Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,486,205	C→A	T29K (ACG→AAG)	crp →	DNA‑binding transcriptional dual regulator CRP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,486,205	0	C	A	100.0%	27.6 / NA	11	T29K (ACG→AAG)	crp	DNA‑binding transcriptional dual regulator CRP
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (7/4);  total (7/4)

GATGTACTGCATGTATGCAAAGGACGTCACATTACCGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGA‑TCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAAC  >  NC_000913/3485972‑3486449                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                      gaggTACTTCATGTATGCAAAGGACGTCCCATTACCGTGCAGTACAGGTGATAGCCCCTTCCCAGGTAGCGGGAATCATATTTCGGCAATCCAGAGACAGCGGCGTCATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCGCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTg                                                                                                                                                                                                                                      <  1:148213/248‑1 (MQ=255)                              caTTACAGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGAACTCCAGAGACAGCGGAGTTATCTGGCTCTGGCGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGtt                                                                                                                                                                                                         >  1:198856/1‑251 (MQ=255)                                                  gATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCTGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCt                                                                                                                                                                                     >  1:117033/1‑251 (MQ=255)                                                                         gAAGCCTATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGA‑TCaaa                                                                                                                                                                             >  1:173323/1‑235 (MQ=255)                                                                                                                           aaGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGA‑TCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGc                                                                                                           <  1:89005/251‑1 (MQ=255)                                                                                                                                                           cTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATCAGTACCCATCCAAGCGCAAGCTTATTAACCAGGGAGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGC‑TCAAAGACGAAGAGGGTAAAGAAATAATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGCACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATg                                                                           >  1:142467/1‑251 (MQ=255)                                                                                                                                                                                                 cTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGA‑TCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAAttt                                     >  1:184483/1‑251 (MQ=255)                                                                                                                                                                                                        naTTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTg                                                                                                                                                                                                                                      >  1:132504/2‑52 (MQ=255)                                                                                                                                                                                                        cATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTg                                                                                                                                                                                                                                      <  2:132504/52‑1 (MQ=255)                                                                                                                                                                                                                               aTCCAAGAGCAAGCTTATCCCCCAGGTTGAAAAAGCGGAAACGCTGTACGACATCGTTAAAGGCTTTGTGGCAGTG‑TGAATCAAATACGAAGAGGGTAAAGAACTGACCCTCTCTTATCGGAAGCAGTGTGATTTTATTGGCGAACTGGGCTTGTTGGAAGGGGGCCAGGAACTTAGCGCAGGGGTCCGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTGCGCCAATTGATTCAgg       <  1:69794/251‑1 (MQ=255)                                                                                                                                                                                                                                    agagCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAACGGCTCTGTGGCAGTGCTGA‑TCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAAc  >  1:219479/1‑251 (MQ=255)                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                      GATGTACTGCATGTATGCAAAGGACGTCACATTACCGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGA‑TCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAAC  >  NC_000913/3485972‑3486449

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑