Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,896,003	T→C	I203V (ATT→GTT)	adeP ←	adenine:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,896,003	0	T	C	100.0%	35.4 / NA	13	I203V (ATT→GTT)	adeP	adenine:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (6/7);  total (6/7)

TACCGGAGGAGTCAAACAAGTTGACCAACATGAAAGAGAAAATCACCCCTGCCAGCCCGAGGTTAAACGACCCGGCTAAATCTACATGACCCACAACTGTCATTACGCTCGGCGGCGCAGAAACGATGCCATTGTAGTGCACATCACCCAGCATCCAGCCCAGCAGCGTCGTCACCACGATAGAAACCAGCACCGCTGCGTGAATGTTGCGCGAGGCCAGAATAGCAATGATGAAGAAGCCGAGGATACCCAGAAGTACGCTGTGAGAAGTCAGATTACCGATGCTCACCAGCGTTTCCGGGTTAGCGACAATCACACCTGCGTTTTTCAGCCCCATCATGCCAATGAAC  >  NC_000913/3895799‑3896148                                                                                                                                                                                                             |                                                                                                                                                  tACCGGCGGAGTCAAACAAGTTGACCAACATGAAAAAGCAAATCACCCCTGCCAGCCCGAGATTAAAAGACCCGCCTCAATCTACATGACCAACACCTGTCATTACGCTCGGCGGCGCAGACACGATGCCATTCTAGTGCACATCACCCAGCATCCAGCCCAGCAGCGTCGTCACCACGATAGAAACCAGCACCGCTGCGTGAACGTTGCGCGAGGCCAGAATAGCAATGATGAAGAAGCCGAGGATAccc                                                                                                     >  1:194058/1‑251 (MQ=255)                                               cccTGCCAGCCCGAGGTTAAACGACCCGGCTAAATCTACATGACCCACAACTGTCATTACGCTCGGCGGCGCAGAAACGATGCCATTGTAGTGCACATCACCCAGCATCCAGCCCAGCAGCGTCGTCACCACGATAGAAACCAGCACCGCTGCGTGAACGTTGCGCGAGGCCAGAATAGCAATGATGAAGAAGCCGAGGATACCCAGAAGTACGc                                                                                           >  1:85235/1‑215 (MQ=255)                                                    ccAGCCCGAGGTTAAACGACCCGGCTAAATCTACATGACCCACAACTGTCATTACGCTCGGCGGCGCAGAAACGATGCCATTGTAGTGCACATCACCCAGCATCCAGCCCAGCAGCGTCGTCACCACGATAGAAACCAGCACCGCTGCGTGAACGTTgcgc                                                                                                                                            <  1:7683/161‑1 (MQ=255)                                                                     gACCCGGCTAAATCTACATGACCCACAACTGTCTGTACGCACGGCGGCGCAGAAACGAGGCCATTGTAGTGCACAGCTCCCAGCATCCAGCCCAGCAGCGTCGTCACCACGATAGAAACCAGCCCCGCTGCGTGAACGTTGCGCGAGGCCAGAATAGCAATGATGAAGAAGCCGAGGATAc                                                                                                       <  1:1067/181‑1 (MQ=255)                                                                                                   gTCATTACGCTCGGCGGCGCAGAAACGATGCCATTGTAGTGCACATCACCCAGCATCCAGCCCAGCAGCGTCGTCACCACGATAGAAACCAGCACCGCTGCGTGAACGTTGCGCGAGGCCAGAATAGCAATGATGAAGAAGCCGAGGATACCCAGAAGTACGCTGTGAGAAGTCAGATTACCGATGCTCACCAGCGTTTCCGGGTTAGCGACAATCACACCTGc                              <  1:180096/224‑1 (MQ=255)                                                                                                     cATTACGCTCGGCGGCGCAGAAACGATGCCATTGTAGTGCACATCACCCAGCATCCAGCCCAGCAGCGTCGTCACCACGATAGAAACCAGCACCGCTGCGTGAACGTTGCGCGAGGCCAGAATAGCAATGATGAAGAAGCCGAGGATACCCAGAAGTACGCTGTGAGAAGTCAGATTACCGATGCTCACCAGCGTTTCCGGGTTAGCGACAATCACACCTGCGTTTTTCAGCCCCATCATGCCAATGAAc  >  1:87879/1‑250 (MQ=255)                                                                                                          cGCTCGGCGGCGCAGAAACGATGCCATTGTAGTGCACATCACCCAGCATCCAGCCCAGCAGCGTCGTCACCACGATAGAAACCAGCACCGCTGCGTGAACGTTGCGCGAGGCCAGAATAGCAATGATGAAGAAGCCGAGGATACCCAGAAGTACGCTGTgag                                                                                     >  1:167/1‑162 (MQ=255)                                                                                                                                 ccATTGTAGTGCACATCACCCAGCAGCCATCCCAGCAGCGGCGTCGCCACGATAGAAACCAGCACCGCTGCTTGAACGTGGCGCGAGGCCAGAATAGCAATGATGAAGAAGCCGAGGATACCCAGAAGTa                                                                                              <  1:206629/130‑1 (MQ=255)                                                                                                                                         gTGCACATCACCCAGCATCCAGCCCAGCAGCGTCGTCACCACGATAGAAACCAGCACCGCTGCGTGAACGTTGCGCGAGGCCAGAATAGCAATGATGAAGAAGCCGAGGATACCCAGAAGTACGCTGTGAGAANTCANNNNNccnn                                                                      >  2:133993/1‑144 (MQ=255)                                                                                                                                         gTGCACATCACCCAGCATCCAGCCCAGCAGCGTCGTCACCACGATAGAAACCAGCACCGCTGCGTGAACGTTGCGCGAGGCCAGAATAGCAATGATGAAGAAGCCGAGGATACCCAGAAGTACGCTGTGAGAAGTCAGATTACCGATGCTCACCAGCGTTTCCGGGtn                                                <  1:133993/168‑2 (MQ=255)                                                                                                                                                   cncnGCATCCAGCCCAGCAGCGTCGTCACCACGATAGAAACCAGCACCGCTGNGTGAACGGTGNGCGNNGCCANANTNGCAATGATGAAGAAGCCGAGGATACCCAGNAGTACGCTGTGAGAAGTCAGATTNNCGNNNCTNNCCAGc                                                           <  2:159281/147‑1 (MQ=255)                                                                                                                                                   cccAGCATCCAGCCAAGCAGCGTCGTCACCACGATAGAAACCAGCACCGCTGCGTGAACGTTGCGAGAGGCCAGAATAGCAATGATGAAGAAGCCGAGGATACCCAGAAGTACGCTGTGAGAAGTCAGATTACCGATGCTCACCAGc                                                           >  1:159281/1‑147 (MQ=255)                                                                                                                                                                                                    cNGCNTGAACNTTGCNCGAGGCCAGAATAGCNATGATGAAGAAGCCGAGGANNCNCAGAANTACGc                                                                                           <  2:85235/66‑1 (MQ=255)                                                                                                                                                                                                             |                                                                                                                                                  TACCGGAGGAGTCAAACAAGTTGACCAACATGAAAGAGAAAATCACCCCTGCCAGCCCGAGGTTAAACGACCCGGCTAAATCTACATGACCCACAACTGTCATTACGCTCGGCGGCGCAGAAACGATGCCATTGTAGTGCACATCACCCAGCATCCAGCCCAGCAGCGTCGTCACCACGATAGAAACCAGCACCGCTGCGTGAATGTTGCGCGAGGCCAGAATAGCAATGATGAAGAAGCCGAGGATACCCAGAAGTACGCTGTGAGAAGTCAGATTACCGATGCTCACCAGCGTTTCCGGGTTAGCGACAATCACACCTGCGTTTTTCAGCCCCATCATGCCAATGAAC  >  NC_000913/3895799‑3896148

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑