Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,066,974 C→T G74D (GGC→GAC)  yihP ← putative 2,3‑dihydroxypropane‑1‑sulfonate export protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,066,9740CT88.9% 33.1 / ‑0.3 18G74D (GGC→GAC) yihPputative 2,3‑dihydroxypropane‑1‑sulfonate export protein
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base T (4/12);  total (5/13)
Fisher's exact test for biased strand distribution p-value = 4.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.56e-02

GCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGGTGTGCCGACAAAGTTGGCGATCGCCAGTAAGGTGACCGGGAATGACGCATACAGAATAAAAGGACGGAACTTACCTTTTGGACCGATCTTGCGTCGGGAATCCAACATAATGCCGGTACCCATATCGGTAAACGCAGTAAAGAATTTTGAAATCAAAAAGATAATGCCGCCATAGGTGCCTGGCAGCCCCAGAACGTCGGTATAAAACTTCAAAAGATAAAGCGTACCGATATCCAGCAGGATGTTAGAGGCCAGGTCGCCAATACCGTAAGAGAGTTTCTCTTTAAAGGGCAGGCGTAAAGTTGCTGGATCTTCCGTTGTGATGTGACTCATCGATGTTCTCCCTTTGCCCA  >  NC_000913/4066793‑4067214
                                                                                                                                                                                     |                                                                                                                                                                                                                                                
gCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGGGACAGCAAACGGTGTGCCGACAAAGTTGGCGATCGCCAGTAAGGTGACCGGGAATGACGCATACAGAATAAAAGGACGGAACTTACCTTTTGTACCGATCTTGCGTCGGGAAGCCAACATAATGTCGTTACCCATATCGGTAAACGCAGTAAAGAATTTTGAAATCAAAAAGATAATGCCGCCATAGGTGCCTg                                                                                                                                                                             <  1:209245/251‑1 (MQ=255)
   gAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGGTGTGCCGACAAAGTTGGCGATCGCCAGTAAGGTGACCGGGAATGACGCATACAGAATAAAAGGACGGAACTTACCTTTTGGACCGATCTTGCGTCGGGAATCCAACATAATGTCGGTACCCATAGCGGTAAACGCAGTAAAGAATTTTGAAATCAAAAAGATAATGCCGCCATAGGTGCCTGGc                                                                                                                                                                           <  1:220160/250‑1 (MQ=255)
      aaaCAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGGTGTGCCGACAAAGTTGGCGATCGCCAGTAAGGTGACCGGGAATGACGCATACAGAATAAAAGGACGGAACTTACCTTTTGGACCGATCTTGCGTCGGGAATCCAACATAATGTCGGTACCCATATCGGTAAACGCAGTAAAGAATTTTGAAATCAAAAAGATAAGGCCGCCATAGGTGCCTGGCAGcn                                                                                                                                                                       <  1:64511/251‑2 (MQ=255)
              cGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGGTGTGCCGACAAAGTTGGCGATCGCCAGTAAGGTGACCGGGAATGACGCATACAGAATAAAAGGACGGAACTTACCTTTTGGACCGATCTTGCGTCGGGAATCCAACATAATGTCGGTACCCATATCGGTAAACGCAGTAAAGAATTTTGAAATCAAAAAGATAATGCCGCCATAGGTGCCTGGCAGCCCCAGAAc                                                                                                                                                                >  1:69060/1‑250 (MQ=255)
                                  gTGGCCATCACCGTTTTACCGGTGACATCAAACGGTGTGCCGACAAAGTTGGCGATCGCCAGTAAGGTGACCGGGAAGGACGCATACAGAATAAAAGGACGGAACTTACCTTTTGGACCGATCTTGCGTCGGGAATCCAACATAATGTCGGTACCCATATCGGTAAACGCAGTAAAGAATTTTGAAATCAAAAAGATAATGCCGCCATAGGTGCCTGGc                                                                                                                                                                           <  1:68381/219‑1 (MQ=255)
                                                      ggTGACATCAAACGGTGTGCCGACAAAGTTGGCGATCGCCAGTAAGGTAACCGGGAATGACGCATACAGAATAAAAGGACGTAACTTACCTTTTGGACCGATCTTGCGTCGGGAATCCAACATAATGTCGGTACCCAGATCGGTAAGCGGAGTAAAGAATTTTGAAATCAAAAAGATAATGCCGCCATAGGTGCCTGGc                                                                                                                                                                           <  1:203507/199‑1 (MQ=255)
                                                                                     gCGATCGCCAGTAAGGTGACCGGGAATGACGCATACAGCATAAAAGGACGGAACTTACCTTTTGGACCGATCTTGCGTCGGGAATCCAACATAATGTCGGTACCCATATCGGTAAACGCAGTAAAGAATTTTGAAATCAAAAAGATAATGCCGCCATAGGTGCCTGGCAGCCCCAGAACGTCGGTATAAAACTTCAAAAGATAAAGCGTACCGATATCACGCAGGATGTTAGAGGCCAGGTCGCCAATAcc                                                                                        >  1:80711/1‑251 (MQ=255)
                                                                                     gCGATCGCCAGTAAGGTGACCGGGAATGACGCATACAGAATAAAAGGACGGAACTTACCTTTTGGACCGATCTTGCGTCGGGAATCCAACATAATGTCGGTACCCATATCGGTAAACGCAGTAAAGAATTTTGAAATCAAAAAGATAATGCCGCCATAGGTGCCTGGCAGCCCCAGAACGTCGGTATAAAACTTCAAAAGATAAAGCGTACCGATATCCAGCAGGAtn                                                                                                               <  1:64718/228‑2 (MQ=255)
                                                                                     gCGATCGCCAGTAAGGTGACCGGGAATGACGCATACAGAATAAAAGGACGGAACTTACCTTGTGGACCGATCTTGCGTCGGGAATCCAACATAATGCCGGTACCCATATCGGTAAACGCAGTAAAGAATTTTGAAATCAAAAAGAGAAGGCCGCCATAGTTGCCTGGCa                                                                                                                                                                          <  1:48691/169‑1 (MQ=255)
                                                                                     gCGATCGCCAGTAAGGTGACCGGGAANGACGCANNCANAATAAAAGGACGGAACTTANCTNNNGNNNCNCTCTTNCGNNGGGAATCCANCATAATGCCGGTACCCATATCGGTAAACGCAGTAAAGAATTTTGAAATCCAAANGATAATGCCNNCATAGGTGCCTGGCa                                                                                                                                                                          >  2:48691/1‑169 (MQ=255)
                                                                                      cGATCGCCCGTAAGGTGACCGGGAATGACGCATACAGAATAAAAGGACGTAACTTACCTTTTGGACCGATCTTGCGGCGGGAATCCAACATAATGTCGGTACCCATATCGGTAAACTCAGTAAAGAATTTTGAAATCAAAAAGATTATTCCGCCAGAGGTTCCTGGCAGCCCCAGAACGTCGGTATAAAACGTCAAAAGATAAAGCGTACCTATATCCAGCAGTATGTTAGAGGCCAGGTCGCCAATACCg                                                                                       <  1:164558/251‑1 (MQ=255)
                                                                                      cGATCGCCAGTAAGGTGACCGGGAATGACGCATACAGAATAAAAGGACGGAACTTACCTTTTGTACCGATCTTGCGTCGGGAATCCAACATAATGTCGGTACCCCTAGCGGTAAACGCAGTAAATAATTTTGAAACCAAAAAGATAATGCCTCCATAGGTGCCTGGCAGCCCCAGAACGTCTGTAGAAATCCTCAAAAGATAAAGCGTTCCGATATCCAGCAGGATGTTAGAGGCCAGGTCGCCAATCCCg                                                                                       <  1:164551/251‑1 (MQ=255)
                                                                                       gATCGCCAGTAAGGTGACCGGGAATGACGCATACAGAATAAAAGGACGGAACTTACCTTTTGGACCGATCTTGCGTCGGGAATCCAACATAATGTCGGTACCCATATCGGTAAACGCAGTAAAGAATTTTGAAATCAAAAAGATAATGCCGCCATAGGTGCCTGGCAGCCCCAGAACGTCGGTATAAAACTTCAAAAGATAAAGCGTACCGATATCCAGCAGGATGTTAGAGGCCAGGTCGCCAATACCGt                                                                                      >  1:49385/1‑251 (MQ=255)
                                                                                                      gACCGGGAATGACGCATACAGAATAAAAGGACGGAACTTACCTTTTGGACCGATCTTGCGTCGGGAATCCAACATAATGTCGGTACCCATATCGGTaa                                                                                                                                                                                                                                >  1:57686/1‑98 (MQ=255)
                                                                                                            gAATGACGTATACAGAATAAAAGGACGGAACTTACCTTTTGGACCGATCTTGCGTCGGGAATCCAACATAATGTCGGTACCCATATCGGTAAACGCAGTAAAGAATTTGGAAATCAAAAAGATAATGCCGCCATAGGTGCCTGGCAGCCCCAGAACGTCGGTATAAAACGTCAAAAGATAAAGCTTGCCGATAGCCAGCAGGATGTTAGAGGCCAGGTCGCCAATACCGTAAGAGAGTTTCTCTTTAAAgg                                                                 <  1:108072/251‑1 (MQ=255)
                                                                                                                              aaaaGGACGGAACTTACCTTTTGGACCGATCTTGCGTCGGGAATCCAACATAATGTCGGTACCCATATCGGTAAACGCAGTAAAGAATTTTGAAATCAAAAAGATAATGCCGCCATAGGTGCCTGGCAGCCCCAGAACGTCGGTATAAAACTTCAAAAGATAAAGCGTACCGATATCCAGCAGGATGTTAGAGGCCAGGTCGCCAATACCGTAAGAGAGTTTCTCTTTAAAGGGCAGGCGTAAAGTTGCTg                                               <  1:115266/251‑1 (MQ=255)
                                                                                                                                         aCTTACCTTTTGGACCGATCTTGCGTCGGGAATCCAACATAATGTCGGTACCCATATCGGTAAACGCAGTAAAGAATTTTGAAATCAAAAAGATAATGCCGCCATAGGTGCCTGGCAGCCCCAGAACGTCGGTATAAAACTTCAAAAGATAAAGCGTACCGATATCCAGCAGGATGTTAGAgg                                                                                                        <  1:166637/183‑1 (MQ=255)
                                                                                                                                                                           cAACATAATGTCGGTACCCATATCGGTAAACGCAGTAAAGAATTTTGAAATCAAAAAGATAATGCCGCCATAGGTGCCTGGCAGCCCCAGAACGTCGGTATAAAACTTCAAAAGATAAAGCGTACCGATATCCAGCAGGATGTTAGAGGCCAGGTCTCCAATACCGTAAGAGAGTTTCTCTTTAAAGGGCAGGCGTAAAGTTGCTGGATCTTCCGTTGTGATGTTACTCATCGATGTTCTCCCTTTGCCCa  <  1:156002/251‑1 (MQ=255)
                                                                                                                                                                                     |                                                                                                                                                                                                                                                
GCTGAAAAACAGTCCGTAAAGCATAAACAGAATAGTGGCCATCACCGTTTTACCGGTGACATCAAACGGTGTGCCGACAAAGTTGGCGATCGCCAGTAAGGTGACCGGGAATGACGCATACAGAATAAAAGGACGGAACTTACCTTTTGGACCGATCTTGCGTCGGGAATCCAACATAATGCCGGTACCCATATCGGTAAACGCAGTAAAGAATTTTGAAATCAAAAAGATAATGCCGCCATAGGTGCCTGGCAGCCCCAGAACGTCGGTATAAAACTTCAAAAGATAAAGCGTACCGATATCCAGCAGGATGTTAGAGGCCAGGTCGCCAATACCGTAAGAGAGTTTCTCTTTAAAGGGCAGGCGTAAAGTTGCTGGATCTTCCGTTGTGATGTGACTCATCGATGTTCTCCCTTTGCCCA  >  NC_000913/4066793‑4067214

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑