTTGAACGTACAAAACCGCACGTTAACGTCGGTACTATCGGCCACGTTGACCATGGTAAAACAACGCTGACCGCTGCAATCACTACCGTACTGGCTAAAACCTACGGCGGTGC  >  NC_000913/4175682‑4176071

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑