Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,846,964	C→T	G68G (GGG→GGA)	uhpT ←	hexose‑6‑phosphate:phosphate antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,846,964	0	C	T	100.0%	20.0 / NA	8	G68G (GGG→GGA)	uhpT	hexose‑6‑phosphate:phosphate antiporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (5/3);  total (5/3)

GTAACTGCACGAACCGCCGGTACTCTGGAAAAAGCCGCTTAAGGCGTAGAAGGCAATCATCAGGAACAGGCTAACCGAGCCGCTGCCCATACTGGCACTGAAGCCCAGCATACAAATAGCAGAGAGGATCAGCATGAACGGCAGGAATTGTTTGGTGTTTTTGCCGTCGGCGTAGTAGGAAACCAGCGTTTTACCCACGCCATAAGTGATGGAGAAACCCAGGCCGATCATCCCCAGCTGCGTCATGCTCAACCCGTAGGTCGAAATCATATCGTTCTGCGCGATGTTAAAGTTCTTGCGAATCAGGTACATCGTCAGGTAGCCGATAAAGACCACCAGGTAGGATTGCATGAACGGTTTGAACCACATTTTGCGCCGCACTTCGAGCGGAAGGTCCAGGGTCGGCTTGCGAACCTGGTT  >  NC_000913/3846733‑3847152                                                                                                                                                                                                                                        |                                                                                                                                                                                             gTAACTGCACGAACCGCCGGTACTCTGGAAAAAGCCGCTTAAGGCGTAGAAGGCAATCATCAGGAACAGGCTAACCGAGCCGCTGCCCATACTGGCACTGAAGCCCAGCATACAAATAGCAGAGAGGATCAGCATGAACGGCAGGAATTGTTTGGTGTTTTTGCCGTCGGCGTAGTAGGAAACCAGCGTTTTACCCACGCCATAAGTGATGGAGAAACCCAGGCCGATCATTCCCAGCTGCGTCATGCTc                                                                                                                                                                            >  1:182874/1‑250 (MQ=255)                                          aGGCGTAGAAGGCAATCATCAGGAACAGGCTAACCGAGCCGCTGCCCATACTGGCACTGAAGCCCAGCATACAAATAGCAGAGAGGATCAGCATGAACGGCAGGAATTGTTTGGTGTTTTTGCCGTCGGCGTAGTAGGAAACCAGCGTTTTTCCCACGCCATAAGTGATGGAGAAACCCAGGCCGATCATTCCCAGCTGCGTCATGCTCAACCCGTAGGTCGAAATCATATCGTTCTgcg                                                                                                                                             <  1:154090/240‑1 (MQ=255)                                            gCGTAGAAGGCAATCATCAGGAACAGGCTAACCGAGCCGCTGCCCATACTGCCCCTGAAGCTCAACATACAAATAGCAGAGAGGATCAGCATGAACGGCAGGAATTGTTTGGTGTTTTTGCCGTCGGCGTAGTAGGAAACCAGCGTTTTACCCACGCCATAAGTGATGGAGAAACCCAGGCCGATCATTCCCAGCTGCGTCATGCTCAACCCGTAGGTCGAAATCATATCGTTCTGCGCGATGTTAAAGtt                                                                                                                                >  1:125208/1‑251 (MQ=255)                                                                               gCCGCTGCCCATACTGGCACTGAAGCCCAGCATACAAATAGCAGAGAGGATCAGCATGAACGGCAGGCATTGTTTGGTGTTTTTGCCGTCGGCGTAGTAGGAAACCAGCGTTTTACCCACGCCATAAGTGATGGAGAAACCCAGGCCGATCATTCCCAGCTGCGTCATGCTCAACCCGTCGGTCGAAATCATATCGTTCTGCGCGATGTTAAAGTTCTTGCGAATCAGATACATCGTCAGGTAGCCGATa                                                                                              >  1:156039/1‑250 (MQ=255)                                                                                                                                                                      gTCGGCGTAGTAGGAAACCAGCGTTTTACCCACGCCATAAGTGATGGAGAAACCCAGGCCGATCATTCCCAGCTGCGTCATGCTCAACCCGTAGGTCGAAATCATATCGTTCTGCGCGATGTTAAAGTTCTTGCGAATCAGGTACATCGTCAGGTAGCCGATAAAGACCACCAgg                                                                                  <  1:60177/175‑1 (MQ=255)                                                                                                                                                                          gCGTAGTAGGAAACCAGCGTTTTACCCACGCCATAAGTGATGGAGAAACCCAGGCCGATCATTCCCAGCTGCGTCATGCTCAACCCGTAGGTCGAAATCATATCGTTCTGCGCGATGTTAAAGTTCTTGCGAATCAGGTACATCGTCAGGTAGCCGATAAAGACCACCAGGTAGGATTGCATGAACGGTTTGAACCACATTTTGCGCCGCACTTCGAGCGGAAGGTCCAGGGTCGGCTTGCGAACCTGGtt  >  1:117002/1‑251 (MQ=255)                                                                                                                                                                                  ggAAACCAGCGTTTTACCCACGCCATAAGTGATGGAGAAACCCAGGCCGCTCCTTCCCAGCTGCGTCATGCTCAACCCGTCGGTCGAAATCATATCGTTCTGCGCGATGTTAAAGTTCTTGCGAATCAGGTACATCGTCAGGTAGCCGa                                                                                                >  1:121962/1‑149 (MQ=255)                                                                                                                                                                                                                gATGGAGAAACCCAGGCCGATCATTCCCAGCTGCGTCATGCTCAACCCGTAGGTCGAAATCATATCGTTCTGCGCGATGTTAAAGTTCTTGCGAATCAGGTACATCGTCAGGTAGCCGATAAAGACCACCAGGTAGGATTGCATGAACGGTTTGAACCACATTTTGCGCCGCACTTCGAGCg                                 <  1:119746/182‑1 (MQ=255)                                                                                                                                                                                                                                        |                                                                                                                                                                                             GTAACTGCACGAACCGCCGGTACTCTGGAAAAAGCCGCTTAAGGCGTAGAAGGCAATCATCAGGAACAGGCTAACCGAGCCGCTGCCCATACTGGCACTGAAGCCCAGCATACAAATAGCAGAGAGGATCAGCATGAACGGCAGGAATTGTTTGGTGTTTTTGCCGTCGGCGTAGTAGGAAACCAGCGTTTTACCCACGCCATAAGTGATGGAGAAACCCAGGCCGATCATCCCCAGCTGCGTCATGCTCAACCCGTAGGTCGAAATCATATCGTTCTGCGCGATGTTAAAGTTCTTGCGAATCAGGTACATCGTCAGGTAGCCGATAAAGACCACCAGGTAGGATTGCATGAACGGTTTGAACCACATTTTGCGCCGCACTTCGAGCGGAAGGTCCAGGGTCGGCTTGCGAACCTGGTT  >  NC_000913/3846733‑3847152

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑