Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,116,531	C→T	G231D (GGC→GAC)	glpK ←	glycerol kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,116,531	0	C	T	100.0%	22.4 / NA	9	G231D (GGC→GAC)	glpK	glycerol kinase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (5/4);  total (5/4)

CGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAAC  >  NC_000913/4116367‑4116737                                                                                                                                                                     |                                                                                                                                                                                                               cGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGAGTGGAATACGCGTGCCGCCTGTGCCGTCAATGTTAGTCTGACCGTATACTTCTTAAGAACGACGCACTTCTGACAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTg                                                                                                                          <  1:126901/251‑1 (MQ=255)                                          aTAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCCGCCGGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGTCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGCCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACg                                                                                 <  1:187330/251‑1 (MQ=255)                                                                        gCCATCACTTCTTTCACGAACAACTGACCAAACAGAGCGGCCTGCTGGTCCCCGGCGATGCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGTCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGGTGGTGTAATCGGTCACATGGac                                                   >  1:174967/1‑251 (MQ=255)                                                                                                     aaaCAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGTCAATGTTAGTCTGACCGTATACTTCGga                                                                                                                                                                                    <  1:83739/93‑1 (MQ=255)                                                                                                                      ngTNACCGGNGATCNCGGAGATTGGAATACNCGTGCCGCCTTTGCCGTCANNGNTAGTCNGACCg                                                                                                                                                                                               <  2:80238/64‑1 (MQ=255)                                                                                                                      ggTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGTCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTAcc      >  1:48147/1‑250 (MQ=255)                                                                                                                      ggTCACCGGCGATCCCGGAGAATGGAATACGCGTGCCGCCTTTGCCGTCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACGTCTGGCAGCATCTCGCGCGGACTATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGGTGGTGTAATCGGTGACATGGACACGGCCCTGCGTCATTTTCCAGATAAACCACGTATAAACCGTAcc      >  1:7837/1‑250 (MQ=255)                                                                                                                           ncGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGTCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAAc  >  1:26477/2‑249 (MQ=255)                                                                                                                                                   ngcgTGCCGCCTTTGCCGTCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCAtctc                                                                                                                                                         >  1:39060/2‑74 (MQ=255)                                                                                                                                                                     |                                                                                                                                                                                                               CGTTTTCTGATTTCACCGCTTTCTCGCCAGTGTTCATCAGCATAAAGCAGCCAGTGCCATAGGTGTTCTTCGCCATCCCTTCTTTCACGCACAACTGACCAAACAGCGCGGCCTGCTGGTCACCGGCGATCCCGGAGATTGGAATACGCGTGCCGCCTTTGCCGCCAATGTTAGTCTGACCGTATACTTCGGAAGAACGACGCACTTCTGGCAGCATCTCGCGCGGAATATCCAGCACTTCCAGCATTTTGTCGTCCCAGTCCAGGGTATGGATGTTGAACAACATGGTACGAGAGGCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAAC  >  NC_000913/4116367‑4116737

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑