Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,059,601	C→T	P399S (CCG→TCG)	bipA →	50S ribosomal subunit assembly factor BipA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,059,601	0	C	T	100.0%	18.6 / NA	9	P399S (CCG→TCG)	bipA	50S ribosomal subunit assembly factor BipA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (3/6);  total (3/6)

GGTCGTGGCGAACTGCACCTGTCTGTTCTGATCGAAAACATGCGTCGTGAAGGTTTCGAACTGGCGGTATCCCGTCCGAAAGTTATCTTCCGTGAAATCGACGGTCGTAAACAAGAGCCGTATGAAAACGTGACTCTGGACGTTGAAGAACAGCATCAGGGTTCTGTAATGCAGGCGCTGGGCGAACGTAAAGGCGACCTGAAAAACATGAATCCAGACGGTAAAGGCCGCGTACGTCTCGACTACGTGATCCCAAGCCGTGGTCTGATTGGCTTCCGTTCTGAGTTCATGAC  >  NC_000913/4059484‑4059776                                                                                                                      |                                                                                                                                                                                ggTCGTGGCGAACTGCACCTGTCTGTTCTGATCGAAAACATGCGTCGTGAAGGTTTCGAACTGGCGGTATCCCGTCCGAAAGTTATCTTCCGTGAAATCGACGGTCGTAAACAAGAGTCGTATGAAAACGTGANTCTNNNNNttnnnnnn                                                                                                                                                 >  2:135570/1‑144 (MQ=255) ggTCGTGGCGAACTGCACCTGTCTGTTCTGATCGAAAACATGCGTCGTGAAGGTTTCGAACTGGCGGTATCCCGTCCGAAAGTTATCTTCCGTGAAATCGACGGTCGTAAACAAGAGTCGTATGAAAACGTGACTCTGGACGTTGAAGAACAGCATCAGGGTTCTGTAATGCAGGCGCTGGGCGAACGTAAAGGCGan                                                                                                 <  1:135570/198‑2 (MQ=255)                         gtTCTGATCGAAAACATGCGTCGTGAAGGTTTCGAACTGGCGGTATCCCGTCCGAAAGTTATCTTCCGTGAAATCGACGGTCGTAAACAAGAGTCGTATGAAAACGTGACTCTGGACGTTGAAGAACAGCATCAGGGTTCGGTAATGCAGGCGCTGGGCGAACGTAAAGGCGACCTGAAAAACATTAATCCAGACGGGAAAGGCCGCGTACGTCTCGACTACGTGATCCCAAGCCGTGGTCTGATTgn                       <  1:38631/248‑2 (MQ=255)                           tCTGATCGAAAACATGCGTCGTGAAGGTTTCGAACTGGCGGTATCCCGTCCGAAAGTTATCTTCCGTGAAATCGACGGTCGTAAACAAGAGTCGTATGAAAACGTGACTCTGGACGTTGAAGAACAGCATCAGGGTTCTGTAATGCAGGCGCTGGGCGAACGTAAAGGCGACCTGAAAAACATGAATCCAGACGGTAAAGGCCGCGTACGTCTCGACTACGTGATCCCAAGCCGTGGTCTGATTGGCttg                   <  1:9962/250‑2 (MQ=255)                             tGATCGACAACATGCGTCGTGAAGGATTCGTACTGGCGGTATCCCGTCCGCAAGTTATCTTCCGTGAAATCGACGGTCGTAAACAAGAGTCGTATGAAAACGTGACTCTGGACGTTGAAGAAAAGCATCAGGGGTCTGTAATGCAGGCGCTGGGCGAACGTAAAGGCGACCTGAAAAACATGAATCCAGACg                                                                           >  1:59732/1‑192 (MQ=255)                              gATCGAAAACATGCGTCGTGAAGGTTTCGAACTGGCGGTATCCCGTCCGAAAGTTATCTTCCGTGAAATCGACGGTCGTAAACAAGAGTCGTATGAAAACGTGACTCTGGACGTTGAAGAACAGCATCAGGGTTCTGTAATGCAGGCGCTGGGCGAACGTAAAGGCGACCTGAAAAACATGAATCCAGACGGTAAAGGCCGCGTACGTCTCGACTACGTGATCCCAAGCCGTGGTCTGATTGGCTTCCGt                <  1:190042/250‑1 (MQ=255)                              gATCGAAAACATGCGGCGTGAAGGTTTCGAACTTGCGGTATCCCGTCCGAAAGTTATCTTCCGTGAAATCGACGGTCGTAAACAAGCTTCTTATGAAAACGTGTCTCTGGACGTTgaag                                                                                                                                                   <  1:177001/119‑1 (MQ=255)                                  gAAAACATGCGTCGTGGAGGTTTAGAACTGGCGGTATCCCGACCGAAAGTTGTCTTCCGTGAAATGGACGGGCGTAAATAAGAGTCGTATGAATAAAAGACGCTGGAGGTTGAAGGGGAACATGAGGGTTCTTTAAAGCAGGCGCCGGGCGAACGTAAAGGCGACCTGAAAAAAATGCAGCCAGCCGGTAAAGGCCGAGCACGGCTCGACTACGCGATCCCAAACCGTGGTCGGATTGGCTTCCGTTCTgg           >  1:70136/1‑250 (MQ=255)                                           cgtcgtGAAGGTTTCGAACTGGCGGTATCCCGTCCGAAAGTTATCTTCCGTGAAATCGACGGTCGTAAACAAGAGTCGTATGAAAACGTGACTCTGGACGTTGAAGAACAGCATCAGGGTTCTGTAATGCAGGCGCTGGGCGAACGTAAAGGCGACCTGAAAAACATGAATCCAGACGGTAAAGGCCGCGTACGTCTCGACTACGTGATCCCAAGCCGTGGTCTGATTGGCTTCCGTTCTGAGTTcatgac  <  1:103206/251‑1 (MQ=255)                                                                                                                      |                                                                                                                                                                                GGTCGTGGCGAACTGCACCTGTCTGTTCTGATCGAAAACATGCGTCGTGAAGGTTTCGAACTGGCGGTATCCCGTCCGAAAGTTATCTTCCGTGAAATCGACGGTCGTAAACAAGAGCCGTATGAAAACGTGACTCTGGACGTTGAAGAACAGCATCAGGGTTCTGTAATGCAGGCGCTGGGCGAACGTAAAGGCGACCTGAAAAACATGAATCCAGACGGTAAAGGCCGCGTACGTCTCGACTACGTGATCCCAAGCCGTGGTCTGATTGGCTTCCGTTCTGAGTTCATGAC  >  NC_000913/4059484‑4059776

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑