Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,047,278	C→T	P105S (CCG→TCG)	polA →	DNA polymerase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,047,278	0	C	T	100.0%	27.5 / NA	11	P105S (CCG→TCG)	polA	DNA polymerase I
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (9/2);  total (9/2)

GTTTCCCCCGCTGACTAACAGCGCAGGCGAGCCGACCGGTGCGATGTATGGTGTCCTCAACATGCTGCGCAGTCTGATCATGCAATATAAACCGACGCATGCAGCGGTGGTCTTTGACGCCAAGGGAAAAACCTTTCGTGATGAACTGTTTGAACATTACAAATCACATCGCCCGCCAATGCCGGACGATCTGCGTGCACAAATCGAACCCTTGCACGCGATGGTTAAAGCGATGGGACTGCCGCTGCTGGCGGTTTCTGGCGTAGAAGCGGACGACGTTATCGGTACTCTGGCGCGCGAAGCCGAAAAAGCCGGGCGTCCGGTGCTGATCAGCACTGGCGATAAAGATATGGCGCAGCTGGTGACGCCAAATATTACGCTTATCAATACCATGACGAATACCATCCTCGGACCGGAAGAGGTGGTGAATAAGTACGGCGTGCCGCCAGAACTGATCATCGATTTCCTGGCGCTGATGGGTG  >  NC_000913/4047037‑4047518                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                 gTTTCCCCCGCTGACTAACAGCGCAGGCGAGCCGACCGGTGCGATGTATGGTGTCCTCAACATGCTGCGCAGTCTGATCATGCAATATAAACCGACGCATGCAGCGGTGGTCTTTGACGCCAAGGGAAAAACCTTTCGTGATGAACTGTTTGAACATTACAAATCACATCGCCCGCCAATGCCGGACGATCTGCGTGCACAAATCGAACCCTTGCACGCGATGGTTAAAGCGATGGGACTGTCGCTGCTgg                                                                                                                                                                                                                                         >  1:16344/1‑251 (MQ=255)  tttCCCCCGCTGACTAACAGCGCAGGCGAGCCGACCGGGGCGATGTATGGTGTCCTCAACATGCTGCGCAGTCTGATCATGCAATATAAACCGACGCATGCAGCGGTGGTCTTTGACGCCAAGGGAAAAACCTTTCGTGATGAACTGTTTGAACATTACAAATAACATCGCCCGCCACTGCCGGACGATCTCAGTGCACCACACGACACCTTGCACGCGATGGTTAAAGCGATGGGACTGTCGCTGCTGGc                                                                                                                                                                                                                                        >  1:81841/1‑251 (MQ=255)                                  naCCGGTGCGATGTATGGTGTCCTCAACATGCTGCGCAGTCTGATCATGCAATATAAACCGACGCATGCAGCGGTGGTCTTTGACGCCAAGGGAAAAACCTTTCGTGATGAACTGTTTGAACATTACAAATCACATCGCCCGCCAATGCCGGACGATCTGCGTGCACAAATCGAACCCTTGCACGCGATGGTTAAAGCGATGGGACTGTCGCTGCTGGCGGTTTCTTGCGtcagatcggaaaggcacacgt                                                                                                                                                                                                        >  1:55719/2‑231 (MQ=255)                                                aTGGTGTCCTCAACATGCTGCGCAGTCTGCTCATGCAATATAAACCGACGCATGCAGCGGTGGTCTTTGACGCCAAGGGAACAACCTTTCGTGATGAACTGTTTGAACATTACAAATCACATCGCCCGCCAATGCCGGACGATCTGCGTGCACAAATCGAACCCTTGCACGCGATGGTTAAAGCGATGGGACTGTCGCTGCTGGCGGTTTCTGGCGTAGAAACGGACGACGGTATCGGTACTTTGgcgcgc                                                                                                                                                                                          >  1:105202/1‑251 (MQ=255)                                                                           tGATCATGCAATATAAACCGACGCATGCAGAGGTGGTCTTTGACGCCAAGGGAAAAACCTTTCGTGACGCACTGTTTGAACAT‑ACACCTCACATCGCCCGCAAATGCCGGACGATCTGCGCGCACAAATCGAACCCTTGCACGCGATGGTTAAAGCGATGGGACTGTCGCTGCTGGCGGTTTCTGGCGTCGAAGCGGAGGACGTTTTCGGTCCAATGGCGCGCGAAACCGAAAAAGCCAGGCgacaggggc                                                                                                                                                              >  1:91021/1‑243 (MQ=255)                                                                                  gCAATATAAACCGACGCATGCAGCGGTGGTCTTTGACGATAAGGGAAAAACCTTTCGTGATGAACTGTTTGAACATTACAAATCACATCGCCCGCCAATGCCGGACGATCTGCGTGCACAAATCGAACCCTTGCACGCGATGGTTAAAGCGATGGGACTGTCGCTGCTGGCGGTTTCTGGCGTAGAAGCGGACGACGTTATCGGTACTCTGGCGCGCGAAGCCGAAAAAGcc                                                                                                                                                                           >  1:75400/1‑232 (MQ=255)                                                                                                                     aCGCCAAGGGAAAAACCTTTCGTGATGACCTGTTTGAACATTACAAACCACAACGCCCGCCAATGCCGGACGATCTGCGTGCACAAATTGAACCCTTGCACGCGATGGTTAAAGCGATGTGACTGTCGCTGCTGTCGGTTTCTGGCGTAGAAGCGTACGACGTTATCGGTACTCGGGCGCGCGAAGCCGAAAAAGCCTGGCGTCCGGTGCTGATCAGCACTTGTGATAAAGATATTGCGCATCTGTTGACg                                                                                                                     <  1:66904/251‑1 (MQ=255)                                                                                                                                                                         tCGCCCGCCAATGCCGGACGATCTGCGTGCACAAATCGAACCCTTGCACGCGATGGTTAAAGCGATGGGCCTGTCGCTGCTGGCGGTTTCTGGCGTAGAAACGGACGGCTTTATCGGAACTCTTGCGGGCGGAGCCGAAACAGCCGGGCGTCCGGGGCTGCGCAGCACTGGCGATAAAGATATGGCGCAGCTGGGGGCGTCCAATATTACGCTTCTCAATACCATGACGAATACCATCCTCGGACCGGa                                                                   >  1:115366/1‑249 (MQ=255)                                                                                                                                                                            cccgccAATGCCGGACGATCTGCGTGCACAAATCGAACCCTTGCACGCGATGGTTACAGAGATGGGACTGTCGCTGCTGGCGGTTTCTGGCGTAGAAGCGGACGACGCTATCGGTAATCTAGCGCGCGAAGCCGAAAAAGCCGGGCGTCCGGTGCTGGTCCGCACTGGCGCTAAAGATATGGCGCAGCTGGTGACGCCAAATATTACGCTTATCAATACCATGAAGAATACCATCCTCGGACCGGAAGAgg                                                              >  1:143164/1‑251 (MQ=255)                                                                                                                                                                                                  cGTGCACAAATCGAACCCTTGCACGCGATGGTTAAAGCGATGGGACTGTCGCTGCTGGCGGTTTCTGGCGTAGAAGCGGACGACGTTATCGGTACTCTGGCGCGCGAAGCCGAAAAAGCCGGGCGTCCGGTGCTGATCAGCACTGGCGATAAAGATATGGCGCAGCTGGAGACGCCAAATATTACGCTTATCAATACCATGACGAATACCATCCTCGGACCGGAAGAGGGGGTGAATAAGTACGGCGTgcc                                        >  1:183023/1‑251 (MQ=255)                                                                                                                                                                                                                                        gATGGGACTGTCGCTGCTGGCGGTTTCTGGCGTAGAAGCGGACGACGTTATCGGTACTCTGGCGCGCGAAGCCGAAAAAGCCGGGCGTCCGGTGCTGATCAGCACTGGCGATAAAGATATGGCGCAGCTGGTGACGCCAAATATTACGCTTATCAATACCATGACGAATACCATCCTCGGACCGGAAGAGGTGGTGAATAAGTACGGCGTGCCGCCAGAACTGATCATCGATTTCCTGGCGCTGATGGGTg  <  1:12393/251‑1 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                 GTTTCCCCCGCTGACTAACAGCGCAGGCGAGCCGACCGGTGCGATGTATGGTGTCCTCAACATGCTGCGCAGTCTGATCATGCAATATAAACCGACGCATGCAGCGGTGGTCTTTGACGCCAAGGGAAAAACCTTTCGTGATGAACTGTTTGAACATTACAAATCACATCGCCCGCCAATGCCGGACGATCTGCGTGCACAAATCGAACCCTTGCACGCGATGGTTAAAGCGATGGGACTGCCGCTGCTGGCGGTTTCTGGCGTAGAAGCGGACGACGTTATCGGTACTCTGGCGCGCGAAGCCGAAAAAGCCGGGCGTCCGGTGCTGATCAGCACTGGCGATAAAGATATGGCGCAGCTGGTGACGCCAAATATTACGCTTATCAATACCATGACGAATACCATCCTCGGACCGGAAGAGGTGGTGAATAAGTACGGCGTGCCGCCAGAACTGATCATCGATTTCCTGGCGCTGATGGGTG  >  NC_000913/4047037‑4047518

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑