Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,055,452	C→T	L296L (TTG→TTA)	glnL ←	protein histidine kinase NtrB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,055,452	0	C	T	100.0%	24.1 / NA	10	L296L (TTG→TTA)	glnL	protein histidine kinase NtrB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (4/6);  total (4/6)

CGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGCCTGAATGCTGATCAATCAAATTACGAGCGATGGATAAGCCAAGCCCGGTGCCACCTTCGCGGCCGCTGACCATCGGGTAAAACAGCGTATCCTGCAAATGAGGCGGAATGCCCGGCCCGTTATCTTCCACATCAATCCGCGCCGCCAGCCGGTAGCGCTCGCCGTGTAAGGTCAGTTGAAACGCGGTGCGGGTACGCAGAATGATTTCACCGCCTTCCGGCCCCAGCGCCTGTAGCGCATTGCGCACAATATTCAGCAAGACCTGTTCAATTTGATCCGGGTCGTGCGCCAGTTCCGGTAGGCTGGGATCGTAATCACGAAT  >  NC_000913/4055245‑4055679                                                                                                                                                                                                                |                                                                                                                                                                                                                                    cGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCGTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGCCTGAATGCAGATCAATCAAATTACGAGCGATGGATAAGCCAAGCCCGGTGCCACCTTCGCGGCCGCTGACCATCGGGTAAAACAGCGTATCCTGTAAATGAGGCGGAATGCCCGGCCCGTTATCTTCCACATCAATc                                                                                                                                                                                           <  1:29121/250‑1 (MQ=255)                              tGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGCCTGAATGCTGATCAATCAAATGACGAGCGAGGGATAAGCCAAGCCCGGTGCCGCCTTCGCGGCCGCTGACCATCGGGTAAAACAGCGTATCCTGTAAATGAGGCGGAATGCCCGGCCCGTTATCTTCCACATCAATCCTCGCCGCCAGCCGGTAGCGCTCGCCgtg                                                                                                                                                              <  1:70857/250‑1 (MQ=255)                                                                                    gCCCTGGCCAACTGGTAAATTCAATTTTGCCTGAATGCTGATCAATCAAATTACGAGCGATGGATAAACCAAGCCCGGTGCCACCTTCGCGGCCGCTGACCATCGGGTAAAACAGCGTATCCTGTAAATg                                                                                                                                                                                                                                >  1:167584/1‑130 (MQ=255)                                                                                          nccAACTGGTAAATTCAATTTTGCCTGAATGCTGATCAATCAAATTACGAGCGATGGATAAGCCAAGCCCGGTGCCACCTTCGCGGCCGCTGACCATCGGGTAAAACAGCGTATCCTGTAAATGAGGCGGAATGCCCGGCCCGTTATCTTCCACATCAATCCGCGCCGCCAGCCGGTAGCGCTCGCCGTGTAAGGTCAGTTGAAACGCGGTGCGGGTACGCAg                                                                                                                             >  1:128976/2‑223 (MQ=255)                                                                                              aCTGGTAAATTCAATTTTGCCTGAATGCTGATCAATCACATAACGAGCGCTGGATA‑GCCAAGCCCGGTGCCACCTTCGCGGCCGCTGACCATCGGGTAAAACAGCGTATCCTGTAAATGAGGCGGAATGCCCGGCCCGTTATCTTCCACATCAATCCGCGCCGCCAGCCGGTAGCGCTCGCCGTGTAAGGTCAGTTGAAACGCGGTGCGGGTACGCAGAATGCTTTCACCGCCTTCCGGCCCCAGCGCCTg                                                                                            >  1:88347/1‑251 (MQ=255)                                                                                                         cAATTTTGCCTGAATGCTGATCAATCAAATTACGAGCGATGGATAAGCCAAGCCCGGTGCCACCTTCGCGGCCGCTGACCATCGGGTAAAACAGCGTATCCTGTAAATGAGGCGGAATGCCCGGCCCGTTATCTTCCACATCAATCCGCGCCGCCAGCCGGTAGCGCTCGCCGTGTAAGGTCAGTTGAAACGCGGTGCGGGTACGCAGAATGATTTCACCGCCTTCCGGCCCCAGCGCCTGTAGCGCAtt                                                                                   <  1:121465/250‑1 (MQ=255)                                                                                                                                            gCGATGGATAAGCCAAGCCCGGTGCCACCTTCGCGGCCGCTGACCATCGGGTAAAACAGCGTATCCTGTAAATGAGGCGGAATGCCCGGCCCGTTATCTTCCACATCAATCCGCGCCGCCAGCCGGTAGCGCTCGCCGTGTAAGGTCAGTTGAAACGCGGAGCGGGTACGCAGAATGATTTCACCGCCTTCCGGCCCCAGCGCCTGTAGCGCATTGCGCACAATATTCAGCAAGACCTGTTCAATTTGATc                                               >  1:3098/1‑251 (MQ=255)                                                                                                                                                       gccaagccCGGTGCCACCTTCGCGGCCGCTGACCATCGGGTAAAACAGCGTATCCTGTAAATGAGGCGGAATGCCCGGCCCGTTATCTTCCACATCAATCCGCGCCGCCAGCCGGTAGCGCTCGCCGTGTAAGGTCAGTTGAAACGCGGTGCGGGTACGCAGAATGATTTCACCGCCTTCCGGCCCCAGCGCCTGTAGCGCATTGCGCACAATATTCAGCAAGACCTGTTCAATTTGATCCGGGTCGTgcg                                    <  1:48906/251‑1 (MQ=255)                                                                                                                                                                   nnnnnnttNNNNNCCGNTGACCATCGGGTAAAACAGCGTATCCTGTAAATGAGGCGGAATGCCCGGCCCGTTATCTTCCACATCAATCCGCGCCGCCAGCCGGTAGCGCTCGCCGTGTAAGGTCAGTTGAAACGCGGTGCGGGTACGCAg                                                                                                                             <  2:128976/144‑1 (MQ=255)                                                                                                                                                                                         aTCGGGTAAAACAGCGTATCCTGTAAATGAGGCGGAATGCCCGGCCCGTTATCTTCCACATCAATCCGCGCCGCCAGCCGGTAGCGCTCGCCGTGTAAGGTCAGTTGTAACGCGGTGCGGGTACGCAGAATGATTTCTCCGCCTTCCGGCCCCAGCGCCTGTAGCGCATTGCGCACAATATTCAGCAAGACCTGTTCAATTTGATCCGGTTCGTGCGCCAGTTCCGGTAGGCTGGGATCGTAATCACGAAt  <  1:124836/251‑1 (MQ=255)                                                                                                                                                                                                                |                                                                                                                                                                                                                                    CGTCATCGACTACCCAGACTATCCCTCGTTGCATAAACGTCACCTTTATTTCCTGATAGGCAGGTAAACCGAGAACTCGGTATGCCCTGGCCAACTGGTAAATTCAATTTTGCCTGAATGCTGATCAATCAAATTACGAGCGATGGATAAGCCAAGCCCGGTGCCACCTTCGCGGCCGCTGACCATCGGGTAAAACAGCGTATCCTGCAAATGAGGCGGAATGCCCGGCCCGTTATCTTCCACATCAATCCGCGCCGCCAGCCGGTAGCGCTCGCCGTGTAAGGTCAGTTGAAACGCGGTGCGGGTACGCAGAATGATTTCACCGCCTTCCGGCCCCAGCGCCTGTAGCGCATTGCGCACAATATTCAGCAAGACCTGTTCAATTTGATCCGGGTCGTGCGCCAGTTCCGGTAGGCTGGGATCGTAATCACGAAT  >  NC_000913/4055245‑4055679

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑