Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,758,882	G→A	S327F (TCT→TTT)	selB ←	selenocysteyl‑tRNA‑specific translation elongation factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,758,882	0	G	A	100.0%	15.7 / NA	9	S327F (TCT→TTT)	selB	selenocysteyl‑tRNA‑specific translation elongation factor
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (7/2);  total (7/2)

GCGCATCCCTTCGCCGTTGAGCTGGCGCGCCCAGGCGAAATCCGCAAGGTTAACCGCGCCGCGTTCCAGATGAACAGATAACGCATCGGCATCGCTCTGCGCCCGTGCAAGAGACGCCAGCCATTGCAGATATTCCGGCTTACGTTTACCGCGACGCGGCGGGTTAAGCATCACGACGCGCGCTCCGGCCAGCGTGTTGCGGGCAGAGATATCGCGCAATACCAGGCGGTCGTTATCTGCCAGCCATAACGGGGTGTCGAAGACCAGTTCAGCAAGGTTATCTTCCAGCAGTGAAACGCGTCCCGTGACGTGGCTGGCGGCGTGGTGAATATGCAGCGGCTGCCACTGGGTCAGCGGTGTATGGGTTTGAAGCTCGACAATCACCCGTGTGAACGGCTCTGGCGGCACATCGGCAAGCAGCCAGTCGCCACGGTTAATCTGCTCT  >  NC_000913/3758677‑3759121                                                                                                                                                                                                              |                                                                                                                                                                                                                                                gcgcATCCCTTCGCCGTTGCGCTGGCGAGCACAGGCGAAATCCGCAAGGTTAACCGCGCCGCGTTCCAGATGAACAGATAACGCATCGGCATCGCTCTGCGCCCGTGCAAGAGACGCCAGCCATTGCAGATATTCCGGCTTACGTTTACCGCGACGCGGCGGGTTAAGCATCACGACGCGCGCTCCGGCCAGCGTGGTGCGGGGAAAGATAACGCGCAATACCAGGCGGTCGTTATCTGCCAGCCATAACg                                                                                                                                                                                                    >  1:61809/1‑251 (MQ=255)                          cgcgcCCAGGCGAAATCCGCAAGGTTAACCGCGCCGCGTTCCAGATGAACAGATAACGCATCGGCATCGCTCTGCGCCCGTGCAAGAGACGCCAGCCATTGCAGATATTCCGGCTTACGTTTACCGCGACGCGGCGGGTTAAGCATCATGACGCGCGCTCCGGCCCGCGTGTTGCGGGCAAAGATATCGCGCAATACCAGGCGGTCGGTATCTGCCCGCCATAACGGGGGGTCGAAGACCAGTTCAGCa                                                                                                                                                                             >  1:219543/1‑249 (MQ=255)                                cAGGCGAAATCCGCAAGGTTAACCGCGCCGCGTTCCAGATGAACAGATAACGCATCGGCATCGCTCTGCGCCCGTGCAAGAGACGCCAGCCATTGCAGATATTCCGGCTTACGTTTACCGCGACGCGGCGGGTTAAGCATCACGACGCGCGCCCCGGCCAGCGTGTTGCGGGCAAAGATATCGCGCAATACCAGGCGGGCGTTATCTgccagcccta                                                                                                                                                                                                       >  1:110305/1‑214 (MQ=255)                                          cctcaatgTTAACCGCGCCGCGGTCCAGTTGAACAGATAACGCAACGGCATCGCTCTGCGCCCGTGCAAGAGACGCCAGCCATTGCAGATATTCCGGCTTACGTTTACCGCGACGCGGCGGTTTAAGCATCACGACGCGCGCTCCGGCCAGCGTGTTTCGGGCAAAGAGACCGCGCAATACCAGGCGGTCGTGATCTGCCAGCCATATCGGGGGGTCGAAGACCAGTTCAGCAAGGTTATCTTCCAGCAGt                                                                                                                                                           <  1:106015/244‑1 (MQ=255)                                                                                                      cccGTGCAAGAGACGCCAGCCATTGCAGATATTCCGGCTTACGTTTACCGCGACGCGGCGGGTTAACCATCACGACGCGCGCTCCGGCCAGCGTGTTGCGGGCAAAGATATCGCGCAATACCAGGCGGTCGTTATCTGCCAGCCATAACGGGGTGTCGCAGACCAGTTCAGCAAGGTTATCTTCCAGCAGTGAAACGCGTCCCGTGACGTGGCTGGCGGCGTGGGGAATATGAAGCGGCTGCCACTGGGTc                                                                                               >  1:154654/1‑251 (MQ=255)                                                                                                                                                         gACGCGGCGGGTTAAGCATCACGACGCGCGCTCCGGCCAGCGTGTTGCGGGCAAAGATATCGCGCAATACCAGGCGGTCGTTATCTGCCAGCCATAACGGGGTGTCGAAGACCAGTTCAGCAAGGTTATCTTCCAGCAGTGAAACGCGTCCCGTGACGTGGCTGGCGGCGTGGTGAATATGCAGCGGCTGCCACTGGGTCAGCGGTGTATGGGTTGGAAGCTCGACAATCACCCGTGTGAACGGCTCTggc                                            <  1:73820/251‑1 (MQ=255)                                                                                                                                                              ggcggGATAAGCATCACGACGCGCGCTCCGGCCAGCGTGTTGCGGGCCAAGATATCGCGCAATACCAGGCGGTCGTTATCTGCCAGCCATAACGGGGTGTCGAAGACCCGTTCACCAAGGTTATCTTCcagccncgcnnnnngtnn                                                                                                                                                >  2:128683/1‑132 (MQ=255)                                                                                                                                                                       aGCATCACGACGCGCGCTCCGGCCAGCGTGTTGCGGGCAAAGATATCGCGCTATACCAGGCGGTCGTTATCTGCCAGCCATAACGGGGTGACGAAGACCAGTTCAGCAAGGTTATCTTCCAGCAGTGAAACGCGTCCCGTGACGTGGCTGGCGGCGTGGTGAATATGCAGCGGCTGCCACTGGGTCAGCGGTGTCTGGGTTTGAAGCTCGACAATCACCCGTGTGAACGGCTCTGGCGGCACATCGGCa                                >  1:190285/1‑249 (MQ=255)                                                                                                                                                                                                   tgtTGCGGGCAAAGATATCGCGCAATACCAGGCGGTCGTTATCTGCCAGCCATAAACGGGTGTCGAAGACCAGTTCAGCAAGGTTCTCTTCCAGCAGTGAAACGCGTCCCGTGACGTGGCTGGCGGCGTGGTGAATATGCAGCGGCTGCCACTGGGTCAGCGGTGTATGGGTTTGAAGCTCGACAATCACCCGTGTGAACGGCTCTGGCGGCACATCGGCAAGCAGCCAGTCGCCACGGTTAATCTGctct  >  1:118871/1‑251 (MQ=255)                                                                                                                                                                                                              |                                                                                                                                                                                                                                                GCGCATCCCTTCGCCGTTGAGCTGGCGCGCCCAGGCGAAATCCGCAAGGTTAACCGCGCCGCGTTCCAGATGAACAGATAACGCATCGGCATCGCTCTGCGCCCGTGCAAGAGACGCCAGCCATTGCAGATATTCCGGCTTACGTTTACCGCGACGCGGCGGGTTAAGCATCACGACGCGCGCTCCGGCCAGCGTGTTGCGGGCAGAGATATCGCGCAATACCAGGCGGTCGTTATCTGCCAGCCATAACGGGGTGTCGAAGACCAGTTCAGCAAGGTTATCTTCCAGCAGTGAAACGCGTCCCGTGACGTGGCTGGCGGCGTGGTGAATATGCAGCGGCTGCCACTGGGTCAGCGGTGTATGGGTTTGAAGCTCGACAATCACCCGTGTGAACGGCTCTGGCGGCACATCGGCAAGCAGCCAGTCGCCACGGTTAATCTGCTCT  >  NC_000913/3758677‑3759121

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑