Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,049,777 C→T intergenic (+25/‑122) polA → / → spf DNA polymerase I/small regulatory RNA Spot 42

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,049,7770CT100.0% 29.4 / NA 11intergenic (+25/‑122)polA/spfDNA polymerase I/small regulatory RNA Spot 42
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (5/6);  total (5/6)

TGAAGTTCATAAAGATGATGTTGATGCCGTCGCGAAGCAGATTCATCAACTGATGGAAAACTGTACCCGTCTGGATGTGCCGTTGCTGGTGGAAGTGGGGAGTGGCGAAAACTGGGATCAGGCGCACTAAGATTCGCCTGAACATGCCTTTTTTCGTAAGTAAGCAACATAAGCTGTCACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGG  >  NC_000913/4049623‑4049998
                                                                                                                                                          |                                                                                                                                                                                                                             
tGAAGNNCANNNAGNNGATGTTGATGCCGTCGCGAAGCANATTCATCAACTGATGGAAAACTGTACCCGNCNGNATGTNNCGTNGCTGGTGGAANTGGGGAGTGGCGAAAACTGGGATCAGGCGCACTAAGATTCGCCTGAACNTNCCTTTTTTTGTAAGTAAGCAACATAAGCTGTNACGTTTTGTGATGGCTATTAGAAANTCCTNNGcnan                                                                                                                                                                    >  2:164769/1‑213 (MQ=255)
                       aTGCCGTCGCGAAGCAGATTCATCAACTGATGGAAAACTGTACCCGTCTGGATGTGCCGTTGCTGGTGGAAGTGGGGAGTGGCGAAAACTGGGATCAGGCGCACTAAGATTCGCCTGAACATGCCTTTTTTTGTAAGTAAGCAACATAAGCTGTCACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTctgaac                                                                                                                                 <  1:90935/226‑1 (MQ=255)
                                           catcaACTGATGGAAAACTGTACCCGTCTGGATGTGCCTTTGCTGGTGGAAGTGGTGAGTGGCGAAAACTGGGATCAGGCGCACTAAGATTCGCCTGAACATGCCTTTTTTTGTAAGTAAGCAACATAAGCTGTCACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTTGTCGCTTAGGGTACAGAGGTAAg                                                                                    <  1:177807/251‑1 (MQ=255)
                                                                                                  ggAGTGGCGAAAACTGGGATCAGGCGCACTAAGATTCGCCTGAACATGCCTTTTTTTGTAAGTAAGCAACATAAGCTGTCACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACNAAANNNNNttnn                                                                                                                                      >  2:133537/1‑144 (MQ=255)
                                                                                                  ggAGTGGCGAAAACTGGGATCAGGCGCACTAAGATTCGCCTGAACATGCCTTTTTTTGTAAGTAAGCAACATAAGCTGTCACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGc                                                                                                                                          <  1:185924/142‑1 (MQ=255)
                                                                                                        gCGAAAACTGGGATCAGGCGCACTAAGATTCGCCTGAACATGCCTTTTTTTGTAAGTAAGCAACATAAGCTGTCACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAgccgc                       >  1:46161/1‑251 (MQ=255)
                                                                                                                            cACTAAGATTCGCCTGAACATGCTTTTTTTTGTAAGTAAGCAACATAAGCTGTCACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAA‑TTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACTTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTGGCCGCCCCAGTCAGTAATGACTgggg  <  1:183446/251‑1 (MQ=255)
                                                                                                                            cACTAAGATTCGCCTGAACATGCCTTTTTTTGTAAGTAAGCAACATAAGCTGTCACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTggg   >  1:47573/1‑251 (MQ=255)
                                                                                                                                      cGCCTGAANANNNCNNTTTTTGTAAGTAAGCAACATAAGCTGTCACGTTTTGTGATGGCTNTTAGAAATTCCTATGNAANAACTNAAANAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAg                                                                                                  >  2:149161/1‑146 (MQ=255)
                                                                                                                                      cGCCTGAACATGCCTGTTTTTGTAAGTAAGCAACATAAGTTTTCACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAg                                                                                                  <  1:149161/146‑1 (MQ=255)
                                                                                                                                       gCCTGAACATGCCTTTTTTTGTAAGTAAGCAACATAAGCTGTCACGTTTTGTGATGGCTATTAGAAATGCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACGTCACGTAATCGGATTTg                                         <  1:68606/202‑1 (MQ=255)
                                                                                                                                                          |                                                                                                                                                                                                                             
TGAAGTTCATAAAGATGATGTTGATGCCGTCGCGAAGCAGATTCATCAACTGATGGAAAACTGTACCCGTCTGGATGTGCCGTTGCTGGTGGAAGTGGGGAGTGGCGAAAACTGGGATCAGGCGCACTAAGATTCGCCTGAACATGCCTTTTTTCGTAAGTAAGCAACATAAGCTGTCACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGG  >  NC_000913/4049623‑4049998

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑