Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,007,812	C→T	P19L (CCC→CTC)	rhtC →	L‑threonine exporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,007,812	0	C	T	100.0%	41.2 / NA	16	P19L (CCC→CTC)	rhtC	L‑threonine exporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (8/8);  total (8/8)

CAGCGAAATGCTCAGCGTTAACGGCGTTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAGTCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGTAAACGTTTCCCCCGCGAGTCAAATGTATGTTGATGTTATTTCTCACCGTCGCCATGGTGCACATTGTGGCGCTTATGAGCCCCGGTCCCGATTTCTTTTTTGTCTCTCAGACCGCTGTCAGTCGTTCCCGTAAAGAAGCGATGATGGGCGTGCTGGGCATTACCTGCGGCGTAATGGTTTGGGCTGGGATTGCGCTGCTTGGCCTGCATTTGATTATCGAAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTT  >  NC_000913/4007585‑4008053                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                  cAGCGAAATGCTCAGCGTTAACGGCGTTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTTCGCATGTTGATGGCGATGACGAGGAGTAGTCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGTAAACGTTTCCCGCGCGAGTCAAATGTATGTTGATGTTATTTCTCACCGTCGCCATGGTGCACATTGTGGCGCTTATGAGCCTCGGTCCCGATTTCTTTTTTGtct                                                                                                                                                                                                                            <  1:218855/251‑1 (MQ=255)             cAGCGTTAACGGCGGTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAGTCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGTAAACGTTTCCCCCGCGAGTCAAATGTATGTTGATGTTATTTCTCACCGTCGCCATGGTGCACATTGTGGCGCTTATGAGCCTCGGTCCCGATTTCTTTTTTGTCTCTCAGACCGc                                                                                                                                                                                                                  <  1:2639/249‑1 (MQ=255)                   tAACGGCGTTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAGTCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGTAAACGTTTCCCCCGCGAGTCAAATGTATGTTGATGTTATTTCTCACCGTCGCCATGGTGCACATTGTGGCGCTTATGAGCCTCGGTCCCGATTTCTTTGTTGTCTCTCAGACCGCTGTCAGt                                                                                                                                                                                                           <  1:190712/250‑1 (MQ=255)                    aaCGGCGTTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAGTCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGTAAACGTTTCCCCCGCGAGTCAAATGTATGTTGATGTTATTTCTCACCGTCGCCATGGTGCACATTGTGGCGCTTATGAGCCTCGGTCCCGATTTCTTTTTTGTCTCTCAGACCGCTGTCAGTc                                                                                                                                                                                                          <  1:62131/250‑1 (MQ=255)                                   cgcAAGCTGGAACGCTTTGGCAATCCGTTTATGGCGCTGATTCGTGCGGATGTTGATGGCGATGACGAAGAGGAGTCAGCAGCAGAAAAAAGTGCCAGTATGAATACTCCGTAAACGGTGCCCCCGCGAGTCAAATGTATCTTGATGTTATTGCTCACCGTCGCCATGGTGCACATTGTGGCGCTTAGGAGCCTCGGTCCCGATTTCTTTTTTGTCTCTCAGACCGCTGTCAGTCGTTCCCGTAAAGAAGc                                                                                                                                                                                          <  1:28883/251‑1 (MQ=255)                                               tccgatcgCAAACCGTTTATGGCGCGGATTCGTGCGCATGTTGATGGCGAGGACGAAGAGTAGTCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGTAAACGTTTCCCCCTCGATTCAAATGTATGTTGATGGTATTTCTCACCGTCGCCAGGGTGCACATTGTGGCGCTTATGAGCCTCTGTCCCTATTTCTTTTTTGTCTCTCAGTCCGCTGTCAGTCGTTCCCGTAAAGAAGCGATGATGGGCg                                                                                                                                                                               <  1:154493/243‑1 (MQ=255)                                                                                                             gtCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGTAAACGTTTCCCCCGCGAGTCAAATGTATGTTGATGTTATTTCTCACCGTCGCCATGGTGCACATTGTGGCGCTTATGAGCCTCGGTCCCGATTTCTTTTTTGTCTCTCAGACCGCTGTCAGTCGTTCCCGTAAAGAAGCGATGATGGGCGTGCTGGGCATTACCTgcgg                                                                                                                                                            >  1:67098/1‑207 (MQ=255)                                                                                                                                gCCAGTATGAAGACTCCGTAAACGTTTCCCCCGCGAGTCAAATGTATGTTGATGTTATTTCTCACCGTCGCCATGGTGCACATTGTGGCGCTTATGAGCCTCGGTCCCGATTTCTTTTTTGTCTCTCAGACCGCTGTCAGTCGTTCCCGTAAAGAAGCGATGATGGGCGTGCTGGGCATTACCTGCGGCGTAATGGTTTGGGCTGGGATTGCGCTGCTTGGCCTGCATTTGATTATCGAAAAAATGGCCTg                                                                                             >  1:173905/1‑251 (MQ=255)                                                                                                                                 ncAGTATGAAGACTCCGTAAACGTTTCCCCCGCGAGTCAAATGTATGTTGATGTTATTTCTCACCGTCGCCATGGTGCACATTGTGGCGCTTATGAGCCTCGGTCCCGATTTCTTTTTTGTCTCTCAGACCGCTGTCAg                                                                                                                                                                                                            >  1:20402/2‑139 (MQ=255)                                                                                                                                                       gTTTCCCCCGCGAGTCAAATGTATGTTGATGTTATTTCTCACCGTCGCCATGGTGCACATTGTGGCGCTGATGAGCCTCGGTCCCGATTTCTTTTTTGTCTCTCAGACCGCTGTCAGTCGTTCCCGTAAAGAAGCGATGATGGGCGTGCTGGGCATTACCTGCGGCGTAATGGTTTTGTCTGTGATGGCGCTGCGTGGCCTGCATTTGATTATCGAAAAAATGGCCTGGCTGCATACGCTGATTAtggtgg                                                                      <  1:211811/251‑1 (MQ=255)                                                                                                                                                             cccGCGAGTCAAATGTATGGTGATGTTATTTCTCACCGTCGCCAGGGTGCACATTGTGGCGCGTATGATCCTCGGTCCCGATTTCTTTTTTGTCTCTCAGACCGCTGTCAGTCGTTCCCGTAAAGAAGCgat                                                                                                                                                                                       <  1:124628/132‑1 (MQ=255)                                                                                                                                                                             atgtTGATGTTATTTCTCACCGTCGCCATGGTGCACATTGTGGCGCTTATGAGCCTCGGACCCGCTTTCTTTTTTGTATCTCAGACCGCTGTCAGTCGTTCCCGTCAAGAAGCGATGCTGGGCGTGCTGGGCATTACCTGCGGCGTAATGGTTTGGGCTGGGATTGCGCTGCTTGGCCTGCATTTGATTATCGAAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTctgctg                                                >  1:114605/1‑251 (MQ=255)                                                                                                                                                                                               aCCGTCGCCATGGTGCACATTGTGGCGCTTATGAGCCTCGGTCCCGATTTCTTTTTTGTCTCTCAGACCGCTGTCAGTCGTTCCCGTAAAGAAGCGATGATGGGCGTGCTGGGCATTACCTGCGGCGTAATGGTTTGGGCTGGGATTGCGCTGCTTGGCCTGCATTTGATTATCGAAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGAATCTCTGCTGGATGGGTTACCAGATGCt                              >  1:17369/1‑251 (MQ=255)                                                                                                                                                                                                          ggTGCACATTGTGGCGCTTATGAGCCTCGGTCCCGATTTCTTTTTTGTCTCTCAGACCGCTGTCAGTCGTTCCCGTAAAGACGAGCTGATGGGCGTGCTGGGCATTACCTGCGGCGTAATGGTTTGGGCTGGGATTGCGCTGCTTGGCCTGCATTTGATTATCGAAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCAc                   >  1:187813/1‑251 (MQ=255)                                                                                                                                                                                                                        cgcTTATGAGCCTCGGTCCCGATTTCTTTTTTG‑‑TCTCAGACCGCTGTCAGTCGTTCCCGTAAAGAAGCGATGATGGGCGTGCTGGGCATTACCTGCGGCGTCATGGTTTGGGCTGGGATTGCGCTGCTTGGCCTGCATTTGATTATCGAAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTgg                                                               >  1:89423/1‑191 (MQ=255)                                                                                                                                                                                                                          cTTATGAGCCTCGGTCCCGGTTTCTTTTT‑GTCTCTCAGACCGCTGTCAGTCGTTCCCGTAAAGAAGCGATGATGGGCGTGCTGGGCATTACCTGCGGCGTAATGGTTTGGGCTGGGATTGCGCTGCTTGGCCTGCATTTGATTATCGAAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGtt  >  1:9495/1‑251 (MQ=255)                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                  CAGCGAAATGCTCAGCGTTAACGGCGTTGGGATGCGCAAGCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAGTCAGCAGCATAAAAAAGTGCCAGTATGAAGACTCCGTAAACGTTTCCCCCGCGAGTCAAATGTATGTTGATGTTATTTCTCACCGTCGCCATGGTGCACATTGTGGCGCTTATGAGCCCCGGTCCCGATTTCTTTTTTGTCTCTCAGACCGCTGTCAGTCGTTCCCGTAAAGAAGCGATGATGGGCGTGCTGGGCATTACCTGCGGCGTAATGGTTTGGGCTGGGATTGCGCTGCTTGGCCTGCATTTGATTATCGAAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGTGGTGCACTGAAAAAAGAGGCGGTT  >  NC_000913/4007585‑4008053

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑