Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,019,367	C→T	F171F (TTC→TTT)	ubiE →	bifunctional 2‑octaprenyl‑6‑methoxy‑1,4‑benzoquinol methylase and demethylmenaquinone methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,019,367	0	C	T	100.0%	35.3 / NA	13	F171F (TTC→TTT)	ubiE	bifunctional 2‑octaprenyl‑6‑methoxy‑1,4‑benzoquinol methylase and demethylmenaquinone methyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (9/4);  total (9/4)

GCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGACAAAGATAAAGCACTGCGTTCAATGTATCGCGTGCTGAAACCCGGCGGCCGCCTGCTGGTGCTTGAGTTCTCGAAGCCAATTATCGAGCCGCTGAGCAAAGCCTATGATGCATACTCCTTCCATGTGCTGCCGCGTATTGGCTCACTGGTCGCGAACGACGCCGACAGCTACCGTTATCTGGCAGAATCCATCCGTATGCATCCCGATCAGGATACCCTGAAAGCCATGATGCAGGATGCCGGATTCGAAAGTGTCGACTACTACAATCTGACGGCAGGGGTTGTGGCGCTGCATCGTG  >  NC_000913/4019120‑4019596                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                      gCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGACAAAGATAAAGCACTGCGTTCAATGTATCGCGTGCTGAAACCCGGCGGCCGCCTACTGGTGCTTGAGTTTTCg                                                                                                                                                                                                                                    <  1:33123/251‑1 (MQ=255)                                                                          gATTGGTAACGTTGAGTCTGTTACGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGACAAAGATAAAGCACTGCGTTCAATGTATCGCGTGCTGAAACCCGGCGGCCGCCTGCTGGTGCTTGAGTTTTCGAAGCCAATTATCGAGCCGCTGAGc                                                                                                                                                                                                            >  1:58435/1‑202 (MQ=255)                                                                                                                                 aaCACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGACACCGACAAAGATAAAGCACTGCGTTCAATGTATCGCGTGCTGAAACCCGGCGGCCTCCTGCTGGTGCTTGAGTTTTTGAAGCCAATTATCGAGCCGCTTAGCAAAGCCTATGATGCATACTCCTTCCATGTGCTGCGGCGGATTGGCTCACGGTTCGCGAACGACGCCGACAGCTACCGTTATCTGGCAGAATCCATCCGTATGc                                                                                                     <  1:117131/250‑1 (MQ=255)                                                                                                                                                                     cGTAACGTCACCGACAAAGATAAAGCACTGCGTTCAATGTATCGCGTGCTGAAACCCGGCGGCCGCCTGCTGGTGCTTGAGTTTTCGAAGCCAATTATCGAGCCGCTGAGCAAAGCCTATGATGCATACTCCTTCCATGTGCTGCCGCGTATTGGCTCACTGGTCGCGAACGACGCCGACAGCTACCCTTATCTGGCAGAATCCATCCGTATGCATCCCGATCAGGATACCCTGAAAGCCATGATGCAgg                                                                 >  1:183776/1‑250 (MQ=255)                                                                                                                                                                          ngTCACCGACAAAGATAAAGCACTGCGTTCAATGTATAGCGTGCTGAAACCCGGCGGCCGCCTGCTGGTGCTTGAGTTTTCGAAGCCAATTATCGAGCCGCTGAGCAAAGCCTATGATGCATACTCCTTCCATGTGCTGCCGCGTATTGGCTCACTGGTCGCGAACGACGCCGACAGCTACCGTTATCTGGCAGAATCCATCCGTATGCATCCCGATCAGGATACCCTGAAAGCCATGATGCAGGATGCCg                                                           >  1:44174/2‑251 (MQ=255)                                                                                                                                                                                   cAAAGATAAAGCACTGCGTTCAATGTATCGCGTGCTGAAACCCGGCGGCAGCCTGCTGGAGCTTGAGTTTTAGAAGCCAATTATCGAg                                                                                                                                                                                                                     >  1:198045/1‑88 (MQ=255)                                                                                                                                                                                                       naaTGTATCGCGTGCTGAAACCCGGCGGCCGAATGCTGGTGCTTGCGTTTTCGAAGCAACATATCGAGCCGATCAGCAAAGCCTACGATGCCAACTCCTTCCATGTGCTCCCGAGTCTTGGCTCACTGGTCGCGAACGACGCCGACAGCTACCGTTATCTGGCAGAATCCATCAGTAAGCATCCCGCTCAGGATACCCTGAAAGCCATACTGCAGGATGCCGTATTCGAAAGTGACGACTACTACAATCTg                              >  1:24657/2‑251 (MQ=255)                                                                                                                                                                                                       cAATGTATCGCGTGCTGAAACCCGGCGGCCGCCTGCTGGTGCTTGAGTTTTCGAAGCCAATTATCGAGCCGCTGAGCAAAGCCTATGATGCATACTCCTTCCATGTGCTGCCGCGTATTGGCTCACTGGTCGCGAACGACGCCGACAGCTACCGTTATCTGGCAGAATCCATCCGTATGCATCCCGATCAGGATACCCTGAAAGCCATGATGCAGGATGCCGGATTCGAAAGTGTCGACTACTACAATCTg                              >  1:66405/1‑251 (MQ=255)                                                                                                                                                                                                                                   ncgccTGCTGGTGCTTGAGTTTTCGAAGCCAATTATCGAGCCGCTGAGCAAAGCCTATGATGCATACTCCTTCCATGTGCTGCCGCGTATTGGCTCACTGGTCGCGAacgacg                                                                                                                                            >  1:27132/2‑113 (MQ=255)                                                                                                                                                                                                                                   ccgccTGCTGGTGCTTGAGTTTTCGAAGCCAATTATCGAGCCGCTGAGCATAGCCTATGATGCATACTCCTTCCATGTGCTGCCGCGTATTGGCTCACTGGTCGCGAACGACGCCGACAGCTACCGTTATCTGGCAGAATCCATCCGTATGCATCCCGATCAGGATACCCTGAAAGCCATGATGCAGGATGCCGGATTCGAAAGTGTCGACTACTACAATCTGACGGCAGGGGTTGTGGCGCTGCATCGtn  <  1:55205/251‑2 (MQ=255)                                                                                                                                                                                                                                     gccTGCTGGTGCTTGAGTTTTCGAAGCCAATTATCGAGCCGCTGAGCAAAGCCTATGATGCATACTCCTTCCAtn                                                                                                                                                                                <  1:134547/75‑2 (MQ=255)                                                                                                                                                                                                                                     gccTGCTGGTGCTTGAGTTTTCGAAGCCAATTATCGAGCCGCTGAGCAAAGCCTATGATGCATACTCCTTCCAtg                                                                                                                                                                                >  2:134547/1‑75 (MQ=255)                                                                                                                                                                                                                                                 ttGAGTTTTCGAAGCCAATTATCGAGCCGCTGAGCAAAGCCTATGATGCATACTCCTTCCCTGTGCTGCCGCGTATTGGCTCACTGGTCGCGAACGAAGCCGACAGCTAc                                                                                                                                 >  1:106039/1‑110 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                      GCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGACAAAGATAAAGCACTGCGTTCAATGTATCGCGTGCTGAAACCCGGCGGCCGCCTGCTGGTGCTTGAGTTCTCGAAGCCAATTATCGAGCCGCTGAGCAAAGCCTATGATGCATACTCCTTCCATGTGCTGCCGCGTATTGGCTCACTGGTCGCGAACGACGCCGACAGCTACCGTTATCTGGCAGAATCCATCCGTATGCATCCCGATCAGGATACCCTGAAAGCCATGATGCAGGATGCCGGATTCGAAAGTGTCGACTACTACAATCTGACGGCAGGGGTTGTGGCGCTGCATCGTG  >  NC_000913/4019120‑4019596

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑