Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,118,837	C→T	intergenic (+77/+8)	zapB → / ← rraA	cell division factor ZapB/ribonuclease E inhibitor protein A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,118,837	0	C	T	100.0%	25.6 / NA	10	intergenic (+77/+8)	zapB/rraA	cell division factor ZapB/ribonuclease E inhibitor protein A
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (6/4);  total (6/4)

ACTGTCGCAGGAAGTTCAAAATGCCCAGCATCAGCGCGAAGAGCTGGAGCGTGAGAACAACCATCTGAAAGAACAGCAGAACGGCTGGCAGGAACGTCTGCAGGCC‑CTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGACACCGCCAAAATTGACGCGGACATCGCTTTCGCCAATGCCTTCGCCAGCGGCACCAACCGGAATTGCCGCCATCGCCTGGATGCCGATATCCAACTCTTCCAGGTCATCTACCTGACGCACCGC  >  NC_000913/4118628‑4119048                                                                                                                                                                                                                   |                                                                                                                                                                                                                    ncannnncnnnnnnnncannncgCCCANCNNCAGCGNGAANAGCTGGAGCGTGAGCACAACCATCTGAAAGAACAGCAGAACGGCTAGCAGGAACGTCTGCAGGCC‑CTGCTGTGTCGCATGGAAGAGGTCTGATTCCGCTACATCTNCCGNGTAANTTNTTTACTGAGTAGGCCNACAGGTAACGCATAAAAAAGGCACCTTGCGGTGCTGTTCTTATGNNTNNNTNGCCAGCgg                                                                                                                                                                                            <  2:153788/213‑1 (MQ=255)            aaGTTCAAAATGCCCAGCATCAGCGCGAAGAGCTGGAGCGCGAGAACAAACATCTGAAAGAACAGCAGAACGGCTGGCCGGACCGTCTGC‑GGCAACTGCTGGGTCGCATGGAAGAGGTCTGATTACGATTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGACACCTTGCGGTGCTTTTCTTATCATTCACTATCCAGCGGATCTTCTGAAAGAATAATCCCGGTAtt                                                                                                                                                                 >  1:208046/1‑251 (MQ=255)                               tCAGCGCGAAGAGCTGGCGCATGAGAACAACTATCAGAACGAACAGCAGAACGGCTGCAAGGAAAGTCAGCACGCC‑CTGCAGGGTCGCATGGACGACGACTGATCCCGATTCACCTTCCGCGTAATTTCTTTACTGTGTGGGCCAACAGGTAACGCAGAAAAAAGGCGACTTGCGGCACTTTTCTTAAAATTCCACATCCAGCGGATCTTCTGCAACCATAATCCAGGTAttcacagcacacaaatggacg                                                                                                                                              >  1:147359/1‑232 (MQ=255)                                                                               naaCGGCTGGCAGGAACGTCTGCAGGCC‑CTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCTTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGACACCGCCAAAATTGACGCGGACATCGCTTTCg                                                                                              >  1:23766/2‑251 (MQ=255)                                                                                                                                ngTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCTTTTCTTATCATTCCATATCCAGCGGATCTTCTGAAAGAATAATACCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGACACCGCCAAAATTGACGCGGACATCGCTTTCGCCAATGCCTTCGCCa                                                                               >  1:22306/2‑218 (MQ=255)                                                                                                                                           gCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCCGAAAACAGGCACCTTGCGGTGCTTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCCTAAAGATGGTCGCCGGAGAAGAAGGTGACACCGCCCAAATTGACGCGGACATCGCTTTCGCCAATGCCTTCGCCAGCGGCACCAACCGGAATTgccgc                                                        >  1:84140/1‑230 (MQ=255)                                                                                                                                              tCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCTTTTCTTATCATTCAAGATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAATAAGGTGACACCGCCAAAATTGACGCGGACATCGCGTTCGCCAATGCCTTCGCCAGCGGCACCAACCGGAATTGCCGCCATCGCCTGGATGCCGATATcc                                  <  1:219082/249‑1 (MQ=255)                                                                                                                                                 tCTTCCGCGTAATTTCTTTACTGTGTNGGCCAANNGGNAACGCAGAAAAAAGGCACCNTGNNNTNNNTNTCTTANCANNCAATATCCANCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGNGAAGAAGGTNNCACCGCCAAAATTGACGCGGANATCNNTNNCNCCANTg                                                                                        >  2:49768/1‑192 (MQ=255)                                                                                                                                                                     cTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCTTTTCTTATCATTCAATATCCACCGGATCTTCTGCCAGAATACTCCCGGTATTGTCGGCCTAAAGATGGTCGCCGGAGAAGAAGGTGACACCGCCAAAATTGACGCGGACATCGCTTTCGCCAATGCCTTCGCCAGCGGCACCAACCGGAATTGCCGCCATCGCCTGGATGCCGATATCCAACTCTTCCAGCTCATCTACCTGAc         >  1:119763/1‑251 (MQ=255)                                                                                                                                                                            ggCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCTTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGACACCGCCAAAAGGGACGCGGACATCGCGTTCGCCAATGTCTTCGGCAGCGTCACCAACCGTAAGTGCCGCCATCGCCTGGATGCCGATATCCAACTCTTCCAGGTCATCTACCTGACGCACcgc  <  1:217073/251‑1 (MQ=255)                                                                                                                                                                                            aGAAAAAAGGCACCTTGCGGTGCTTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCAGAAAGATGGTCGCCGGAGAATAAGGTGACACCGCCAAAATTGACGCGGACATCGCTTTCGCCAATGCCTTCGcc                                                                                <  1:146752/157‑1 (MQ=255)                                                                                                                                                                                                                   |                                                                                                                                                                                                                    ACTGTCGCAGGAAGTTCAAAATGCCCAGCATCAGCGCGAAGAGCTGGAGCGTGAGAACAACCATCTGAAAGAACAGCAGAACGGCTGGCAGGAACGTCTGCAGGCC‑CTGCTGGGTCGCATGGAAGAGGTCTGATTACGCTTCATCTTCCGCGTAATTTCTTTACTGTGTAGGCCAACAGGTAACGCAGAAAAAAGGCACCTTGCGGTGCCTTTCTTATCATTCAATATCCAGCGGATCTTCTGAAAGAATAATCCCGGTATTGTCGGCATAAAGATGGTCGCCGGAGAAGAAGGTGACACCGCCAAAATTGACGCGGACATCGCTTTCGCCAATGCCTTCGCCAGCGGCACCAACCGGAATTGCCGCCATCGCCTGGATGCCGATATCCAACTCTTCCAGGTCATCTACCTGACGCACCGC  >  NC_000913/4118628‑4119048

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑