Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	778,709	C→T	P324S (CCA→TCA)	tolB →	Tol‑Pal system periplasmic protein TolB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	778,709	0	C	T	100.0%	12.5 / NA	9	P324S (CCA→TCA)	tolB	Tol‑Pal system periplasmic protein TolB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (4/5);  total (4/5)

CGCGTCACAACGGTGCACCTGCATTCTCGCCAGACGGCAGCAAACTGGCATTCGCCTTGTCGAAAACCGGTAGTCTGAACCTGTACGTAATGGATTTGGCTTCTGGTCAGATCCGCCAGGTGACTGATGGTCGCAGTAACAATACCGAACCGACCTGGTTCCCGGATAGCCAGAACCTGGCATTTACTTCTGACCAGGCCGGTCGTCCGCAGGTTTATAAAGTGAATATCAACGGCGGTGCGCCACAACGTATTACCTGGGAAGGTTCGCAGAACCAGGATGCGGATGTCAGCAGCGACGGTAAATTTATGGTAATGGTCAGCTCCAATGGTGGGCAGCAGCACATTGCCAAACAAGATCTGGCAACGGGAGGCGTACAAGTTCTGTCGTCCACGTTCCTGGATGAAACGCCAAGTCTGGCACCTAACGGCACTATGGTAATCTACAGCTCTTCTCAGGGGATGGGATCCG  >  NC_000913/778467‑778937                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                     cgcgTCACAACGGTGCACCTGCATTCTCGCCAGACGGCAGCAAACTGGCATTCGCCTTGTCGAAAACCGGTAGTCTGAACCTGTACGTAATGGATTTGGCTTCTGGTCAGATCCGCCAGGTGACTGCTGGTCGCAGTAACAATACCGAACCGACCTGGTTCCCGGATAGCCAGAACCTGGCATTTACTTCTGACCAGGCCGGTCGTCCGCAGGTTTATAAAGTGAATATCAACGGCGGTGCGTCACAACGt                                                                                                                                                                                                                              >  1:102640/1‑251 (MQ=255)                                                                                            ggATTTGGCTTCTGGTCAGATCCGCCAGGTGACTGCTGAACGCAGTAAAACTCCCAAACCGACCTGGTTCACGGATCGACAGCACCTGACATTTACTTCTGACCAGACCGGTCGTCCGCAGGTTTATAAAGTGAATATCAACGGCGGTGCGTCCCAACGTATTACCTGGGAAGGTTCGCAGAACCAGGATGCGGGTTTCGGCAGCGACGGTAAATTTATGGTGATGGTCCGCTCCACTGGTGGgcagcagc                                                                                                                                   >  1:35478/1‑251 (MQ=255)                                                                                                    cTTCTGGTCAGATCCGCCAGGTGACTGATGGTCGCAGTAACAATACCGAACCGAACTGGTTCCAGGATAGCCAGAAACTGGCATTTACTTCTGACCAGGCCGGTCGTCCGCAGGTTTATAAAGTGAATATCAACGGCGGTGCGTCACAACGTATTACCTGGGAAGGTTCGCAGAACCAGGATGCGGATGTCAGCAGCGACGGTAAATTTATGGTAATGGGCAGCTCCAATGGTGGGCAGCAGCACATTGcc                                                                                                                           >  1:76477/1‑251 (MQ=255)                                                                                                          gTCAGATCCGCCAGGTGACTGATGGTCGCAGTAACAATACCGAACCGACCTGGTTCCCGGATAGCCAGAACCTGGCATTTACTTCTGTCCATGCCGGTCGTCCGCAGGTTTATAAAGTGAATATCAACGGCGGTGCGTCACAACGTATTACCTGGGAAGGTTCGCAGAACCAGGATGCGGATGTcagcagag                                                                                                                                                                                <  1:210873/192‑3 (MQ=255)                                                                                                                        gTGACTGATGGTCGCAATAACAATACCGAACCTACCTGGTCCCCGGATAGCCAGAACCTGACATTTACTTCTGACCAGGCCGGTCGTCCGCAGGTTTATAAAGGGAATAGCAACGGCGGTGCGTCACAACTTATTACCTGGGAAGGTACGCAGAACCAGGATGCGGATGTCAGCAGCGACGGTAAATTTATGATAATGGTCAGCTCCAATGGTGGGCAGCAGCACATTGCCAAACAAGATCTGGCAACggg                                                                                                       <  1:150691/251‑1 (MQ=255)                                                                                                                                                        gaCCTGGTTCCCGGATAGCCAGAACCTGGCATTTCCTTCTGAGCGGGCCGGTGGTCCGCAGGTTTAGAAAGTGAATATCAACGGCGGTGCGTCACAAGTTATTACCGGGGAATGTTCGCAGAACCAGGAGGCGGATGGCAGCAGCGACTTTAAATTTATGGTAATGGTCAGCTCCAATGGTGGgcagcag                                                                                                                                    <  1:221676/190‑1 (MQ=255)                                                                                                                                                                                 cTGGCATTTACTTCTGACCAGGCCGGTCGTCCGCAGGTTTATAAAGTGAATATCCACGGCGGTGCGTCACAACGTATTACCTGGGAAGGTTCGCAGAACCAGGATGCGGCTGTCAGCAGCGACGGTAAATTTATGGTAAt                                                                                                                                                             >  1:207172/1‑140 (MQ=255)                                                                                                                                                                                                          gtcgtcCGCAGGTTTATAAAGTGAATATCAACGGCGGTGCGTCACAACGTATTACCTGGGAAGGTTCGCAGAACCAGGATGCGGATGTCAGCAGCGACGGTAGATTTATGGTAATGGTCAGCTCCAATGGTGGGCAGCAGCACATTGCCAAACAAGATCTGGCAACGGGATGCGTACAAGTTCTGTCGTCCACGTTCCTGGATGAAACGCCAAGTCTGGCACCTAACGGCACTATTGTAATCTACAGctct                     <  1:215472/251‑1 (MQ=255)                                                                                                                                                                                                                             aGTGAATATCAACGGCGGTGCGTCACAACGTATTACCTGGGAAGGTTCGCAGAACCAGGATGCGGATGTCAGCAGCGACGGTAAATTTATGGTAATGGTCAGCTCCAATGGTGGGCAGCAGCACATTGCCAAACAAGATCTGGCAACGGGAGGCGTACAAGTTCTGTCGTCCACGTTCCTGGATGAAACGCCAAGTCTGGCACCTAACGGCACTATGGTAATCTACAGCTCTTCTCAGGGGATGGGATCCg  <  1:2119/251‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                     CGCGTCACAACGGTGCACCTGCATTCTCGCCAGACGGCAGCAAACTGGCATTCGCCTTGTCGAAAACCGGTAGTCTGAACCTGTACGTAATGGATTTGGCTTCTGGTCAGATCCGCCAGGTGACTGATGGTCGCAGTAACAATACCGAACCGACCTGGTTCCCGGATAGCCAGAACCTGGCATTTACTTCTGACCAGGCCGGTCGTCCGCAGGTTTATAAAGTGAATATCAACGGCGGTGCGCCACAACGTATTACCTGGGAAGGTTCGCAGAACCAGGATGCGGATGTCAGCAGCGACGGTAAATTTATGGTAATGGTCAGCTCCAATGGTGGGCAGCAGCACATTGCCAAACAAGATCTGGCAACGGGAGGCGTACAAGTTCTGTCGTCCACGTTCCTGGATGAAACGCCAAGTCTGGCACCTAACGGCACTATGGTAATCTACAGCTCTTCTCAGGGGATGGGATCCG  >  NC_000913/778467‑778937

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑