Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,068,441	C→T	G279D (GGC→GAC)	yihQ ←	sulfoquinovosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,068,441	0	C	T	100.0%	23.2 / NA	11	G279D (GGC→GAC)	yihQ	sulfoquinovosidase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (2/9);  total (2/9)

CGCCAGAGGCATCTTTTGCCAGATAGCCGTGTTGTGCCGCTTCTTCGCAGAGATCTTTATCGCTGGCAACATACGGGTTGATATAGGCCAGGAACTGCACGCCCTCCTGATTCCACTGCTTAATGCGTGAATCCAGTTGCGGGTAGTTTTCGCTGTTCCACTTCCAGTTCCACATCACGCGTTTGCCAAAAGAGGTCATACGAATACCGGACCAGTCCTGCGCCCAGATGCCGTTGACCTTCACGCCCGCGTTACGCATGGTGTCCAGTTTCTTCTGGCACACTTCCGTCCCGCCCTGAATGCCGAGCGTTACTCCGTCATAAATCCAGTCGGGCAGTTCTGGCTGGCGTCCCAGCAGGGCGGTTAATTTTTCCAGCAGGGAAATGTATGTGTCAGCACATTCAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGTATTCCGGGG  >  NC_000913/4068196‑4068651                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                   cGCCAGAGGCATCTTTTGCCAGATAGCCGTGTTGTGCCGCTTCTTCGCAGAGATCTTTATCGCTGGCAACATACGGGTTGCTATAGGCCAGGAACTGCACGCCCTCCTGATTACACTGCTTAATGCGTGAATCCAGTTGCGGGTAGTTTTCGCTGTTCCACTTCCAGTTCCACATCACGCGTTTGCCAAAAGAGGTCATACGAATACCGGACCAGACCTGCGCCCAGATGCCGTTGACCTTCACGTCcgcg                                                                                                                                                                                                               >  1:152751/1‑251 (MQ=255)                            cGTGTTGTGCCGCTTCTTCGCAGAGATCTTTATCGCTGGCAACATACGGGTTGATATAGGCCAGGAACTGCACGCCCTCCTGATTCCACTGCTTAATGCGTGAATCCAGTTGCGGGTAGTTTTCGCTGTTCCACTTCCAGTTCCACATCACGCGTTTGCCAAAAGAGGTCATACGAATACCGGACCAGTCCTGCGCCCAGATGCCGTTGTCCTTCACGTCCGCGTTACGCATGGTGTCCAGTTTCTTCTgg                                                                                                                                                                                    <  1:197530/251‑1 (MQ=255)                             gtgtTGTGCCGCTTCTTCGCAGAGATCTTTATCGCTGGCAACATACGGGTTGATATAGGCCAGGAACTGCACGCCCTCCTGATTCCACTGCTTAATGCGTGAGTCCAGTTGCGGGTAGTTTTCGCTGTTCCACTTCCAGTTCCACATCACGCGTTTGCCAAAAGAGGTCATACGAATACCGGACCAGTCCTGCGCCCAGATGCCGTTGACCTTCACGTCCGCGTTACGCATGGTGTCCAGTTTCTTCTGGc                                                                                                                                                                                   <  1:213492/251‑1 (MQ=255)                                  gtgCCGCTTCTTCGCAGAGATCTTTATCGCTGGCAACATACGGGTTGATATAGGCCAGTAACTGCACGCCCTCCTGATTCCACTGCTTAATGCGTGAATCCAGTTGCTGGTAGTTTTCGCTGTTCCACTTCCAGCTCCACATCACGCGTTTGCCAAAAGAGGTCATACGAATACCGGACCAGTCCTGCGCCCAGATGCCGTTGACCTTCACGTCCGCGTTACGCATGGTGTCCAGTTTCTTCTGGCacac                                                                                                                                                                               <  1:28255/250‑1 (MQ=255)                                                                                        ccAGGAACTGCACGCCCTCCTGATTCCACTGCTTAATGCGTGAATCCAGTTGCGGGTAGTTTTCGCTGTTCCACTTCCAGTTCCACATCACGCGTTTGCCAAAAGAGGTCATACGAATACCGGACCAGTCCGGCGCCCAGATGCCGGTGACCGTCACGTCCGCGTTACGCATGGTGTCCAGTTTCTTCTGGCACACTTCCGTCCCGccc                                                                                                                                                                  <  1:109986/209‑1 (MQ=255)                                                                                                 gCACGCCCTCCTGATTCCACTGCTTAATGCGTGAATCCAGTTGCGGGTAGTTTTCGCTGTTCCACTTCCAGTTCCACATCACGCGTTTGCCAAAAGAGGTCATACGAATACCGGACCAGTCCTGCGCCCAGATGCCGTTGACCTTCACGTCCGCGTTACGc                                                                                                                                                                                                         <  1:187882/161‑1 (MQ=255)                                                                                                                                            cGGGTAGTTTTCGCTGTTCCACTTCCAGTTCCACATCACGCGTTTGCCAAAAGAGGTCATACGAATACCGGACCAGTCCTGCGCCCAGATGCCGTTGACCTTCACGTCCGCGTTACGCATGGTGTCCAGTTTCTTCTGGCACACTTCCGTCCCGCCCTGAATGCCGAGCGTTACTCCGTCATAAATCCAGTCGGGCAGTTCTTGCTGGCGTCCCAGCAGGGCGGTTAATTTTTCCAGCAGGGAAatgtatg                                                                    <  1:99703/251‑1 (MQ=255)                                                                                                                                                               cACTTCCAGTTCCACATCACGCGTTTGCCAAAAGAGGTCATACGAATACCTGACCAGTCCTGCGCCCAGATGCCGTTGACCTTCACGTCCGCGTTACGCATGGTGTCCAGTTTCTTCTGGCACACTTCCGTCCCGCCCTGAATGCCGAGCGTTACTCCGTCATAAATCCAGTCGGGCAGTTCTGGCTGGCGTCCCAGCAGGGCGGTTAATTTTTcc                                                                                    <  1:67636/216‑1 (MQ=255)                                                                                                                                                                                        tGCCAAAAGAGGTCATACGAATACCGGACCAGTCCTGCGCCCAGATGCCGTTGACCTTCACGTCCGCGTTACGCATGGTGTCCAGTTTCTTCTGGCACACTTCCGTCCCGCCCTGAATGCCGAGCGTTACTCCGTCATAAATCCAGTCGGGCAGTTCTGGCGGGCGTCCCAGCAGGGCGGTTAATTTTTCCAGCAGGTAAAGGTATGTGTCAGCACATTCAAAACGCAGCGTTGCTTTGTCTTCCCACAg                         <  1:144242/250‑1 (MQ=255)                                                                                                                                                                                                              aCCGGACCAGTCCTGCGCCCAGATGCCGTTGACCTTCACGTGCGCGTTACGCATGGAGTCCAGTTTCTTCTGGCACACTTCCGTCCCGCACTGAATGCAGAGCGGTACTCCGTCATAAATACAGTCGGGCAGTTCTGGCTGGCGTCCCAGCAGGGCGGTTAATTTTTACAGCAGGGAAATGTATGTGTCAGCACATTCAAAACGAAGCGTTGCTTTGTCTTCCCACAGCGCAAGTTCATGGTATTCCgggg  >  1:157882/1‑251 (MQ=255)                                                                                                                                                                                                                cGGACCAGGCCTGCGCCCAGATGCCGTTGACCTTCACGTCCGCGTTACGCATGGTGTCCATTTTCTTCGGGCACACTTCCGTCCCGCCCTGAATGCCGAGCGTTACTCCGTCATAAAGCCAGTCGGGCAGTTCTGGCTGGCGTCCCAGTAGGGCGGTTAATTTTTCCAGCAGGGAAATGTATGTGTCAGCACATTCAAAACg                                                 <  1:97835/202‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                   CGCCAGAGGCATCTTTTGCCAGATAGCCGTGTTGTGCCGCTTCTTCGCAGAGATCTTTATCGCTGGCAACATACGGGTTGATATAGGCCAGGAACTGCACGCCCTCCTGATTCCACTGCTTAATGCGTGAATCCAGTTGCGGGTAGTTTTCGCTGTTCCACTTCCAGTTCCACATCACGCGTTTGCCAAAAGAGGTCATACGAATACCGGACCAGTCCTGCGCCCAGATGCCGTTGACCTTCACGCCCGCGTTACGCATGGTGTCCAGTTTCTTCTGGCACACTTCCGTCCCGCCCTGAATGCCGAGCGTTACTCCGTCATAAATCCAGTCGGGCAGTTCTGGCTGGCGTCCCAGCAGGGCGGTTAATTTTTCCAGCAGGGAAATGTATGTGTCAGCACATTCAAAACGCAGCGTTGCTTTGTCTTCCCACAGCGCCAGTTCATGGTATTCCGGGG  >  NC_000913/4068196‑4068651

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑