Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,066,466	C→T	P243P (CCG→CCA)	yihP ←	putative 2,3‑dihydroxypropane‑1‑sulfonate export protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,066,466	0	C	T	100.0%	16.3 / NA	8	P243P (CCG→CCA)	yihP	putative 2,3‑dihydroxypropane‑1‑sulfonate export protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (7/1);  total (7/1)

GCCAAAACGTCTGACTGAGGCAGGCATCAGGAATACGCCGATGAAAATACAGCCCATGCTGAAAAATCCCATATACGACAACAGGATGGGATCGTTAAGCACGTACTGGGTGTAATAGACCTGGATGGCGAGCTTGACGTTAAACGCCCCTAAAGTGCAGAGGTTGGCAATGCACAGAATGAACAGCGGGCGGTTACCCGCAATTGCGCGGAAAGATTGCAGCAGGCCCGGCTTTTGCGCCGGATTAGCAGGCTGGGTTTCGACATAACGCTCTTTCACGCCCGAGTAGCAGATCCACATAAACAGCAGGCCAAACAGTGAAAACAGCGTGGCGGCGAAGATATAGCCAAGTTGCTGATTACCTTCGATAAGATTCATAACTG  >  NC_000913/4066280‑4066662                                                                                                                                                                                           |                                                                                                                                                                                                     nccAAAACGTCTGACTGAGGCAGGCATCAGGAATACGCCGATGAAAATACAGCCCATGCTGAAAAATCCCATATACGACAACAGGATGGGATCGTTAAGCACGTACTGGGTGTAATAGACCTGGATGGCGAGCTTGACGTTAAACGCCCCTAAAGTGCAGAGGTTGGCAATGCACAGAATGAACAGTGGGCGGTTACCCGCAATTGCGCGGAAAGATTGCAGCAGGCCCGGCTTTTGCGCCGGATTAGc                                                                                                                                        >  1:54853/2‑249 (MQ=255)                            cAGGAATACGCCGATGAACATACAGCCCATGCTGAAAAATCCCATATACGACAACAGGATGGGATCGTTAAGCACGTACTGGGTGTAATAGACCTGGATGGCGAGCTTGACGTTAAACGCCCCTAAAGTGCAGAGGTTGGCAATGCACAGAATGAACAGTGGGCGGTTACCCGCAATTGCGCGGAAAGATTGCAGCAGGCCCGGCTTTTgcgc                                                                                                                                                 >  1:173064/1‑213 (MQ=255)                                                                                                         aCTGGGTGTAATAGACCTGGATGGCGAGCTTGACGTTAAACGCCCCTAAAGTGCAGAGGTTGGAAATGCACAGAATGAACAGTGGGCGGATACCCACACTTGAGCGGAAAGGTTGCAGCCGGCCCGGCTTTTGCGCCGGATTAGCAGGCTGGGGTTCGACATAACGCTCTTTCACGCCCGAGTAGCAGATCCACATAAACAGCAGGCCAAACAGAGAAAACAGCGCGGCGGCGAAGATATAGCCAAgatg                               >  1:157608/1‑247 (MQ=255)                                                                                                                  aaTAGACCTGGATGGCGAGCTTGACGTTAAACGCCCCTAAAGTGCAGAGGTTGGCAATGCACAGAATGAACAGTGGGCGGTTACCCGCAATTGCGCGGAAAGATTGCAGCAGGCCCGGCTTTTGCGCCGGATTa                                                                                                                                          >  1:3804/1‑134 (MQ=255)                                                                                                                          tGGATGGCGAGCTTGACGTTAAACGCCCCTAAAGTGCAGAGGTTGGCAATGCACAGAATGAACAGTGGGCGGTTACCCGCAATTGCGCNNAAAGATNnc                                                                                                                                                                     >  2:2181/1‑99 (MQ=255)                                                                                                                          tGGATGGCGAGCTTGACGTTAAACGCCCCTAAAGTGCAGAGGTTGGCAATGCACAGAATGAACAGTGGGCGGTTACCCGCAATTGCGCGGAAAGATTGCAGCAGGCCCGGCTTTTGCGCCGGATTAGCAGGCTGGGTTTCGGCATAACGCTCTTTCACGCCCGAGTAGCAGATCCACATAAACAGCAGGCCAAACAGTGAAAACAGCGTGGCGGCGAAGata                                          >  1:202953/1‑222 (MQ=255)                                                                                                                                     nttGACGTTAAACGCCCCTAAAGTGCAGAGGTTGGCAATGCACAGAATGAACAGTGGGCGGTTACCCGCAATTGCGCGGAAAGATTGCAGCAGGCCCGGCTTTTGCGCCGGATTAGCAGGCTGGGTTTCGACATAACGCTCTTTCACGCCCGAGTAGCAGATCCACATAAACAGCAGGCCAAACAGTGAAAACAGCGTGGCGGCGAAGATATAGCCAAGTTGCTGATTACCTTCGATAAGATTCATAACTg  >  1:40459/2‑251 (MQ=255)                                                                                                                                                               agagGTTGGCAATGCACAGAATGAACAGTGGGCGGTTACCCGCAATTGCGCGGAAAGATTGCAGCAGGCCCGGCTTTTGCGCCGGATTAGCAGGCTGGGTTTCGACATAACGCTCTTTCa                                                                                                           <  1:219573/120‑1 (MQ=255)                                                                                                                                                                                           |                                                                                                                                                                                                     GCCAAAACGTCTGACTGAGGCAGGCATCAGGAATACGCCGATGAAAATACAGCCCATGCTGAAAAATCCCATATACGACAACAGGATGGGATCGTTAAGCACGTACTGGGTGTAATAGACCTGGATGGCGAGCTTGACGTTAAACGCCCCTAAAGTGCAGAGGTTGGCAATGCACAGAATGAACAGCGGGCGGTTACCCGCAATTGCGCGGAAAGATTGCAGCAGGCCCGGCTTTTGCGCCGGATTAGCAGGCTGGGTTTCGACATAACGCTCTTTCACGCCCGAGTAGCAGATCCACATAAACAGCAGGCCAAACAGTGAAAACAGCGTGGCGGCGAAGATATAGCCAAGTTGCTGATTACCTTCGATAAGATTCATAACTG  >  NC_000913/4066280‑4066662

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑